{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:38:23Z","timestamp":1777430303259,"version":"3.51.4"},"reference-count":41,"publisher":"The Company of Biologists","issue":"20","license":[{"start":{"date-parts":[[1997,10,15]],"date-time":"1997-10-15T00:00:00Z","timestamp":876873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Retinitis pigmentosa is a heterogeneous group of inherited retinal disorders in which the photoreceptor cells degenerate. A line of transgenic mice expresses a mutant opsin gene that encodes three missense mutations near the amino terminus, including P23H, which is the basis for a common form of dominant retinitis pigmentosa. By studying the photoreceptor cells of these mice and their normal littermates, we found that: (1) opsin was routed correctly, (2) the concentration of opsin in the disk membranes appeared normal by freeze fracture analysis, (3) the amount of disk membrane shedding was normal, but (4) the basal disks of the outer segments were disorganized, indicating defective disk membrane morphogenesis. Defective disk membrane morphogenesis appears to result in the formation of fewer mature disks, thus accounting for observed gradual shortening of the photoreceptor outer segments with age. We suggest that abnormal disk membrane morphogenesis is the primary cellular defect that leads to blindness, and that it arises from the inability of nascent disk membranes, containing normal and mutant opsin, to interact normally with each other.<\/jats:p>","DOI":"10.1242\/jcs.110.20.2589","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T16:47:34Z","timestamp":1619369254000},"page":"2589-2597","source":"Crossref","is-referenced-by-count":36,"title":["Defective phototransductive disk membrane morphogenesis in transgenic mice expressing opsin with a mutated N-terminal domain"],"prefix":"10.1242","volume":"110","author":[{"given":"Xinran","family":"Liu","sequence":"first","affiliation":[{"name":"1 Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, California 92093-0983, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting-Huai","family":"Wu","sequence":"additional","affiliation":[{"name":"University of Illinois, Chicago 2 Department of Ophthalmology and Visual Science , , Illinois 60612, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally","family":"Stowe","sequence":"additional","affiliation":[{"name":"Australian National University, Canberra 3 Research School of Biological Sciences , , ACT 2601, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuko","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Yokohama City University, 22-2 Seto 4 Department of Biology , , Kanazawa-ku, Yokohama 236, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Arikawa","sequence":"additional","affiliation":[{"name":"Yokohama City University, 22-2 Seto 4 Department of Biology , , Kanazawa-ku, Yokohama 236, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muna I.","family":"Naash","sequence":"additional","affiliation":[{"name":"University of Illinois, Chicago 2 Department of Ophthalmology and Visual Science , , Illinois 60612, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Williams","sequence":"additional","affiliation":[{"name":"1 Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, California 92093-0983, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1997,10,15]]},"reference":[{"key":"2024010621441318400_JOCES_110_20_2589C1","doi-asserted-by":"publisher","first-page":"20563","DOI":"10.1016\/S0021-9258(17)30539-2","article-title":"Mouse opsin. 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