{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:48:43Z","timestamp":1791406123396,"version":"4.3.3"},"reference-count":29,"publisher":"The Company of Biologists","issue":"14","license":[{"start":{"date-parts":[[1997,7,15]],"date-time":"1997-07-15T00:00:00Z","timestamp":868924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The emergence of the mammalian blastocysts from their thick glycoprotein investment known as the zona pellucida is an important, but poorly understood, event in embryogenesis. In this paper, we demonstrate that peri-hatching blastocysts generate a considerably high quantum of an active oxyradical species for an extremely short period of time when compared to the pre-hatching (unhatched) and post-hatching (hatched) blastocysts. Hatching could be induced in pre-hatching blastocysts by exposing them to superoxide artificially generated to match the observed peri-implantation stage specific levels of superoxide, without impairing their viability. These observations suggest the operation of a superoxide-dependent hatching initiation in developing mammalian embryos.<\/jats:p>","DOI":"10.1242\/jcs.110.14.1597","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:46:48Z","timestamp":1619369208000},"page":"1597-1602","source":"Crossref","is-referenced-by-count":42,"title":["A programmed oxyradical burst causes hatching of mouse blastocysts"],"prefix":"10.1242","volume":"110","author":[{"given":"M.","family":"Thomas","sequence":"first","affiliation":[{"name":"Devi Ahilya University, Vigyan Bhawan School of Life Sciences , , Khandwa Road, Indore 452 001, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Jain","sequence":"additional","affiliation":[{"name":"Devi Ahilya University, Vigyan Bhawan School of Life Sciences , , Khandwa Road, Indore 452 001, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. P.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Devi Ahilya University, Vigyan Bhawan School of Life Sciences , , Khandwa Road, Indore 452 001, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Laloraya","sequence":"additional","affiliation":[{"name":"Devi Ahilya University, Vigyan Bhawan School of Life Sciences , , Khandwa Road, Indore 452 001, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1997,7,15]]},"reference":[{"key":"2024010400343133100_JOCES_110_14_1597C1","first-page":"15","article-title":"Pre-implantation development of the mammalian embryo","volume":"29","author":"Agostoni","year":"1993","journal-title":"Ann. dell Insti. Superiore di Sanita"},{"key":"2024010400343133100_JOCES_110_14_1597C2","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1002\/jez.1402630414","article-title":"Micromanipulation of cleaved embryos cultured in protein-free medium: a mouse model for assisted hatching","volume":"263","author":"Alikani","year":"1992","journal-title":"J. Exp. Zool"},{"key":"2024010400343133100_JOCES_110_14_1597C3","first-page":"177","article-title":"The development of trophoblast in vitro from blastocysts containing varying amounts of inner cell mass","volume":"33","author":"Ansell","year":"1975","journal-title":"J. Embryol. Exp. Morphol"},{"key":"2024010400343133100_JOCES_110_14_1597C4","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1002\/mrd.1120140310","article-title":"Estimation and manipulation of glutathione levels in prepuberal mouse ovaries and ova: relevance to sperm nucleus transformation in the fertilized egg","volume":"14","author":"Calvin","year":"1986","journal-title":"Gamete Res"},{"key":"2024010400343133100_JOCES_110_14_1597C5","first-page":"481","article-title":"Cinematographic observations of the trophoblast and zona pellucida of the mouse blastocyst","volume":"17","author":"Cole","year":"1967","journal-title":"J. Embryol. Exp. Morphol"},{"key":"2024010400343133100_JOCES_110_14_1597C6","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1002\/mrd.1080320407","article-title":"Changes in cAMP phosphodiesterase activity and cAMP concentration during mouse pre-implantation development","volume":"32","author":"Dardik","year":"1992","journal-title":"Mol. Reprod. Dev"},{"key":"2024010400343133100_JOCES_110_14_1597C7","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1242\/dev.120.5.1071","article-title":"Heparin-binding EGF-like growth factor gene is induced in the mouse uterus temporally by the blastocyst solely at the site of its apposition: a possible ligand for interaction with blastocyst EGF-receptor in implantation","volume":"120","author":"Das","year":"1994","journal-title":"Development"},{"key":"2024010400343133100_JOCES_110_14_1597C8","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1002\/mrd.1120150203","article-title":"Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing the hardening of the zona pellucida","volume":"15","author":"Downs","year":"1986","journal-title":"Gamete Res"},{"key":"2024010400343133100_JOCES_110_14_1597C9","first-page":"139","volume-title":"In Mechanism and Control of Animal Fertilization","author":"Dunbar","year":"1983"},{"key":"2024010400343133100_JOCES_110_14_1597C10","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1095\/biolreprod51.6.1307","article-title":"Status of glutathione during oxidant-induced oxidative stress in the pre-implantation mouse embryo","volume":"51","author":"Gardiner","year":"1994","journal-title":"Biol. Reprod"},{"key":"2024010400343133100_JOCES_110_14_1597C11","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1006\/abbi.1995.1361","article-title":"Glutathione redox cycle-driven recovery of reduced glutathione after oxidation by tertiary-butyl hydroperoxide in pre-implantation mouse embryos","volume":"321","author":"Gardiner","year":"1995","journal-title":"Arch. Biochem. Biophys"},{"key":"2024010400343133100_JOCES_110_14_1597C12","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1095\/biolreprod48.2.377","article-title":"Amino acid and ammonium regulate mouse embryo development in culture","volume":"48","author":"Gardner","year":"1993","journal-title":"Biol. Reprod"},{"key":"2024010400343133100_JOCES_110_14_1597C13","doi-asserted-by":"publisher","first-page":"1649","DOI":"10.1126\/science.275.5306.1649","article-title":"Mitogenic signaling mediated by oxidants in Ras-transformed fibroblasts","volume":"275","author":"Irani","year":"1997","journal-title":"Science"},{"key":"2024010400343133100_JOCES_110_14_1597C14","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1530\/jrf.0.0680471","article-title":"Evidence that protease action is not specifically involved in the hatching of rabbit blastocysts caused by commercial bovine serum albumin in culture","volume":"68","author":"Kane","year":"1983","journal-title":"J. Reprod. Fertil"},{"key":"2024010400343133100_JOCES_110_14_1597C15","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1530\/jrf.0.0780225","article-title":"A survey of the effects of proteases and glycosidases on culture of rabbit morulae to blastocysts","volume":"78","author":"Kane","year":"1986","journal-title":"J. Reprod. Fertil"},{"key":"2024010400343133100_JOCES_110_14_1597C16","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1002\/jez.1402470211","article-title":"Protein-free culture medium containing polyvinyl alcohol, vitamins and amino acids supports development of eight-cell hamster embryos to hatching blastocysts","volume":"247","author":"Kane","year":"1988","journal-title":"J. Exp. Zool"},{"key":"2024010400343133100_JOCES_110_14_1597C17","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1093\/oxfordjournals.humrep.a137685","article-title":"Cruciate thinning of the zona pellucida for more successful enhancement of blastocyst hatching in the mouse","volume":"7","author":"Khalifa","year":"1992","journal-title":"Hum. Reprod"},{"key":"2024010400343133100_JOCES_110_14_1597C18","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/S0006-291X(88)80025-1","article-title":"Changes in the levels of superoxide anion radical and superoxide dismutase activity during the estrus cycle of Rattus norvegicus and induction of superoxide dismutase in rat ovary by lutropin","volume":"157","author":"Laloraya","year":"1988","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"2024010400343133100_JOCES_110_14_1597C19","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1111\/j.1432-1033.1974.tb03714.x","article-title":"Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay of superoxide dismutase","volume":"47","author":"Marklund","year":"1974","journal-title":"Eur. J. Biochem"},{"key":"2024010400343133100_JOCES_110_14_1597C20","first-page":"153","article-title":"A role for cytokines in early pregnancy","volume":"38","author":"Mathilagan","year":"1994","journal-title":"Ind. J. Physiol. Pharmacol"},{"key":"2024010400343133100_JOCES_110_14_1597C21","first-page":"1","article-title":"The fate of the zona pellucida in mice","volume":"23","author":"McLaren","year":"1970","journal-title":"J. Embryol. Exp. Morphol"},{"key":"2024010400343133100_JOCES_110_14_1597C22","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1126\/science.138.3540.594","article-title":"Experimental study of the developing mammalian egg: Removal of the zona pellucida","volume":"138","author":"Mintz","year":"1962","journal-title":"Science"},{"key":"2024010400343133100_JOCES_110_14_1597C23","first-page":"539","article-title":"The role of zona pellucida in the development of mouse eggs in vivo","volume":"23","author":"Modlinski","year":"1970","journal-title":"J. Embryol. Exp. Morph"},{"key":"2024010400343133100_JOCES_110_14_1597C24","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/0012-1606(88)90070-X","article-title":"Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing capacity in maturing hamster oocytes","volume":"125","author":"Perreault","year":"1988","journal-title":"Dev. Biol"},{"key":"2024010400343133100_JOCES_110_14_1597C25","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1111\/j.1432-0436.1991.tb00880.x","article-title":"Hydrogen peroxide as a mediator of programmed cell death in the blastocyst","volume":"46","author":"Pierce","year":"1991","journal-title":"Differentiation"},{"key":"2024010400343133100_JOCES_110_14_1597C26","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/S0015-0282(16)57357-9","article-title":"Physiological characterization of blastocyst hatching mechanism by use of a mouse anti-hatching model","volume":"63","author":"Schiew","year":"1995","journal-title":"Fertil. Steril"},{"key":"2024010400343133100_JOCES_110_14_1597C27","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1002\/jez.1401860308","article-title":"Hatching, attachment and outgrowth of mouse blastocysts in vitro: fixed nitrogen requirements","volume":"186","author":"Spindle","year":"1973","journal-title":"J. Exp. Zool"},{"key":"2024010400343133100_JOCES_110_14_1597C28","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/0014-4827(82)90381-0","article-title":"Sensitivity of early mouse embryos to [3H]-thymidine","volume":"142","author":"Spindle","year":"1982","journal-title":"Exp. Cell Res"},{"key":"2024010400343133100_JOCES_110_14_1597C29","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1095\/biolreprod49.1.89","article-title":"Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus","volume":"49","author":"Yoshida","year":"1993","journal-title":"Biol. Reprod"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/110\/14\/1597\/3335476\/joces_110_14_1597.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/110\/14\/1597\/3335476\/joces_110_14_1597.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:34:55Z","timestamp":1704328495000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/110\/14\/1597\/25039\/A-programmed-oxyradical-burst-causes-hatching-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,15]]},"references-count":29,"journal-issue":{"issue":"14","published-print":{"date-parts":[[1997,7,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.110.14.1597","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,15]]}}}