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We present results to show that localised domains of elevated calcium were observed exclusively in the anterior dorsal part of the ectoderm, and that these transients increased in number and amplitude between stages 9 to 11, just prior to the onset of neural induction. During this time, however, no increase in cytosolic free calcium was observed in the ventral ectoderm, mesoderm or endoderm. The origin and role of these dorsal calcium-signalling patterns were also investigated. Calcium transients require the presence of functional L-type voltage-sensitive calcium channels. Inhibition of channel activation from stages 8 to 14 with the specific antagonist R(+)BayK 8644 led to a complete inhibition of the calcium transients during gastrulation and resulted in severe defects in the subsequent formation of the anterior nervous system. BayK treatment also led to a reduction in the expression of Zic3 and geminin in whole embryos, and of NCAM in noggin-treated animal caps. The possible role of calcium transients in regulating developmental gene expression is discussed.<\/jats:p>","DOI":"10.1242\/jcs.113.19.3519","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T03:16:43Z","timestamp":1619234203000},"page":"3519-3529","source":"Crossref","is-referenced-by-count":102,"title":["Imaging patterns of calcium transients during neural induction in <i>Xenopus laevis<\/i> embryos"],"prefix":"10.1242","volume":"113","author":[{"given":"Catherine","family":"Leclerc","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paul Sabatier, 118 Route de Narbonne 1 Centre de Biologie du D\u00e9veloppement, UMR 5547 , , F-31062 Toulouse CEDEX 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Webb","sequence":"additional","affiliation":[{"name":"Hong Kong University of Science and Technology 2 Department of Biology , , Clear Water Bay, Kowloon, Hong Kong, PRC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Daguzan","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paul Sabatier, 118 Route de Narbonne 1 Centre de Biologie du D\u00e9veloppement, UMR 5547 , , F-31062 Toulouse CEDEX 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Moreau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paul Sabatier, 118 Route de Narbonne 1 Centre de Biologie du D\u00e9veloppement, UMR 5547 , , F-31062 Toulouse CEDEX 04, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew L.","family":"Miller","sequence":"additional","affiliation":[{"name":"Hong Kong University of Science and Technology 2 Department of Biology , , Clear Water Bay, Kowloon, Hong Kong, PRC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,10,1]]},"reference":[{"key":"2025020707171564200_JOCES_113_19_3519C1","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1111\/j.1440-169X.1987.00535.x","article-title":"Competence as the main factor determining the size of the neural plate","volume":"29","author":"Albers","year":"1987","journal-title":"Dev. 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