{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T10:42:00Z","timestamp":1770460920090,"version":"3.49.0"},"reference-count":39,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1995,4,1]],"date-time":"1995-04-01T00:00:00Z","timestamp":796694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The epidermal blistering desease, pemphigus vulgaris (PV), is caused by circulating autoantibodies that react with a desmosomal glycoprotein desmoglein (Dsg3). This antigen is expressed only in stratified epithelial tissues. Here we show that the simple epithelial canine kidney cell line, MDCK, expresses at least two desmoglein isoforms recognised by different monoclonal antibodies. One of these isoforms is a 130\u00d7103Mr polypeptide that is recognised by both PV autoantisera and a monoclonal antibody reactive with a cytoplasmic domain of human Dsg3. Antibodies in PV sera bind to the surface of MDCK cells but do not cause loss of intercellular adhesion. This is the first demonstration of the expression of a polypeptide related to human PV antigen by a simple epithelial cell type.<\/jats:p>","DOI":"10.1242\/jcs.108.4.1743","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:26:10Z","timestamp":1619367970000},"page":"1743-1750","source":"Crossref","is-referenced-by-count":21,"title":["A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen"],"prefix":"10.1242","volume":"108","author":[{"given":"Marcelo","family":"Jos\u00e9 Vilela","sequence":"first","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa 1 Departamento de Biologi\u00e0 Animal , , 36570 Vi\u00e7osa, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Keio University School of Medicine 2 Department of Dermatology , , 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeji","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Keio University School of Medicine 2 Department of Dermatology , , 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison J.","family":"North","sequence":"additional","affiliation":[{"name":"University of Manchester 3 Cancer Research Campaign Epithelial Morphogenesis Research Group, School of Biological Sciences , , 3.239 Stopford Building, Oxford Road, Manchester M13 9PT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Garrod","sequence":"additional","affiliation":[{"name":"University of Manchester 3 Cancer Research Campaign Epithelial Morphogenesis Research Group, School of Biological Sciences , , 3.239 Stopford Building, Oxford Road, Manchester M13 9PT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,4,1]]},"reference":[{"key":"2023122401140782100_JOCES_108_4_1743C1","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/0092-8674(91)90360-B","article-title":"Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion","volume":"67","author":"Amagai","year":"1991","journal-title":"Cell"},{"key":"2023122401140782100_JOCES_108_4_1743C2","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1242\/jcs.104.3.741","article-title":"Stratification related expression of isoforms of the desmosomal cadherins in human epidermis","volume":"104","author":"Arnemann","year":"1993","journal-title":"J. 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