{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:42:56Z","timestamp":1767861776061,"version":"3.49.0"},"reference-count":46,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The effects of estrogen (E2) and progesterone (P) were examined on the expression levels of multiple gap junction (GJ) gene products (\u03b11=Cx43, \u03b21=Cx32, \u03b22=Cx26) in the uterus and ovaries of immature rats by immunohisto- chemistry, electron microscopy and northern blot analysis. E2 induced the expression of \u03b11 connexin in the uterus (specifically in the myometrium and in endometrial stroma proximal to luminal epithelium) and ovaries. The E2- induced \u03b11 expression was completely suppressed by P in the uterus, but only partly in ovaries. Steroid hormones also modulated the quantity, size, and distribution of \u03b21 and \u03b22 containing junctional plaques along lateral cell borders in polarized luminal and glandular uterine epithelia. Small GJs were detected at basolateral regions in proliferative luminal epithelium following administration of E2. In contrast, large GJs were localized at subapical- lateral cell borders of the secretory epithelium following P- treatment. The co-administration of E2+P had a synergis- tic effect on \u03b21 and \u03b22 expression in the luminal epithelium, but an inhibitory effect on \u03b22 expression in glandular epithelium. Myometrial GJs were detected in freeze- fracture replicas as aggregates containing regularly arranged particles with particle free zones. In contrast, GJs in secretory epithelium contained particles which were arranged in a non-crystalline fashion. These GJs contained domains of mixed and segregated \u03b21 and \u03b22 antigens within a single plaque as revealed by laser scanning confocal microscopy analysis of immuno-double-labeled secretory epithelium. The demonstration of segregated antigens within a single GJ plaque indicates the possibility of multiple channel populations formed by homo-oligomeric connexons. These results suggest that different connexins can be differentially regulated by steroid hormones in different cell types, and that the same steroid hormone can have different effects on the same connexin in different cell types.<\/jats:p>","DOI":"10.1242\/jcs.108.3.1017","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:14:55Z","timestamp":1619367295000},"page":"1017-1032","source":"Crossref","is-referenced-by-count":67,"title":["Gap junction regulation in the uterus and ovaries of immature rats by estrogen and progesterone"],"prefix":"10.1242","volume":"108","author":[{"given":"Boris","family":"Risek","sequence":"first","affiliation":[{"name":"1 Department of Cell Biology, 10 666 N. Torrey Pines Road, The Scripps Research Institute, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. George","family":"Klier","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology, 10 666 N. Torrey Pines Road, The Scripps Research Institute, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Phillips","sequence":"additional","affiliation":[{"name":"2 R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. W.","family":"Hahn","sequence":"additional","affiliation":[{"name":"2 R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. B.","family":"Gilula","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology, 10 666 N. 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