{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:46:53Z","timestamp":1770493613446,"version":"3.49.0"},"reference-count":47,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[1996,7,1]],"date-time":"1996-07-01T00:00:00Z","timestamp":836179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In different systems, cAMP either blocks or promotes cell cycle progression in mid to late G1 phase. Dog thyroid epithelial cells in primary culture constitute a model of positive control of DNA synthesis initiation and G0-S pre-replicative phase progression by cyclic AMP (cAMP) as a second messenger for thyrotropin (TSH). We report here that TSH markedly increases the expression of p27kip1, the inhibitor of the cell cycle and cyclin-dependent kinases. This effect was prevented by the concomitant administration of the cAMP-independent mitogens, epidermal growth factor (EGF)+serum. EGF+serum also slightly inhibited the weak basal accumulation of p27kip1. Nevertheless, in the case of stimulation by TSH alone, the cAMP-dependent cell cycle progression was fully compatible with the enhanced expression of p27kip1. This observation is paradoxical since a decrease of p27kip1 is generally associated with growth stimulation in other systems, and since a similar cAMP-dependent increase of p27kip1 in macrophages has been found responsible for mid-G1 cell cycle arrest. The opposite regulation of p27kip1 in response to TSH or EGF+serum in dog thyroid epithelial cells suggests a major difference at mid to late G1 stages between cAMP-dependent and cAMP-independent mitogenic pathways.<\/jats:p>","DOI":"10.1242\/jcs.109.7.1759","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:38:29Z","timestamp":1619368709000},"page":"1759-1764","source":"Crossref","is-referenced-by-count":26,"title":["Paradoxical accumulation of the cyclin-dependent kinase inhibitor p27<i>kip1<\/i> during the cAMP-dependent mitogenic stimulation of thyroid epithelial cells"],"prefix":"10.1242","volume":"109","author":[{"given":"Fabienne","family":"Depoortere","sequence":"first","affiliation":[{"name":"Institute of Interdisciplinary Research, Universit\u00e9 Libre de Bruxelles, Campus Erasme, B-1070 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques E.","family":"Dumont","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Research, Universit\u00e9 Libre de Bruxelles, Campus Erasme, B-1070 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre P.","family":"Roger","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Research, Universit\u00e9 Libre de Bruxelles, Campus Erasme, B-1070 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1996,7,1]]},"reference":[{"key":"2024010323543539400_JOCES_109_7_1759C1","doi-asserted-by":"crossref","first-page":"18195","DOI":"10.1074\/jbc.270.31.18195","article-title":"Both p16 and p21 families of cyclin-dependent kinase (CDK) inhibitors block phosphorylation of cyclin-dependent kinases by the CDK-activating kinase","volume":"270","author":"Aprelikova","year":"1995","journal-title":"J. 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