{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:49:24Z","timestamp":1791323364197,"version":"4.3.1"},"reference-count":40,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1997,1,1]],"date-time":"1997-01-01T00:00:00Z","timestamp":852076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In vivo, normal mammary epithelial cells utilize hemidesmosome attachment devices to adhere to stroma. However, analyses of a potential role for hemidesmosomes and their components in mammary epithelial tissue morphogenesis have never been attempted. MCF-10A cells are a spontaneously immortalized line derived from mammary epithelium and possess a number of characteristics of normal mammary epithelial cells including expression of hemidesmosomal associated proteins such as the two bullous pemphigoid antigens, \u03b16\u03b24 integrin and its ligand laminin-5. More importantly, MCF-10A cells readily assemble mature hemidesmosomes when plated onto uncoated substrates. When maintained on matrigel, like their normal breast epithelial cell counterparts, MCF-10A cells undergo a branching morphogenesis and assemble hemidesmosomes at sites of cell-matrigel interaction. Function blocking antibodies specific for human laminin-5 and the \u03b1 subunits of its two known receptors (\u03b13\u03b21 and \u03b16\u03b24 integrin) not only inhibit hemidesmosome assembly by MCF-10A cells but also impede branching morphogenesis induced by matrigel. Our results imply that the hemidesmosome, in particular those subunits comprising its laminin-5\/integrin \u2018backbone\u2019, play an important role in morphogenetic events. We discuss these results in light of recent evidence that hemidesmosomes are sites involved in signal transduction.<\/jats:p>","DOI":"10.1242\/jcs.110.1.55","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:38:42Z","timestamp":1619368722000},"page":"55-63","source":"Crossref","is-referenced-by-count":94,"title":["The role of laminin-5 and its receptors in mammary epithelial cell branching morphogenesis"],"prefix":"10.1242","volume":"110","author":[{"given":"Stephanie","family":"Stahl","sequence":"first","affiliation":[{"name":"Molecular Biology Northwestern University Medical School, Chicago 1 Departments of Cell , , IL, USA"},{"name":"Northwestern University Medical School, Chicago 2 Medicine , , IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigmund","family":"Weitzman","sequence":"additional","affiliation":[{"name":"Northwestern University Medical School, Chicago 2 Medicine , , IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan C. 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