{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:07:11Z","timestamp":1791295631049,"version":"4.1.0"},"reference-count":47,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1995,1,1]],"date-time":"1995-01-01T00:00:00Z","timestamp":788918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells expressing cot gene were identified: (1) granular duct cells in the submandibular and sublingual glands; (2) serous cells in the parotid gland; (3) peptic (chief) cells in gastric glands; and (4) goblet cells in colonic glands. Investigation of the developmentally regulated expression of cot mRNA using tissues of 14-day and 18-day embryos, newborn and weanling mice showed that cot gene is expressed only in morphologically differentiated and functionally activated cells of these four types. No other types of cells showing ISH signals were observed. Based on these results, cot gene expressions in cultured cells of colonic adenocarcinomas and gastric adenocarcinomas were examined. SW 480 and WiDr cells showed high expression of this gene and so should be useful for functional analysis of Cot kinase. The expression patterns of cot gene in tumor tissues of the parotid gland, and gastric and colonic glands were investigated. Two of the tissues over-expressed this gene markedly, suggesting that overproduction of Cot kinase may be one cause of their transformation.<\/jats:p>","DOI":"10.1242\/jcs.108.1.97","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:10:34Z","timestamp":1619367034000},"page":"97-103","source":"Crossref","is-referenced-by-count":24,"title":["Identification of the cells expressing <i>cot<\/i> proto-oncogene mRNA"],"prefix":"10.1242","volume":"108","author":[{"given":"Reiko","family":"Ohara","sequence":"first","affiliation":[{"name":"1 Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichi","family":"Hirota","sequence":"additional","affiliation":[{"name":"Osaka University Medical School, Yamadaoka 2-2 2 Department of Pathology , , Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Onoue","sequence":"additional","affiliation":[{"name":"Osaka University Medical School, Yamadaoka 2-2 2 Department of Pathology , , Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Nomura","sequence":"additional","affiliation":[{"name":"Osaka University Medical School, Yamadaoka 2-2 2 Department of Pathology , , Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiko","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Osaka University Medical School, Yamadaoka 2-2 2 Department of Pathology , , Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumao","family":"Toyoshima","sequence":"additional","affiliation":[{"name":"1 Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita 565, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1995,1,1]]},"reference":[{"key":"2023122400533057000_JOCES_108_1_97C1","first-page":"1515","article-title":"Identification and characterization of protein products of the cot oncogene with serine kinase activity","volume":"6","author":"Aoki","year":"1991","journal-title":"Oncogene"},{"key":"2023122400533057000_JOCES_108_1_97C2","doi-asserted-by":"publisher","first-page":"22723","DOI":"10.1016\/S0021-9258(18)41587-6","article-title":"The human cot proto-oncogene encodes two protein serine\/threonine kinases with different transforming activities by alternative initiation of translation","volume":"30","author":"Aoki","year":"1993","journal-title":"J. Biol. Chem"},{"key":"2023122400533057000_JOCES_108_1_97C3","first-page":"6296","article-title":"Primary structure, expression, and signal-dependent tyrosine phosphorylation of a Drosophila homolog of extracellular signal-regulated kinase","volume":"89","author":"Biggs","year":"1991","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400533057000_JOCES_108_1_97C4","first-page":"274","article-title":"A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region","volume":"254","author":"Bellacosa","year":"1991","journal-title":"Science"},{"key":"2023122400533057000_JOCES_108_1_97C5","doi-asserted-by":"publisher","first-page":"5889","DOI":"10.1073\/pnas.90.13.5889","article-title":"Signal transduction via the MAP kinases: proceed at your own RSK","volume":"90","author":"Blenis","year":"1993","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400533057000_JOCES_108_1_97C6","first-page":"1400","article-title":"Structure and biological activity of human homolog of the raf\/mil oncogene","volume":"5","author":"Bonner","year":"1985","journal-title":"Mol. Cell. Biol"},{"key":"2023122400533057000_JOCES_108_1_97C7","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1093\/nar\/14.2.1009","article-title":"The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene","volume":"12","author":"Bonner","year":"1986","journal-title":"Nucl. Acids Res"},{"key":"2023122400533057000_JOCES_108_1_97C8","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/0092-8674(91)90639-G","article-title":"Oncogenes and signal transduction","volume":"64","author":"Cantley","year":"1991","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C9","first-page":"1329","article-title":"Expression cDNA cloning of a serine kinase transforming gene","volume":"8","author":"Chan","year":"1993","journal-title":"Oncogene"},{"key":"2023122400533057000_JOCES_108_1_97C10","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem"},{"key":"2023122400533057000_JOCES_108_1_97C11","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1016\/0092-8674(89)90509-6","article-title":"A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae","volume":"58","author":"Courchense","year":"1989","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C12","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.1126\/science.1326789","article-title":"Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro","volume":"257","author":"Dent","year":"1992","journal-title":"Science"},{"key":"2023122400533057000_JOCES_108_1_97C13","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1038\/307521a0","article-title":"Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences","volume":"307","author":"Downward","year":"1984","journal-title":"Nature"},{"key":"2023122400533057000_JOCES_108_1_97C14","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1016\/0092-8674(90)90668-5","article-title":"FUS3 encodes a cdc2+\/CDC28-related kinase required for the transition from mitosis into conjugation","volume":"60","author":"Elion","year":"1990","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C15","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/0076-6879(91)00126-H","article-title":"Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members","volume":"200","author":"Hanks","year":"1991","journal-title":"Meth. Enzymol"},{"key":"2023122400533057000_JOCES_108_1_97C16","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0169-328X(92)90150-A","article-title":"Localization of mRNA for c-kit receptor and its ligand in the brain of adult rats: an analysis using in situ hybridization histochemistry","volume":"15","author":"Hirota","year":"1992","journal-title":"Mol. Brain Res"},{"key":"2023122400533057000_JOCES_108_1_97C17","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/0092-8674(92)90361-F","article-title":"Activation of the MAP kinase pathway by the protein kinase raf","volume":"71","author":"Howe","year":"1992","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C18","doi-asserted-by":"publisher","first-page":"4171","DOI":"10.1073\/pnas.88.10.4171","article-title":"Molecular cloning and identification of a serine\/threonine protein kinase of the second-messenger subfamily","volume":"88","author":"Jones","year":"1991","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400533057000_JOCES_108_1_97C19","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1146\/annurev.cb.03.110187.000335","article-title":"Cell transformation by the viral src oncogene","volume":"3","author":"Jove","year":"1987","journal-title":"Annu. Rev. Cell Biol"},{"key":"2023122400533057000_JOCES_108_1_97C20","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/358417a0","article-title":"Raf-1 activates MAP kinase-kinase","volume":"358","author":"Kariakis","year":"1992","journal-title":"Nature"},{"key":"2023122400533057000_JOCES_108_1_97C21","volume-title":"The Atlas of Mouse Development","author":"Kaufman","year":"1992"},{"key":"2023122400533057000_JOCES_108_1_97C22","doi-asserted-by":"publisher","first-page":"2903","DOI":"10.1002\/j.1460-2075.1992.tb05359.x","article-title":"Xenopus MAP kinase activator is a serine\/threonine kinase activated by threonine phosphorylation","volume":"11","author":"Kosako","year":"1992","journal-title":"EMBO J"},{"key":"2023122400533057000_JOCES_108_1_97C23","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1002\/j.1460-2075.1993.tb05713.x","article-title":"cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates","volume":"12","author":"Kosako","year":"1993","journal-title":"EMBO J"},{"key":"2023122400533057000_JOCES_108_1_97C24","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1126\/science.8385802","article-title":"A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf","volume":"260","author":"Lange-Carter","year":"1993","journal-title":"Science"},{"key":"2023122400533057000_JOCES_108_1_97C25","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1016\/0092-8674(91)90640-K","article-title":"Oncogenic conversion by regulatory changes in transcription factors","volume":"64","author":"Lewin","year":"1991","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C26","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1002\/j.1460-2075.1992.tb05136.x","article-title":"Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation","volume":"11","author":"Matsuda","year":"1992","journal-title":"EMBO J"},{"key":"2023122400533057000_JOCES_108_1_97C27","first-page":"4088","article-title":"Structure and transforming potential of the human cot oncogene encoding a putative protein kinase","volume":"11","author":"Miyoshi","year":"1991","journal-title":"Mol. Cell. Biol"},{"key":"2023122400533057000_JOCES_108_1_97C28","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1002\/j.1460-2075.1988.tb02905.x","article-title":"A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeast","volume":"7","author":"Nadin-Davis","year":"1988","journal-title":"EMBO J"},{"key":"2023122400533057000_JOCES_108_1_97C29","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1083\/jcb.106.2.441","article-title":"Developmental expression of 2ar (osteopontin) and SPARAC (osteonectin) RNA as revealed by in situ hybridization","volume":"106","author":"Nomura","year":"1988","journal-title":"J. Cell Biol"},{"key":"2023122400533057000_JOCES_108_1_97C30","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1111\/j.1349-7006.1993.tb00170.x","article-title":"The murine cot proto-oncogene : genome structure and tissue-specific expression","volume":"84","author":"Ohara","year":"1993","journal-title":"Jpn J. Cancer Res"},{"key":"2023122400533057000_JOCES_108_1_97C31","first-page":"2486","article-title":"The human PIM-1 gene product is a protein serine kinase","volume":"51","author":"Padma","year":"1991","journal-title":"Cancer Res"},{"key":"2023122400533057000_JOCES_108_1_97C32","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1126\/science.1382311","article-title":"MAP kinases : charting the regulatory pathways","volume":"257","author":"Pelech","year":"1992","journal-title":"Science"},{"key":"2023122400533057000_JOCES_108_1_97C33","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1038\/300149a0","article-title":"A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene","volume":"300","author":"Reddy","year":"1982","journal-title":"Nature"},{"key":"2023122400533057000_JOCES_108_1_97C34","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1101\/gad.4.11.1862","article-title":"STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast","volume":"4","author":"Rhodes","year":"1990","journal-title":"Genes Dev"},{"key":"2023122400533057000_JOCES_108_1_97C35","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/335519a0","article-title":"Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes","volume":"335","author":"Sagata","year":"1988","journal-title":"Nature"},{"key":"2023122400533057000_JOCES_108_1_97C36","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1038\/342512a0","article-title":"The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs","volume":"342","author":"Sagata","year":"1989","journal-title":"Nature"},{"key":"2023122400533057000_JOCES_108_1_97C37","first-page":"923","article-title":"Chicken homolog of the mos proto-oncogene","volume":"8","author":"Schmidt","year":"1988","journal-title":"Mol. Cell. Biol"},{"key":"2023122400533057000_JOCES_108_1_97C38","doi-asserted-by":"publisher","first-page":"14373","DOI":"10.1016\/S0021-9258(19)49722-6","article-title":"Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A 431 cells","volume":"267","author":"Seger","year":"1992","journal-title":"J. Biol. Chem"},{"key":"2023122400533057000_JOCES_108_1_97C39","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/0092-8674(86)90886-X","article-title":"The primary structure of the putative oncogene pim-1 shows extensive homology with protein kinases","volume":"46","author":"Selton","year":"1986","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C40","first-page":"1","article-title":"Development of the salivary glands","volume-title":"The Salivary System","author":"Sreebny","year":"1987"},{"key":"2023122400533057000_JOCES_108_1_97C41","doi-asserted-by":"publisher","first-page":"7371","DOI":"10.1073\/pnas.83.19.7371","article-title":"Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases","volume":"83","author":"Teague","year":"1986","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400533057000_JOCES_108_1_97C42","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1101\/gad.5.1.60","article-title":"Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK\/MAP 2 and budding yeast FUS3 and KSS1 kinases","volume":"5","author":"Toda","year":"1991","journal-title":"Genes Dev"},{"key":"2023122400533057000_JOCES_108_1_97C43","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/0092-8674(93)90117-9","article-title":"A protein kinase similar to MAP kinase activator acts downstream of the Raf kinase in Drosophila","volume":"72","author":"Tsuda","year":"1993","journal-title":"Cell"},{"key":"2023122400533057000_JOCES_108_1_97C44","first-page":"3554","article-title":"byr2, a Schizosaccahromyces pombe gene encoding a protein kinase capable of partial suppression of the ras 1 mutant phenotype","volume":"11","author":"Wang","year":"1991","journal-title":"Mol. Cell. Biol"},{"key":"2023122400533057000_JOCES_108_1_97C45","doi-asserted-by":"publisher","first-page":"4078","DOI":"10.1073\/pnas.79.13.4078","article-title":"Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus","volume":"79","author":"Watson","year":"1982","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400533057000_JOCES_108_1_97C46","volume-title":"Functional Histology","author":"Wheater","year":"1987"},{"key":"2023122400533057000_JOCES_108_1_97C47","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1073\/pnas.90.1.173","article-title":"Molecular structure of a protein-tyrosine\/threonine kinase activation p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase","volume":"90","author":"Wu","year":"1993","journal-title":"Proc. Nat. Acad. Sci. USA"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/108\/1\/97\/3323860\/joces_108_1_97.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/108\/1\/97\/3323860\/joces_108_1_97.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T00:53:46Z","timestamp":1703379226000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/108\/1\/97\/24368\/Identification-of-the-cells-expressing-cot-proto"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.108.1.97","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1,1]]}}}