{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:07:12Z","timestamp":1791148032577,"version":"4.1.0"},"reference-count":47,"publisher":"The Company of Biologists","issue":"17","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>An immortalized implantation site intermediate trophoblastic cell line, IST-1, was established from a human placenta of 7 weeks gestation. IST-1 cells phenotypically resembled the implantation site intermediate trophoblastic cells in situ and expressed Mel-CAM (MUC 18 or CD146). Mel-CAM is a cell adhesion molecule belonging to the immunoglobulin gene superfamily. It is involved in heterophilic cell-cell adhesion and plays a role in several biological processes including tumor progression. We have previously shown that Mel-CAM was highly expressed in the intermediate (extravillous) trophoblast in the human implantation site. In this study we determined the function of Mel-CAM in the interaction of trophoblast and uterine smooth muscle in the implantation site. IST-1 cells failed to adhere to immobilized recombinant Mel-CAM in solid phase whereas the uterine smooth muscle cells did. The presence of the putative Mel-CAM ligand in smooth muscle cells was further supported by the finding that Mel-CAM-transfected but not the mock-transfected U937 leukemia cells bind to the confluent monolayer of uterine smooth muscle cells. IST-1 cells attached efficiently to the monolayer of the uterine smooth muscle cells and acquired a spindle-shaped morphology simulating smooth muscle cells. The cell binding was only marginally affected by Mel-CAM blocking antibodies. However, Mel-CAM blocking antibodies and recombinant Mel-CAM promoted cell migration from IST-1 cell spheroids on the smooth muscle monolayer. Taken together, our results suggest that IST-1 cells express Mel-CAM but not the putative Mel-CAM ligand. In contrast, the uterine smooth muscle cells express the putative Mel-CAM ligand which binds to Mel-CAM on the surface of the IST-1 cells. The interaction between Mel-CAM and its putative ligand confers a stationary phenotype for trophoblastic cells. These observations are consistent with an important role for Mel-CAM in limiting trophoblastic migration within the myometrium in the implantation site.<\/jats:p>","DOI":"10.1242\/jcs.111.17.2655","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T17:05:09Z","timestamp":1619370309000},"page":"2655-2664","source":"Crossref","is-referenced-by-count":51,"title":["Expression of Mel-CAM in implantation site intermediate trophoblastic cell line, IST-1, limits its migration on uterine smooth muscle cells"],"prefix":"10.1242","volume":"111","author":[{"given":"I.-M.","family":"Shih","sequence":"first","affiliation":[{"name":"the Johns Hopkins University School of Medicine 1 Departments of Pathology , , Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.-L.","family":"Wang","sequence":"additional","affiliation":[{"name":"the Johns Hopkins University School of Medicine 1 Departments of Pathology , , Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.-C.","family":"Wu","sequence":"additional","affiliation":[{"name":"the Johns Hopkins University School of Medicine 1 Departments of Pathology , , Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Kurman","sequence":"additional","affiliation":[{"name":"the Johns Hopkins University School of Medicine 1 Departments of Pathology , , Baltimore, MD 21205, USA"},{"name":"the Johns Hopkins University School of Medicine 2 Gynecology & Obstetrics , , Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. D.","family":"Gearhart","sequence":"additional","affiliation":[{"name":"the Johns Hopkins University School of Medicine 2 Gynecology & Obstetrics , , Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1998,9,1]]},"reference":[{"key":"2024010700143850000_JOCES_111_17_2655C1","first-page":"191","article-title":"Simultaneous expression of 70 kilodalton type IV collagenase and type IV collagen 1(IV) chain genes by cells of early human placenta and gestational endometrium","volume":"67","author":"Autio-Harmainen","year":"1992","journal-title":"Lab. 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