{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:10:34Z","timestamp":1761664234408,"version":"3.37.0"},"reference-count":40,"publisher":"The Company of Biologists","issue":"19","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Presenilins were first identified as causative factors in early onset, familial Alzheimer\u2019s Disease (FAD). They are predicted to encode a highly conserved novel family of eight transmembrane domain proteins with a large hydrophilic loop between TM6 and TM7 that is the site of numerous FAD mutations. Here, we show that the loop region of Drosophila and human presenilins interacts with the C-terminal domain of Drosophila filamin. Furthermore, we show that Drosophila has at least two major filamin forms generated by alternative splicing from a gene that maps to position 89E10-89F4 on chromosome 3. The longest form is enriched in the central nervous system and ovaries, shares 41.7% overall amino acid identity with human filamin (ABP-280) and contains an N-terminal actin-binding domain. The shorter form is broadly expressed and encodes an alternatively spliced form of the protein lacking the actin-binding domain. Finally, we show that presenilin and filamin are expressed in overlapping patterns in Drosophila and that dominant adult phenotypes produced by overexpression of presenilin can be suppressed by overexpression of filamin in the same tissue. Taken together, these results suggest that presenilin and filamin functionally interact during development.<\/jats:p>","DOI":"10.1242\/jcs.113.19.3499","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T03:16:09Z","timestamp":1619234169000},"page":"3499-3508","source":"Crossref","is-referenced-by-count":26,"title":["Physical and genetic interaction of filamin with presenilin in <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"113","author":[{"given":"Yiquan","family":"Guo","sequence":"first","affiliation":[{"name":"555 University Avenue, Toronto 1 Program in Developmental Biology, The Hospital for Sick Children , , Ontario, Canada M5G 1X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"555 University Avenue, Toronto 1 Program in Developmental Biology, The Hospital for Sick Children , , Ontario, Canada M5G 1X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Sokol","sequence":"additional","affiliation":[{"name":"2 Departments of Genetics and Cell Biology, Yale Medical School, 333 Cedar Street, PO Box 208005, New Haven, CT 06520-8005, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn","family":"Cooley","sequence":"additional","affiliation":[{"name":"2 Departments of Genetics and Cell Biology, Yale Medical School, 333 Cedar Street, PO Box 208005, New Haven, CT 06520-8005, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle L.","family":"Boulianne","sequence":"additional","affiliation":[{"name":"555 University Avenue, Toronto 1 Program in Developmental Biology, The Hospital for Sick Children , , Ontario, Canada M5G 1X8"},{"name":"University of Toronto 3 Departments of Molecular and Medical Genetics and Zoology , , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2000,10,1]]},"reference":[{"key":"2025020707171684500_JOCES_113_19_3499C1","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1016\/S0896-6273(00)80230-5","article-title":"Familial Alzheimer\u2019s disease-linked presenilin 1 variants elevate A\u03b21-42\/1-40 ratio in vitro and in vivo","volume":"17","author":"Borchelt","year":"1996","journal-title":"Neuron"},{"key":"2025020707171684500_JOCES_113_19_3499C2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/nm0197-67","article-title":"Mutant presenilins of Alzheimer\u2019s disease increase production of 42-residue amyloid \u03b2-protein in both transfected cells and transgenic mice","volume":"3","author":"Citron","year":"1997","journal-title":"Nat. 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