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Multiple spontaneous transient increases in cytosolic free calcium [Ca2+]iwere observed in single human VSMCs migrating on type I collagen. Such [Ca2+]itransients were dependent on the presence of serum or PDGF-BB. Removal of serum, or loading cells with BAPTA, abolished the transients and decreased cell migration speed. The transients were not affected by disruption of cell polarization by dihydrocytochalasin B. Adhesion was used to investigate the specific role of cell-substrate interactions in the generation of transients. Transients are seen in VSMCs adhering either on collagen or on poly-L-lysine, suggesting that generation of transients is not strictly dependent on integrins. Buffering [Ca2+]iwith BAPTA led to accumulation of \u03b21 integrins at the cellular tail, and to increased release of integrin on the extracellular matrix. These results demonstrate a role for [Ca2+]itransients in the rapid, serum-dependent migration of VSMCs. These [Ca2+]itransients are present in migrating VSMCs only when two simultaneous events occur: (1) substrate independent spreading and (2) stimulation of cells by serum components such as PDGF-BB.<\/jats:p>","DOI":"10.1242\/jcs.113.4.653","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T03:22:01Z","timestamp":1619234521000},"page":"653-662","source":"Crossref","is-referenced-by-count":49,"title":["Migration of human vascular smooth muscle cells involves serum-dependent repeated cytosolic calcium transients"],"prefix":"10.1242","volume":"113","author":[{"given":"Arnaud","family":"Scherberich","sequence":"first","affiliation":[{"name":"Universit\u00e9 Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Mol\u00e9culaires, UMR CNRS 7034, Facult\u00e9 de Pharmacie , , Illkirch, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Campos-Toimil","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Mol\u00e9culaires, UMR CNRS 7034, Facult\u00e9 de Pharmacie , , Illkirch, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Rond\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Mol\u00e9culaires, UMR CNRS 7034, Facult\u00e9 de Pharmacie , , Illkirch, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Takeda","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Mol\u00e9culaires, UMR CNRS 7034, Facult\u00e9 de Pharmacie , , Illkirch, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Beretz","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Mol\u00e9culaires, UMR CNRS 7034, Facult\u00e9 de Pharmacie , , Illkirch, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2000,2,15]]},"reference":[{"key":"2024111914172794900_JOCES_113_4_653C1","first-page":"197","article-title":"Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins","volume":"50","author":"Aplin","year":"1998","journal-title":"Pharmacol. 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