{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:21:23Z","timestamp":1790101283126,"version":"4.0.1"},"reference-count":50,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The regulation of endocytic traffic of receptors has central importance in the fine tuning of cell activities. Here, we provide evidence that cholesterol is required for the exit of cation-independent mannose 6-phosphate receptor (CI-MPR) from the endosomal carrier vesicle\/multivesicular bodies (ECV\/MVBs) to the Golgi. A previously established Chinese hamster ovary cell mutant, LEX2, exhibits arrested ECV\/MVBs in which CI-MPR and lysosomal glycoprotein-B (lgp-B) are accumulated. The abnormal accumulation of CI-MPR within the ECV\/MVBs in LEX2 cells was corrected in a post-translational manner by the supplementation of medium with cholesterol. Furthermore, it was shown that, by expression cloning using LEX2 mutant, the introduction of the NAD(P)H steroid dehydrogenase-like protein, an enzyme involved in the later stage of cholesterol biosynthesis, allows the exit of CI-MPR from the MVBs to the Golgi and reduces the number of arrested ECV\/MVBs in LEX2 cells. The recovery of the exit transport of CI-MPR from the ECV\/MVBs was associated with the restoration of the normal cellular free cholesterol level and segregation between CI-MPR and lgp-B, both of which had been localized at the internal small vesicles of the arrested ECV\/MVBs. By contrast, the restoration of cholesterol failed to correct the defective processing of endocytosed LDL to a degradative compartment in LEX2 cells. These results suggest that cholesterol is required for ECV\/MVB reorganization that drives the sorting\/transport of materials destined for the Golgi out of the pathways towards lysosomes.<\/jats:p>","DOI":"10.1242\/jcs.114.9.1765","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T00:24:46Z","timestamp":1619310286000},"page":"1765-1776","source":"Crossref","is-referenced-by-count":35,"title":["Cholesterol requirement for cation-independent mannose 6-phosphate receptor exit from multivesicular late endosomes to the Golgi"],"prefix":"10.1242","volume":"114","author":[{"given":"Ishido","family":"Miwako","sequence":"first","affiliation":[{"name":"1 Dept of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan"},{"name":"Kyoto University, Kyoto 606-8501 2 Dept of Biophysics, Faculty of Science , , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akitsugu","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Kansai Medical University, Osaka 570-8506 3 Dept of Physiology , , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kitamura","sequence":"additional","affiliation":[{"name":"University of Tokyo, Tokyo 108-8639 4 Dept of Hematopoietic Factors, The Institute of Medical Science , , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniaki","family":"Nagayama","sequence":"additional","affiliation":[{"name":"1 Dept of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Ohashi","sequence":"additional","affiliation":[{"name":"1 Dept of Molecular Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,5,1]]},"reference":[{"key":"2024011501004814500_JOCES_114_9_1765C1","doi-asserted-by":"publisher","first-page":"14624","DOI":"10.1074\/jbc.274.21.14624","article-title":"Cholesterol Biosynthesis from Lanosterol. 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