{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:23:03Z","timestamp":1791404583764,"version":"4.3.3"},"reference-count":41,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Huntington's disease (HD) is a dominantly inherited neurodegenerative disease caused by CAG expansion in the huntingtin gene, which adds a homopolymeric tract of polyglutamine (polyQ) to the encoded protein leading to the formation of toxic aggregates. Despite rapidly accumulating evidences supporting a role for intercellular transmission of protein aggregates, little is known about whether and how huntingtin (Htt) misfolding progresses through the brain. It has been recently reported that synthetic polyQ peptides and recombinant fragments of mutant Htt are readily internalized in cell cultures and able to seed polymerization of a reporter wild-type Htt. However there is no direct evidence of aggregate transfer between cells and the mechanism has not been explored. By expressing recombinant fragments of mutant Htt in neuronal cells and in primary neurons, here we show that aggregated fragments formed within one cell spontaneously transfer to neighbors in cell culture. We demonstrate that aggregates intercellular spreading requires cell-cell contact and does not occur upon aggregates secretion. Interestingly, we report that the expression of mutant, but not wild-type Htt fragments, increases the number of tunneling nanotubes (TNTs), which in turn provide an efficient mechanism of transfer.<\/jats:p>","DOI":"10.1242\/jcs.126086","type":"journal-article","created":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T02:30:31Z","timestamp":1371609031000},"source":"Crossref","is-referenced-by-count":146,"title":["Transfer of polyglutamine aggregates in neuronal cells occurs in tunneling nanotubes"],"prefix":"10.1242","author":[{"given":"Maddalena","family":"Costanzo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u00efda","family":"Abounit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovica","family":"Marzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Danckaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeina","family":"Chamoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Roux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Zurzolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2013,1,1]]},"reference":[{"key":"2021042613085932700_b1","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1242\/jcs.083279","article-title":"Wiring through tunneling nanotubes\u2014from electrical signals to organelle transfer.","volume":"125","author":"Abounit","year":"2012","journal-title":"J. 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