{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T05:03:33Z","timestamp":1703394213153},"reference-count":52,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1995,2,1]],"date-time":"1995-02-01T00:00:00Z","timestamp":791596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The phenotype of the bnbn hemolytic anemia mutation in the domestic turkey is manifested as binucleation specifically in the definitive erythrocyte lineage, most likely as the consequence of anomolous centrosomal activity (Bloom et al., 1970; Searle and Bloom, 1979). Here we have identified in turkey two variants of the novel, centrosomally-associated erythroid-specific protein p23. One variant is Ca2+-sensitive and is highly homologous to its chick counterpart (Zhu et al., 1995, accompanying paper). The other, p21, is a truncated form resulting from a 62 amino acid deletion from the 3\u2032 end and a 40 amino acid insertion at the 5\u2032 end, and appears to lack Ca2+-sensitivity. These proteins are localized at the marginal band, centrosomes and nuclear membrane of differentiated erythrocytes. Anti-p23\/p21 immunofluorescence revealed the presence of multiple centrosomes in bnbn eythrocytes. We therefore undertook a detailed genetic analysis to determine whether the p21 variant represented the bn mutation. Initial tests of normal BnBn and mutant bnbn individuals suggested that the p23\/p21 proteins might be encoded by the Bn\/bn genes. However, further genetic tests demonstrated independent segregation for these two genetic loci. Thus, these proteins are encoded by the heretofore undescribed genes, p23\/p21, mapping to an autosomal locus in the turkey genome.<\/jats:p>","DOI":"10.1242\/jcs.108.2.699","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T02:45:48Z","timestamp":1619232348000},"page":"699-710","source":"Crossref","is-referenced-by-count":2,"title":["Novel centrosomal protein reveals the presence of multiple centrosomes in turkey (<i>Meleagris Gallopavo<\/i>) <i>bnbn<\/i> binucleated erythrocytes"],"prefix":"10.1242","volume":"108","author":[{"given":"Catherine M.","family":"Woods","sequence":"first","affiliation":[{"name":"1 Department of Pharmacology, Merck Research Laboratories, West Point, PA 19486, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhu","sequence":"additional","affiliation":[{"name":"1 Department of Pharmacology, Merck Research Laboratories, West Point, PA 19486, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Coleman","sequence":"additional","affiliation":[{"name":"2 Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen E.","family":"Bloom","sequence":"additional","affiliation":[{"name":"3 Department of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, NY 14853-5601, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elias","family":"Lazarides","sequence":"additional","affiliation":[{"name":"1 Department of Pharmacology, Merck Research Laboratories, West Point, PA 19486, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,2,1]]},"reference":[{"key":"2023122400553433100_JOCES_108_2_699C1","doi-asserted-by":"publisher","first-page":"6970","DOI":"10.1073\/pnas.84.20.6970","article-title":"Internal amino acid sequence analysis of proteins seperated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose","volume":"83","author":"Aebersold","year":"1987","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400553433100_JOCES_108_2_699C2","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1038\/355300a0","article-title":"Centrosome and cell division","volume":"355","author":"Bailly","year":"1992","journal-title":"Nature"},{"key":"2023122400553433100_JOCES_108_2_699C3","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1083\/jcb.124.5.783","article-title":"PCM-1, a 228-kD centrosome autoantigen with a distinct cell cycle distribution","volume":"124","author":"Balczon","year":"1994","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C4","doi-asserted-by":"publisher","first-page":"2669","DOI":"10.1083\/jcb.107.6.2669","article-title":"Identification and localization of a novel, cytoskeletal, centrosome-associated protein in PtK2 cells","volume":"107","author":"Baron","year":"1988","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C5","doi-asserted-by":"publisher","first-page":"5512","DOI":"10.1073\/pnas.83.15.5512","article-title":"Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins","volume":"83","author":"Baum","year":"1986","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400553433100_JOCES_108_2_699C6","first-page":"5386","article-title":"A yeast gene essential for regulation of spindle pole duplication","volume":"8","author":"Baum","year":"1988","journal-title":"Mol. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C7","doi-asserted-by":"publisher","first-page":"1803","DOI":"10.1083\/jcb.96.6.1803","article-title":"Vimentin filaments are assembled from a soluble precursor in avian erythroid cells","volume":"96","author":"Blikstadt","year":"1983","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C8","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1093\/genetics\/65.1.51","article-title":"Cytological and cytophotometric analysis of binucleated red blood cell mutants (bn) in turkeys (Meleagris gallopavo)","volume":"65","author":"Bloom","year":"1970","journal-title":"Genetics"},{"key":"2023122400553433100_JOCES_108_2_699C9","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1146\/annurev.cb.01.110185.001045","article-title":"Microtubule organizing centers","volume":"1","author":"Brinkley","year":"1985","journal-title":"Annu. Rev. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C10","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/0005-2795(80)90254-8","article-title":"Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate","volume":"623","author":"Burgess","year":"1980","journal-title":"Biochim. Biophys. Acta"},{"key":"2023122400553433100_JOCES_108_2_699C11","doi-asserted-by":"publisher","first-page":"4882","DOI":"10.1073\/pnas.90.11.4882","article-title":"Identification and cloning of a protein kinase-encoding mouse gene, Plk, related to the polo gene of Drosophila","volume":"90","author":"Clay","year":"1993","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400553433100_JOCES_108_2_699C12","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/0092-8674(94)90504-5","article-title":"Pericentrin, a highly conserved centrosome protein involved in microtubule organization","volume":"76","author":"Doxsey","year":"1994","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C13","first-page":"249","article-title":"Polarity of marginal band microtubules in vertebrate erythrocytes","volume":"37","author":"Eutenauer","year":"1985","journal-title":"Eur. J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C14","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1083\/jcb.124.1.19","article-title":"Centrosome assembly in vitro: role of \u03b3-tubulin recruitment in Xenopus sperm aster formation","volume":"124","author":"F\u00e9lix","year":"1994","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C15","doi-asserted-by":"publisher","first-page":"2033","DOI":"10.1083\/jcb.110.6.2033","article-title":"Centrosome duplication continues in cycloheximide-treated Xenopus blastulas in the absence of a detectable cell-cycle","volume":"110","author":"Gard","year":"1990","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C16","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1242\/jcs.96.4.605","article-title":"Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts","volume":"96","author":"Gonzalez","year":"1990","journal-title":"J. Cell Sci"},{"key":"2023122400553433100_JOCES_108_2_699C17","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1083\/jcb.73.3.601","article-title":"The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation","volume":"73","author":"Gould","year":"1977","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C18","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/0092-8674(82)90032-0","article-title":"Structural association of synemin and vimentin filaments in avian erythrocytes revealed by immunoelectron microscopy","volume":"30","author":"Granger","year":"1982","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C19","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1242\/jcs.99.4.693","article-title":"The fission yeast-tubulin is essential for mitosis and is localized at microtubule organizing centers","volume":"99","author":"Horio","year":"1991","journal-title":"J. Cell Sci"},{"key":"2023122400553433100_JOCES_108_2_699C20","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1083\/jcb.107.1.133","article-title":"Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+-binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product","volume":"107","author":"Huang","year":"1988","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C21","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1038\/356080a0","article-title":"\u03b3-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation","volume":"356","author":"Joshi","year":"1992","journal-title":"Nature"},{"key":"2023122400553433100_JOCES_108_2_699C22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0074-7696(08)62049-5","article-title":"Functional components of microtubule-organizing centers","volume":"136","author":"Kimble","year":"1992","journal-title":"Int. Rev. Cyto"},{"key":"2023122400553433100_JOCES_108_2_699C23","doi-asserted-by":"publisher","first-page":"11039","DOI":"10.1073\/pnas.90.23.11039","article-title":"Molecular cloning and centrosomal localization of human caltractin","volume":"90","author":"Lee","year":"1993","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"2023122400553433100_JOCES_108_2_699C24","first-page":"154","article-title":"Direct protein microsequencing from Immobilon-P transfer membrane","volume":"6","author":"LeGendre","year":"1988","journal-title":"Biotechniques"},{"key":"2023122400553433100_JOCES_108_2_699C25","doi-asserted-by":"publisher","first-page":"2153","DOI":"10.1101\/gad.5.12a.2153","article-title":"Polo encodes a protein-kinase homolog required for mitosis in Drosophila","volume":"5","author":"L\u2019Lamazares","year":"1991","journal-title":"Gene Dev"},{"key":"2023122400553433100_JOCES_108_2_699C26","first-page":"255","article-title":"Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells","volume":"54","author":"Maekawa","year":"1991","journal-title":"Eur. J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C27","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/0092-8674(91)90524-3","article-title":"Mircosurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cells","volume":"67","author":"Maniotis","year":"1991","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C28","doi-asserted-by":"crossref","first-page":"5737","DOI":"10.1016\/S0021-9258(19)49597-5","article-title":"The bimB3 mutation of Aspergillus nidulans uncouples DNA-replication from the completion of mitosis","volume":"267","author":"May","year":"1992","journal-title":"J. Biol. Chem"},{"key":"2023122400553433100_JOCES_108_2_699C29","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/S0074-7696(08)61698-8","article-title":"The chromosome cycle and the centrosome cycle in the mitotic cycle","volume":"100","author":"Mazia","year":"1987","journal-title":"Int. Rev. Cytol"},{"key":"2023122400553433100_JOCES_108_2_699C30","doi-asserted-by":"publisher","first-page":"1443","DOI":"10.1091\/mbc.3.12.1443","article-title":"Requirement for esp1 in the nuclear division of Saccharomyces cerevisiae","volume":"3","author":"McGrew","year":"1992","journal-title":"Mol. Biol. Cell"},{"key":"2023122400553433100_JOCES_108_2_699C31","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1038\/338662a0","article-title":"Identification of \u03b3-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans","volume":"338","author":"Oakley","year":"1989","journal-title":"Nature"},{"key":"2023122400553433100_JOCES_108_2_699C32","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1016\/0092-8674(90)90693-9","article-title":"\u03b3-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans","volume":"61","author":"Oakley","year":"1990","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C33","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/0378-1119(90)90056-W","article-title":"Phage \u03bbcDNA cloning vectors for subtractive hybridization, fusion-protein sunthesis and Cre-los P automatic plasmid subcloning","volume":"88","author":"Palazzolo","year":"1990","journal-title":"Gene"},{"key":"2023122400553433100_JOCES_108_2_699C34","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/0012-1606(88)90273-4","article-title":"Germinal vesicle components are not required for the cell-cycle oscillator of the early starfish embryo","volume":"128","author":"Picard","year":"1988","journal-title":"Dev. Biol"},{"key":"2023122400553433100_JOCES_108_2_699C35","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1083\/jcb.107.6.2009","article-title":"Nuclear and cytoplasmic mitotic-cycles continue in Drosophila embryos in which DNA-synthesis is inhibited with aphidicolin","volume":"107","author":"Raff","year":"1988","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C36","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/B978-0-12-394770-3.50007-3","article-title":"Ultrastructure of centrosome domains and identification of their protein components","volume-title":"In The Centrosome","author":"Rattner","year":"1992"},{"key":"2023122400553433100_JOCES_108_2_699C37","first-page":"117","article-title":"The centrosome cycle in PtK cells: asymmetric distribution and structural changes in pericentriolar material","volume":"44","author":"Rieder","year":"1982","journal-title":"Biol. Cell"},{"key":"2023122400553433100_JOCES_108_2_699C38","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1083\/jcb.21.3.429","article-title":"The ultrastructure of a mammalian cell during the mitotic cycle","volume":"21","author":"Robbins","year":"1964","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C39","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1083\/jcb.36.2.329","article-title":"The centriole cycle in synchronized HeLa cells","volume":"36","author":"Robbins","year":"1968","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C40","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/0092-8674(87)90712-4","article-title":"Kar1, a gene required for function of both intranuclear and extranuclear microtubules in yeast","volume":"48","author":"Rose","year":"1987","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C41","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"2023122400553433100_JOCES_108_2_699C42","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1093\/oxfordjournals.jhered.a109225","article-title":"Influence of the bn gene on mitosis of immature red blood cells in turkeys","volume":"70","author":"Searle","year":"1979","journal-title":"J. Heredity"},{"key":"2023122400553433100_JOCES_108_2_699C43","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1242\/jcs.89.1.57","article-title":"Distribution of pericentriolar material in multipolar spindles induced by colcemid treatment in Chinese hamster ovary cells","volume":"89","author":"Sellitto","year":"1988","journal-title":"J. Cell Sci"},{"key":"2023122400553433100_JOCES_108_2_699C44","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1083\/jcb.110.6.2025","article-title":"Protein-synthesis and the cell-cycle: centrosome reproduction in sea-urchin eggs is not under translational control","volume":"110","author":"Sluder","year":"1990","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C45","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/0092-8674(91)90390-K","article-title":"\u03b3-tubulin is a highly conserved component of the centrosome","volume":"65","author":"Stearns","year":"1991","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C46","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/0092-8674(94)90503-7","article-title":"In vitro reconstitution of centrosome assembly and function: the central role of \u03b3-tubulin","volume":"76","author":"Stearns","year":"1994","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C47","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1242\/jcs.89.1.25","article-title":"Polo, a mitotic mutant of Drosophila displaying abnormal spindle poles","volume":"89","author":"Sunkel","year":"1988","journal-title":"J. Cell Sci"},{"key":"2023122400553433100_JOCES_108_2_699C48","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/0092-8674(90)90266-H","article-title":"The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast esp1 gene","volume":"62","author":"Uzawa","year":"1990","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C49","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1002\/jcp.1041540302","article-title":"Heat shock alters centrosome organization leading to mitotic dysfunction and cell death","volume":"154","author":"Vidair","year":"1993","journal-title":"J. Cell. Physiol"},{"key":"2023122400553433100_JOCES_108_2_699C50","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1083\/jcb.114.4.745","article-title":"Mps1 and mps2, novel yeast genes defining distinct steps of spindle pole body duplication","volume":"114","author":"Winey","year":"1991","journal-title":"J. Cell Biol"},{"key":"2023122400553433100_JOCES_108_2_699C51","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1016\/0092-8674(91)90389-G","article-title":"\u03b3-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome","volume":"65","author":"Zheng","year":"1991","journal-title":"Cell"},{"key":"2023122400553433100_JOCES_108_2_699C52","doi-asserted-by":"publisher","first-page":"00","DOI":"10.1242\/jcs.108.2.685","article-title":"Identification of a novel Ca2+-regulated protein that is associated with the marginal band and centrosomes of chicken erythrocytes","volume":"108","author":"Zhu","year":"1995","journal-title":"J. Cell Sci"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/108\/2\/699\/3324408\/joces_108_2_699.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/108\/2\/699\/3324408\/joces_108_2_699.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T00:55:56Z","timestamp":1703379356000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/108\/2\/699\/24422\/Novel-centrosomal-protein-reveals-the-presence-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2,1]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,2,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.108.2.699","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2,1]]}}}