{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T21:10:23Z","timestamp":1721423423234},"reference-count":40,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1994,4,1]],"date-time":"1994-04-01T00:00:00Z","timestamp":765158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The effect of interleukin-1\u03b1 (IL-1\u03b1) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hours with IL-1\u03b1 in combination with EGF. Most enzyme appeared to be associated with the extracellular matrix, but labeling was also found in numerous fibroblast-like cells. Explants cultured in the presence of IL-1\u03b1 alone contained less enzyme and in periostea treated without cytokines, or with EGF alone, only a faint label, if any, was seen. Freshly isolated, non-cultured periostea contained no detectable enzyme.<\/jats:p>\n               <jats:p>Extraction of collagenase from periostea revealed that: (1) non-cultured periosteum did not contain detectable levels of enzyme. (2) The amount of total activatable enzyme synergistically increased (10-fold) under the influence of IL-1\u03b1 and EGF, whereas IL-1\u03b1 alone showed a 4-fold enhancement compared to control or EGF-incubated explants. (3) The latent fraction of the enzyme was synergistically increased (up to 100-fold or more) in periostea cultured in the presence of IL-1\u03b1+EGF (21.17 mU\/explant versus 0.05 mU\/explant in controls). (4) Active collagenase, on the other hand, appeared to be present in a relatively high concentration in explants cultured without cytokines (2.45 mU\/explant versus 0.36 mU\/explant in IL-1\u03b1+EGF-treated explants). (5) No enzyme could be extracted from devitalized explants that were incubated in media containing high levels of latent collagenase. It was concluded that collagenase had been incorporated somehow in the extracellular matrix during culturing. In an attempt to analyze breakdown of collagen the amount of hydroxyproline in culture media was assessed. The level of this imino acid released by periostea treated without cytokines or with EGF alone was significantly higher than by those incubated with IL-1\u03b1 and EGF.<\/jats:p>\n               <jats:p>In conclusion, incorporation of collagenase in periosteal connective tissue seems to occur during culturing; the level of the incorporated latent enzyme being strongly enhanced by a combination of the cytokines IL-1\u03b1 and EGF. However, as the amount of collagen breakdown had decreased, these findings indicate that IL-1\u03b1 in combination with EGF is able to induce deposition of a large reservoir of latent collagenase in the extracellular matrix, but does not stimulate adequate activation mechanisms to result in enhanced degradation.<\/jats:p>","DOI":"10.1242\/jcs.107.4.1047","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T02:27:28Z","timestamp":1619231248000},"page":"1047-1053","source":"Crossref","is-referenced-by-count":12,"title":["immunolocalisation of collagenase in rabbit periosteal tissue explants and extraction of the enzyme: the effect of the cytokines IL-1\u03b1 and EGF"],"prefix":"10.1242","volume":"107","author":[{"given":"Erwin","family":"van der Zee","sequence":"first","affiliation":[{"name":"1 Experimental Oral Biology Group, Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Everts","sequence":"additional","affiliation":[{"name":"1 Experimental Oral Biology Group, Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA)"},{"name":"University of Amsterdam 2 Laboratory of Cell Biology and Histology, Academic Medical Centre , , Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kees","family":"Hoeben","sequence":"additional","affiliation":[{"name":"University of Amsterdam 2 Laboratory of Cell Biology and Histology, Academic Medical Centre , , Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter","family":"Beertsen","sequence":"additional","affiliation":[{"name":"1 Experimental Oral Biology Group, Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1994,4,1]]},"reference":[{"key":"2024022706194877000_JOCES_107_4_1047C1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0014-4827(92)90386-M","article-title":"Culture of human dermal fibroblasts in collagen gels: modulation of interleukin 1-induced prostaglandin E2 synthesis by an extracellular matrix","volume":"198","author":"Barr","year":"1992","journal-title":"Exp. 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