{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:51:55Z","timestamp":1773658315719,"version":"3.50.1"},"reference-count":52,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1995,4,1]],"date-time":"1995-04-01T00:00:00Z","timestamp":796694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>This report provides evidence for the formation of a cell surface-associated centrosome with two spatially discrete microtubule-nucleating sites that perform differently; the minus ends of microtubules remain anchored to one site but escape from the other. Centrosomal reorganization in the cells in question, outer pillar cells of the organ of Corti, indicates that its pericentriolar material becomes intimately associated with the plasma membrane at the two nucleating sites.<\/jats:p>\n               <jats:p>Two large microtubule bundles assemble in each cell. A beam which includes about 1,300 microtubules spans most of the cell apex. It is positioned at right angles to a pillar with about 4,500 microtubules which is oriented parallel to the cell\u2019s longitudinal axis.<\/jats:p>\n               <jats:p>The beam\u2019s microtubules elongate from, and remain attached to, a centrosomal region with two centrioles which acts as a microtubule-nucleating site. However, the elon-gating microtubules do not radiate from the immediate vicinity of the centrioles. During beam assembly, the minus ends of the microtubules are concentrated together close to the plasma membrane (less than 0.2 \u03bcm away in many cases) at a site which is located to one side of the cell apex. High concentrations of the pillar\u2019s microtubules elongating from one particular site have not been detected. Analyses of pillar assembly indicate that the following sequence of events occurs. Pillar microtubules elongate from an apical cell surface-associated nucleating site, which becomes more distantly separated from the centriolar locality as cell morphogenesis progresses. Microtubules do not accumulate at this apical nucleating site because they escape from it. They migrate down to lower levels in the cell where the mature bundle is finally situated and their plus ends are captured at the cell base.<\/jats:p>","DOI":"10.1242\/jcs.108.4.1333","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:18:56Z","timestamp":1619367536000},"page":"1333-1345","source":"Crossref","is-referenced-by-count":22,"title":["Formation of two microtubule-nucleating sites which perform differently during centrosomal reorganization in a mouse cochlear epithelial cell"],"prefix":"10.1242","volume":"108","author":[{"given":"John B.","family":"Tucker","sequence":"first","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette M.","family":"Mogensen","sequence":"additional","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calum C.","family":"Paton","sequence":"additional","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John B.","family":"Mackie","sequence":"additional","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig G.","family":"Henderson","sequence":"additional","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorna M.","family":"Leckie","sequence":"additional","affiliation":[{"name":"University of St Andrews School of Biological and Medical Sciences, Bute Medical Building , , St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,4,1]]},"reference":[{"key":"2023122401150398600_JOCES_108_4_1333C1","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1083\/jcb.109.1.179","article-title":"Role of microtubules in polarized delivery of apical membrane proteins to the brush border of the intestinal epithelium","volume":"109","author":"Achler","year":"1989","journal-title":"J. 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