{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:48:50Z","timestamp":1791460130021,"version":"4.3.4"},"reference-count":57,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[1998,12,18]],"date-time":"1998-12-18T00:00:00Z","timestamp":913939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,12,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>During Caenorhabditis elegans spermatogenesis, asymmetric partitioning of cellular components principally occurs via ER\/Golgi-derived organelles, named fibrous body-membranous organelles. In C. elegans spe-4 mutants, morphogenesis of fibrous body-membranous organelle complexes is defective and spermatogenesis arrests at an unusual cellular stage with four haploid nuclei within a common cytoplasm. The spe-4 encoded integral membrane protein is a diverged member of the presenilin family implicated in early onset Alzheimer\u2019s disease. Specific antisera were used to show that SPE-4 resides within the fibrous body-membranous organelles membranes during wild-type spermatogenesis. Several spe-4 recessive mutants were examined for SPE-4 immunoreactivity and a deletion mutant lacks detectable SPE-4 while either of two missense mutants synthesize and localize immunoreactive SPE-4 within their fibrous body-membranous organelles. One of these missense mutations is located within a motif that is common to all presenilins. spe-4 mutants were also examined for other partitioning defects and tubulin was found to accumulate in unusual deposits close to the plasma membrane. These results suggest that wild-type SPE-4 is required for proper localization of macromolecules that are subject to asymmetric partitioning during spermatogenesis.<\/jats:p>","DOI":"10.1242\/jcs.111.24.3645","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T17:03:47Z","timestamp":1619370227000},"page":"3645-3654","source":"Crossref","is-referenced-by-count":91,"title":["The presenilin protein family member SPE-4 localizes to an ER\/Golgi derived organelle and is required for proper cytoplasmic partitioning during <i>Caenorhabditis elegans<\/i> spermatogenesis"],"prefix":"10.1242","volume":"111","author":[{"given":"P. Michele","family":"Arduengo","sequence":"first","affiliation":[{"name":"Emory University, Atlanta, Georgia 30322 1 Graduate Program in Biochemistry , , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ollie Kelly","family":"Appleberry","sequence":"additional","affiliation":[{"name":"Emory University, Atlanta 2 Department of Biology, Cell and Developmental Biology , , Georgia 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peale","family":"Chuang","sequence":"additional","affiliation":[{"name":"Emory University, Atlanta 2 Department of Biology, Cell and Developmental Biology , , Georgia 30322, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W.","family":"L\u2019Hernault","sequence":"additional","affiliation":[{"name":"Emory University, Atlanta, Georgia 30322 1 Graduate Program in Biochemistry , , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1998,12,18]]},"reference":[{"key":"2024010700543841700_JOCES_111_24_3645C1","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucl. 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