{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:49:48Z","timestamp":1790009388719,"version":"4.0.1"},"reference-count":53,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2001,1,15]],"date-time":"2001-01-15T00:00:00Z","timestamp":979516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Distribution of mitochondria as well as other intracellular organelles in mammalian cells is regulated by interphase microtubules. Here, we demonstrate a role of microtubules in the mitochondrial biogenesis using various microtubule-active drugs and human osteosarcoma cell line 143B cells and rat liver-derived RL-34 cells. Depolymerization of microtubules by nocodazole or colchicine, as well as 2-methoxyestradiol, a natural estrogen metabolite, arrested asynchronously cultured cells in G2\/M phase of cell cycle and at the same time inhibited the mitochondrial mass increase and mtDNA replication. These drugs also inhibited the mitochondrial mass increase in the cells that were synchronized in cell cycle, which should occur during G1 to G2 phase progression in normal conditions. However, stabilization of microtubules by taxol did not affect the proliferation of mitochondria during the cell cycle, yet a prolonged incubation of cells with taxol induced an abnormal accumulation of mitochondria in cells arrested in G2\/M phase of cell cycle. Taxol-induced accumulation of mitochondria was not only demonstrated by mitochondria-specific fluorescent dyes but also evidenced by the examination of cells transfected with yellow fluorescent protein fused with mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (pEYFP) and by enhanced mtDNA replication. Two subpopulations of mitochondria were detected in taxol-treated cells: mitochondria with high \u0394\u03a8m, detectable either by Mito Tracker Red CMXRos or by Green FM, and those with low \u0394\u03a8m, detectable only by Green FM. However, taxol-induced increases in the mitochondrial mass and in the level of acetylated \u03b1-tubulin were abrogated by a co-treatment with taxol and nocodazole or taxol and colchicine. These data strongly suggest that interphase microtubules may be essential for the regulation of mitochondrial biogenesis in mammalian cells.<\/jats:p>","DOI":"10.1242\/jcs.114.2.281","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T18:41:11Z","timestamp":1619376071000},"page":"281-291","source":"Crossref","is-referenced-by-count":87,"title":["Opposite effects of microtubule-stabilizing and microtubule-destabilizing drugs on biogenesis of mitochondria in mammalian cells"],"prefix":"10.1242","volume":"114","author":[{"given":"Mariusz","family":"Karbowski","sequence":"first","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan H.","family":"Spodnik","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"},{"name":"Medical University of Gdansk 2 Department of Anatomy and Neurobiology , , Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masa-aki","family":"Teranishi","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Medical University of Gdansk 3 Department of General Chemistry , , Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Nishizawa","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Usukura","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"1 Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,1,15]]},"reference":[{"key":"2024011500320487300_JOCES_114_2_281C1","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1146\/annurev.cb.04.110188.001445","article-title":"Biogenesis of mitochondria","volume":"4","author":"Attardi","year":"1988","journal-title":"Annu. Rev. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C2","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1083\/jcb.111.2.495","article-title":"Individual microtubules in the axon consist of domains that differ in both composition and stability","volume":"111","author":"Baas","year":"1990","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0074-7696(08)61205-X","article-title":"Behavior of mitochondria in the living cell","volume":"122","author":"Bereiter-Hahn","year":"1990","journal-title":"Int. Rev. Cytol"},{"key":"2024011500320487300_JOCES_114_2_281C4","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0022-5320(69)90032-X","article-title":"Protein renewal in mitochondria as revealed by electron microscope radioautography","volume":"26","author":"Bergeron","year":"1969","journal-title":"J. Ultrastruct. Res"},{"key":"2024011500320487300_JOCES_114_2_281C5","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1006\/excr.1997.3899","article-title":"Distinct alterations in mitochondrial mass and function characterize different models of apoptosis","volume":"239","author":"Camilleri-Broet","year":"1998","journal-title":"Exp. Cell Res"},{"key":"2024011500320487300_JOCES_114_2_281C6","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1073\/pnas.81.3.788","article-title":"Lysosomes are associated with microtubules and not with intermediate filaments in cultured fibroblasts","volume":"81","author":"Collot","year":"1984","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024011500320487300_JOCES_114_2_281C7","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1083\/jcb.141.1.175","article-title":"Rho guanosine triphosphate mediates the selective stabilization of microtubules induced by lysophosphatidic acid","volume":"141","author":"Cook","year":"1998","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C8","doi-asserted-by":"publisher","first-page":"2041","DOI":"10.1021\/jm00012a003","article-title":"Synthesis, atitubulin and antomitotic activity, and cytotoxicity of analogs of 2-methoxyestradiol, an endogenous mammalian metabolite of estradiol that inhibitstubulin polymerization by binding to the cochicine binding site","volume":"38","author":"Cushman","year":"1995","journal-title":"J. Med. Chem"},{"key":"2024011500320487300_JOCES_114_2_281C9","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1083\/jcb.135.4.883","article-title":"In situ localization of mitochondrial DNA replication in intact mammalian cells","volume":"135","author":"Davis","year":"1996","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C10","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1177\/30.9.6182188","article-title":"A stable propidium iodide staining procedure for flow cytometry","volume":"30","author":"Deitch","year":"1982","journal-title":"J. Histochem. Cytochem"},{"key":"2024011500320487300_JOCES_114_2_281C11","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/0014-4827(76)90675-3","article-title":"Quantification of mitochondria during the cell cycle of Chinese hamster cells","volume":"99","author":"Devey","year":"1976","journal-title":"Exp. Cell Res"},{"key":"2024011500320487300_JOCES_114_2_281C12","doi-asserted-by":"publisher","first-page":"9673","DOI":"10.1074\/jbc.273.16.9673","article-title":"The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane proximal region","volume":"273","author":"De Vos","year":"1998","journal-title":"J. Biol. Chem"},{"key":"2024011500320487300_JOCES_114_2_281C13","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1083\/jcb.143.3.777","article-title":"Overexpression of Tau protein inhibits kinesin-dependent trafficking of vesicles. Mitochondria, and endoplasmic reticulum: implications for Alzheimer\u2019s disease","volume":"143","author":"Ebneth","year":"1998","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C14","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1006\/excr.1998.4057","article-title":"Toxin-induced activation of Rho GTP-binding protein increases Bcl-2 expression and influences mitochondrial homeostasis","volume":"242","author":"Fiorentini","year":"1998","journal-title":"Exp. Cell Res"},{"key":"2024011500320487300_JOCES_114_2_281C15","doi-asserted-by":"publisher","first-page":"1275","DOI":"10.1083\/jcb.131.5.1275","article-title":"Stable, detyrosinated microtubules function to localize vimentin intermediate filaments in fibroblasts","volume":"131","author":"Gurland","year":"1995","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C16","doi-asserted-by":"publisher","first-page":"2275","DOI":"10.1083\/jcb.109.5.2275","article-title":"Generation of stable, posttranslationally modified microtubule array is an early event in myogenic differentiation","volume":"109","author":"Gundersen","year":"1989","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C17","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1042\/bj1340935","article-title":"Biogenesis of mitochondria. A requirement for mitochondrial protein synthesis for the formation of anormal adenine nucleotide transporter un yeast","volume":"134","author":"Haslam","year":"1973","journal-title":"Biochem. J"},{"key":"2024011500320487300_JOCES_114_2_281C18","first-page":"266","volume-title":"In Handbook of Fluorescent Probes and Research Chemicalss","author":"Haugland","year":"1996","edition":"6th"},{"key":"2024011500320487300_JOCES_114_2_281C19","doi-asserted-by":"publisher","first-page":"3883","DOI":"10.1073\/pnas.74.9.3883","article-title":"Intracellular distributions of mechanochemical proteins in cultured fibroblasts","volume":"74","author":"Heggeness","year":"1977","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024011500320487300_JOCES_114_2_281C20","doi-asserted-by":"publisher","first-page":"3863","DOI":"10.1073\/pnas.75.8.3863","article-title":"Association of mitochondria with microtubules in cultured cells","volume":"75","author":"Heggeness","year":"1978","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024011500320487300_JOCES_114_2_281C21","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1126\/science.181.4101.749","article-title":"Mitochondria of yeasts: ultrastructural evidence for one giant, branched organelle per cell","volume":"181","author":"Hoffmann","year":"1973","journal-title":"Science"},{"key":"2024011500320487300_JOCES_114_2_281C22","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1083\/jcb.89.2.256","article-title":"Proliferation of mitochondria during the cell cycle of human cell line (HL-60)","volume":"89","author":"James","year":"1981","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C23","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/S0076-6879(98)98024-7","article-title":"Use of drugs to study role of microtubule assembly dynamics in living cells","volume":"298","author":"Jordan","year":"1998","journal-title":"Methods Enzymol"},{"key":"2024011500320487300_JOCES_114_2_281C24","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0167-4889(98)00167-0","article-title":"Cycloheximide and 4-OH-TEMPO suppress chloramphenicol-induced apoptosis in RL-34 cells via the suppression of the formation of megamitochondria","volume":"1449","author":"Karbowski","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"2024011500320487300_JOCES_114_2_281C25","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1042\/bj3480425","article-title":"Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells","volume":"348","author":"Lee","year":"2000","journal-title":"Biochem. J"},{"key":"2024011500320487300_JOCES_114_2_281C26","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/0014-4827(90)90219-Z","article-title":"Use of Nonyl Acridine orange and Rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle","volume":"186","author":"Leprat","year":"1990","journal-title":"Exp. Cell Res"},{"key":"2024011500320487300_JOCES_114_2_281C27","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/S0092-8674(00)81590-1","article-title":"Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis","volume":"94","author":"Li","year":"1998","journal-title":"Cell"},{"key":"2024011500320487300_JOCES_114_2_281C28","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.1073\/pnas.73.6.2082","article-title":"Biogenesis of mitochondria: molecular mapping of the mitochondrial genome of yeast","volume":"73","author":"Linnane","year":"1976","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024011500320487300_JOCES_114_2_281C29","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1073\/pnas.49.2.233","article-title":"Genesis of mitochondria in Neurospora crassa","volume":"49","author":"Luck","year":"1963","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024011500320487300_JOCES_114_2_281C30","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1083\/jcb.24.3.461","article-title":"Formation of mitochondria in Neurospora crassa","volume":"24","author":"Luck","year":"1965","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C31","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/S0014-5793(99)00344-0","article-title":"Quality control: from molecules to organelles","volume":"448","author":"Luzikov","year":"1999","journal-title":"FEBS Lett"},{"key":"2024011500320487300_JOCES_114_2_281C32","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/0006-291X(89)91700-2","article-title":"10-N Nonylacridine orange: a fluoresecent probe which stains mitochondria independently of their energetic state","volume":"164","author":"Maftah","year":"1989","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"2024011500320487300_JOCES_114_2_281C33","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1083\/jcb.138.2.449","article-title":"Mitochondrial proliferation and paradoxical membrane depolarization during terminal differentiation and apoptosis in a human colon carcinoma cell line","volume":"138","author":"Mancini","year":"1997","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C34","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.1016\/0092-8674(94)90012-4","article-title":"KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria","volume":"79","author":"Nangaku","year":"1994","journal-title":"Cell"},{"key":"2024011500320487300_JOCES_114_2_281C35","first-page":"29","article-title":"Membrane biogenesis: an overview","volume":"96","author":"Osafune","year":"1983","journal-title":"Methods Enzymol"},{"key":"2024011500320487300_JOCES_114_2_281C36","doi-asserted-by":"publisher","first-page":"3003","DOI":"10.1083\/jcb.111.6.3003","article-title":"Microtubules are stabilized in confluent epithelial cells but not in fibroblasts","volume":"111","author":"Pepperkok","year":"1990","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C37","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1083\/jcb.130.1.157","article-title":"Alterations in mitochondrial structure and function are early events of dexamethasone-induced apoptosis","volume":"130","author":"Petit","year":"1995","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C38","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1016\/0022-2836(71)90113-6","article-title":"Expression of the mitochondrial genome in HeLa cells. V. Transcription of mitochondrial DNA in relationship to the cell cycle","volume":"57","author":"Pica-Mattoccia","year":"1971","journal-title":"J. Mol. Biol"},{"key":"2024011500320487300_JOCES_114_2_281C39","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/0022-2836(72)90511-6","article-title":"Expression of the mitochondria genome in HeLa cells. IX. Replication of mitochondrial DNA in relationship to cell cycle in HeLa cells","volume":"64","author":"Pica-Mattoccia","year":"1972","journal-title":"J. Mol. Biol"},{"key":"2024011500320487300_JOCES_114_2_281C40","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1083\/jcb.104.2.289","article-title":"Microtubules containing acetylated \u03b1-tubulin in mammalian cells in culture","volume":"104","author":"Piperno","year":"1987","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C41","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1083\/jcb.74.2.468","article-title":"Mitochondrial growth and division during cell cycle in HeLa cells","volume":"74","author":"Posakony","year":"1977","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C42","doi-asserted-by":"publisher","first-page":"1763","DOI":"10.1126\/science.280.5370.1763","article-title":"Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses","volume":"280","author":"Rizzuto","year":"1998","journal-title":"Science"},{"key":"2024011500320487300_JOCES_114_2_281C43","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1083\/jcb.99.3.1092","article-title":"Association of elementss of the Golgiappratus with microtubules","volume":"99","author":"Rogalski","year":"1984","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C44","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1083\/jcb.139.4.963","article-title":"Micritubule stabilization in pressure overload cardiac hypertrophy","volume":"139","author":"Sato","year":"1997","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C45","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1083\/jcb.143.2.351","article-title":"A human dynamin-related protein controls the distribution of mitochondria","volume":"143","author":"Smirnowa","year":"1998","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C46","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1083\/jcb.56.3.833","article-title":"Mode of mitochondrial formation in HeLa cells","volume":"56","author":"Storrie","year":"1973","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C47","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1016\/S0092-8674(00)81459-2","article-title":"Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria","volume":"93","author":"Tanaka","year":"1998","journal-title":"Cell"},{"key":"2024011500320487300_JOCES_114_2_281C48","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1002\/cm.970290402","article-title":"Interaction among endoplasmic reticulum, microtubules, and retrograde movements of the cell surface","volume":"29","author":"Terasaki","year":"1994","journal-title":"Cell Motil. Cytoskelet"},{"key":"2024011500320487300_JOCES_114_2_281C49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0014-4827(85)80032-X","article-title":"Microtubules and the organization of the Golgi complex","volume":"159","author":"Thyberg","year":"1985","journal-title":"Exp. Cell Res"},{"key":"2024011500320487300_JOCES_114_2_281C50","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1083\/jcb.99.3.940","article-title":"Taxol-induced bundling of brain-derived microtubules","volume":"99","author":"Turner","year":"1984","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C51","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/S0092-8674(00)80450-X","article-title":"Bcl-xL regulates the membrane potential and volume of mitochondria","volume":"91","author":"Vander Heiden","year":"1977","journal-title":"Cell"},{"key":"2024011500320487300_JOCES_114_2_281C52","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1083\/jcb.136.1.71","article-title":"Visualization of the peroxisomal compartment in living mammalian cellss: dynamic behavior and association with microtubules","volume":"136","author":"Wiemer","year":"1997","journal-title":"J. Cell Biol"},{"key":"2024011500320487300_JOCES_114_2_281C53","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1126\/science.283.5407.1493","article-title":"The machinery of mitochondrial inheritance and behavior","volume":"283","author":"Yaffe","year":"1999","journal-title":"Science"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/114\/2\/281\/3354389\/joces_114_2_281.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/114\/2\/281\/3354389\/joces_114_2_281.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:32:44Z","timestamp":1705278764000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/114\/2\/281\/26542\/Opposite-effects-of-microtubule-stabilizing-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1,15]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.114.2.281","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1,15]]}}}