{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:01:46Z","timestamp":1791291706543,"version":"4.1.0"},"reference-count":46,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2000,12,15]],"date-time":"2000-12-15T00:00:00Z","timestamp":976838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The PACSINs are a family of cytoplasmic phosphoproteins that play a role in vesicle formation and transport. We report the cloning and cDNA sequencing of PACSIN 3 and the analysis of all three PACSIN isoforms with regard to tissue distribution, ligand binding properties and influence on endocytosis. PACSIN 3 differs from the other family members in having a short proline-rich region and lacking asparagine-proline-phenylalanine motifs. In contrast to the neurospecific PACSIN 1 and the ubiquitously expressed PACSIN 2, PACSIN 3 is mainly detected in lung and muscle tissues. All isoforms potentially oligomerize and bind to dynamin, synaptojanin 1 and N-WASP via their Src homology 3 domains. The PACSIN proteins colocalize with dynamin, but not with clathrin, implying a specific role with a distinct subpopulation of dynamin at defined cellular sites. Transferrin endocytosis is blocked in a dose-dependent manner in cells overexpressing the PACSIN variants, but the inhibitory effect can be abolished by mutating specific amino acid residues in the Src homology 3 domains. These characteristics of the PACSIN protein family suggest a general function in recruitment of the interacting proteins to sites of endocytosis.<\/jats:p>","DOI":"10.1242\/jcs.113.24.4511","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T18:30:51Z","timestamp":1619375451000},"page":"4511-4521","source":"Crossref","is-referenced-by-count":183,"title":["All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis"],"prefix":"10.1242","volume":"113","author":[{"given":"Jan","family":"Modregger","sequence":"first","affiliation":[{"name":"Institute for Biochemistry II, University of Cologne 1 , D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Ritter","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, University of Cologne 1 , D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Witter","sequence":"additional","affiliation":[{"name":"Institute for Anatomy II, Medical Faculty, University of Cologne 2 , D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Paulsson","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, University of Cologne 1 , D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Plomann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry II, University of Cologne 1 , D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,12,15]]},"reference":[{"key":"2023101823044024300_JOCES_113_24_4511C1","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"2023101823044024300_JOCES_113_24_4511C2","doi-asserted-by":"publisher","first-page":"12111","DOI":"10.1074\/jbc.271.20.12111","article-title":"The ear of \u03b1-adaptin interacts with the COOH-terminal domain of the Eps15 protein","volume":"271","author":"Benmerah","year":"1996","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C3","doi-asserted-by":"publisher","first-page":"8259","DOI":"10.1073\/pnas.92.18.8259","article-title":"Predicting coiled coils by use of pairwise residue correlations","volume":"92","author":"Berger","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023101823044024300_JOCES_113_24_4511C4","first-page":"53","article-title":"A generalized profile syntax for biomolecular sequence motifs and its function in automatic sequence interpretation","volume":"2","author":"Bucher","year":"1994","journal-title":"Proc. 2nd Int. Conf. on Intelligent Systems for Molecular Biology"},{"key":"2023101823044024300_JOCES_113_24_4511C5","doi-asserted-by":"publisher","first-page":"2595","DOI":"10.1091\/mbc.9.9.2595","article-title":"Differential distribution of dynamin isoforms in mammalian cells","volume":"9","author":"Cao","year":"1998","journal-title":"Mol. Biol. Cell"},{"key":"2023101823044024300_JOCES_113_24_4511C6","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1046\/j.1471-4159.1996.67030927.x","article-title":"Three dynamin-encoding genes are differentially expressed in developing rat brain","volume":"67","author":"Cook","year":"1996","journal-title":"J. Neurochem"},{"key":"2023101823044024300_JOCES_113_24_4511C7","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/S0092-8674(00)81649-9","article-title":"Essential Role of Phosphoinisitide Metabolism in Synaptic Vesicle Recycling","volume":"99","author":"Cremona","year":"1999","journal-title":"Cell"},{"key":"2023101823044024300_JOCES_113_24_4511C8","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1083\/jcb.127.4.915","article-title":"Induction of mutant dynamin specifically blocks endocytic coated vesicle formation","volume":"127","author":"Damke","year":"1994","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C9","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1074\/jbc.273.2.989","article-title":"Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein","volume":"273","author":"Dowbenko","year":"1998","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C10","first-page":"5814","article-title":"Eps15, a novel tyrosine kinase substrate, exhibits transforming activity","volume":"13","author":"Fazioli","year":"1993","journal-title":"Mol. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C11","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1006\/geno.1997.4645","article-title":"A novel SH3-containing human gene family preferentially expressed in the central nervous system","volume":"41","author":"Giachino","year":"1997","journal-title":"Genomics"},{"key":"2023101823044024300_JOCES_113_24_4511C12","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1126\/science.8430330","article-title":"Synaptic vesicle phosphoproteins and regulation of synaptic function","volume":"259","author":"Greengard","year":"1993","journal-title":"Science"},{"key":"2023101823044024300_JOCES_113_24_4511C13","doi-asserted-by":"publisher","first-page":"12990","DOI":"10.1074\/jbc.274.19.12990","article-title":"SAC1-like domains of yeast SAC1, INP52 and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases","volume":"274","author":"Guo","year":"1999","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C14","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1083\/jcb.133.4.761","article-title":"Association of a dynamin-like protein with the Golgi apparatus in mammalian cells","volume":"133","author":"Henley","year":"1996","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C15","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1038\/374190a0","article-title":"Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding","volume":"374","author":"Hinshaw","year":"1995","journal-title":"Nature"},{"key":"2023101823044024300_JOCES_113_24_4511C16","doi-asserted-by":"publisher","first-page":"31693","DOI":"10.1074\/jbc.274.44.31693","article-title":"Interaction of the Metalloprotease Disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, Endophilin I and SH3PX1","volume":"274","author":"Howard","year":"1999","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C17","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1083\/jcb.115.4.887","article-title":"An analysis of vertebrate mRNA sequences: Intimations of translational control","volume":"115","author":"Kozak","year":"1991","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C18","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/S0955-0674(98)80065-3","article-title":"Mechanisms of protein sorting and coat assembly: insights from the clathrin-coated vesicle pathway","volume":"10","author":"Le Borgne","year":"1998","journal-title":"Curr. Opin. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C19","doi-asserted-by":"publisher","first-page":"7320","DOI":"10.1093\/emboj\/17.24.7320","article-title":"A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion","volume":"17","author":"Li","year":"1998","journal-title":"EMBO J"},{"key":"2023101823044024300_JOCES_113_24_4511C20","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1126\/science.252.5009.1162","article-title":"Predicting coiled coils from protein sequences","volume":"252","author":"Lupas","year":"1991","journal-title":"Science"},{"key":"2023101823044024300_JOCES_113_24_4511C21","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/379353a0","article-title":"A presynaptic inositol-5-phosphatase","volume":"379","author":"McPherson","year":"1996","journal-title":"Nature"},{"key":"2023101823044024300_JOCES_113_24_4511C22","doi-asserted-by":"publisher","first-page":"23278","DOI":"10.1074\/jbc.272.37.23278","article-title":"FAP52, a novel, SH3 domain-containing focal adhesion protein","volume":"272","author":"Meril\u00e4inen","year":"1997","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C23","doi-asserted-by":"publisher","first-page":"5326","DOI":"10.1002\/j.1460-2075.1996.tb00917.x","article-title":"N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in PIP2-dependent manner downstream of tyrosine kinases","volume":"15","author":"Miki","year":"1996","journal-title":"EMBO J"},{"key":"2023101823044024300_JOCES_113_24_4511C24","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/34208","article-title":"Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP","volume":"391","author":"Miki","year":"1998","journal-title":"Nature"},{"key":"2023101823044024300_JOCES_113_24_4511C25","doi-asserted-by":"publisher","first-page":"6541","DOI":"10.1093\/emboj\/17.22.6541","article-title":"Recognition specificity of individual EH domains of mammals and yeast","volume":"17","author":"Paoluzi","year":"1998","journal-title":"EMBO J"},{"key":"2023101823044024300_JOCES_113_24_4511C26","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1046\/j.1432-1327.1998.2560201.x","article-title":"PACSIN, a brain protein that is upregulated upon differentiation into neuronal cells","volume":"256","author":"Plomann","year":"1998","journal-title":"Eur. J. Biochem"},{"key":"2023101823044024300_JOCES_113_24_4511C27","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1091\/mbc.10.2.501","article-title":"Syndapin I, a synaptic dynamin-binding protein that associates with the neural Wiskott-Aldrich syndrome protein","volume":"10","author":"Qualmann","year":"1999","journal-title":"Mol. Biol. Cell"},{"key":"2023101823044024300_JOCES_113_24_4511C28","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1083\/jcb.148.5.1047","article-title":"Syndapin isoforms participate in receptor-mediated endocytosis and actin organisation","volume":"148","author":"Qualmann","year":"2000","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C29","doi-asserted-by":"publisher","first-page":"8569","DOI":"10.1073\/pnas.94.16.8569","article-title":"The SH3p4\/SH3p8\/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain","volume":"94","author":"Ringstad","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023101823044024300_JOCES_113_24_4511C30","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/S0014-5793(99)00830-3","article-title":"PACSIN 2, a novel member of the PACSIN family of cytoplasmic adapter proteins","volume":"454","author":"Ritter","year":"1999","journal-title":"FEBS Lett"},{"key":"2023101823044024300_JOCES_113_24_4511C31","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/S0955-0674(98)80066-5","article-title":"Dynamin and its partners: a progress report","volume":"10","author":"Schmid","year":"1998","journal-title":"Curr. Opin. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C32","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/43613","article-title":"Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophophatic acid","volume":"401","author":"Schmidt","year":"1999","journal-title":"Nature"},{"key":"2023101823044024300_JOCES_113_24_4511C33","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1093\/emboj\/18.5.1159","article-title":"The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15","volume":"18","author":"Sengar","year":"1999","journal-title":"EMBO J"},{"key":"2023101823044024300_JOCES_113_24_4511C34","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1126\/science.276.5310.259","article-title":"Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions","volume":"276","author":"Shupliakov","year":"1997","journal-title":"Science"},{"key":"2023101823044024300_JOCES_113_24_4511C35","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1038\/10091","article-title":"SH3-domain-containing proteins function at distinct steps in clathrin-coated vesicle formation","volume":"1","author":"Simpson","year":"1999","journal-title":"Nature Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C36","doi-asserted-by":"publisher","first-page":"4547","DOI":"10.1016\/S0021-9258(17)41812-6","article-title":"Differential Expression and Regulation of Multiple Dynamins","volume":"269","author":"Sontag","year":"1994","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C37","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1083\/jcb.138.4.845","article-title":"PSTPIP: A tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for a PEST tyrosine phosphatase","volume":"138","author":"Spencer","year":"1997","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C38","doi-asserted-by":"publisher","first-page":"1474","DOI":"10.1126\/science.2506642","article-title":"Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins","volume":"245","author":"S\u00fcdhof","year":"1989","journal-title":"Science"},{"key":"2023101823044024300_JOCES_113_24_4511C39","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/9004","article-title":"Functional partnership between amphiphysin and dynamin in clathrin-medited endocytosis","volume":"1","author":"Takei","year":"1999","journal-title":"Nature Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C40","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.1083\/jcb.131.6.1789","article-title":"Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system","volume":"131","author":"Takei","year":"1995","journal-title":"J. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C41","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1073\/pnas.94.2.377","article-title":"The dynamins: Redundant or distinct functions for an expanding family of related GTPases?","volume":"94","author":"Urrutia","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023101823044024300_JOCES_113_24_4511C42","doi-asserted-by":"publisher","first-page":"5627","DOI":"10.1128\/MCB.15.10.5627","article-title":"Structure-function analysis of SH3 domains: SH3 binding specificity altered by single amino acid substitutions","volume":"15","author":"Weng","year":"1995","journal-title":"Mol. Cell Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C43","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/S0166-4328(00)00215-1","article-title":"Expression of nicotinic acetylcholine receptors in Alzheimer\u2019s disease: postmortem investigations and experimental approaches","volume":"113","author":"Wevers","year":"2000","journal-title":"Behav. Brain Res"},{"key":"2023101823044024300_JOCES_113_24_4511C44","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1016\/S0960-9822(06)00254-5","article-title":"Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain","volume":"7","author":"Wigge","year":"1997","journal-title":"Curr. Biol"},{"key":"2023101823044024300_JOCES_113_24_4511C45","doi-asserted-by":"publisher","first-page":"5765","DOI":"10.1074\/jbc.273.10.5765","article-title":"Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein","volume":"273","author":"Wu","year":"1998","journal-title":"J. Biol. Chem"},{"key":"2023101823044024300_JOCES_113_24_4511C46","doi-asserted-by":"publisher","first-page":"31401","DOI":"10.1074\/jbc.273.47.31401","article-title":"Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains","volume":"273","author":"Yamabhai","year":"1998","journal-title":"J. Biol. Chem"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/113\/24\/4511\/3237501\/joces_113_24_4511.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/113\/24\/4511\/3237501\/joces_113_24_4511.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T23:07:33Z","timestamp":1697670453000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/113\/24\/4511\/26376\/All-three-PACSIN-isoforms-bind-to-endocytic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12,15]]},"references-count":46,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2000,12,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.113.24.4511","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12,15]]}}}