{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:42:21Z","timestamp":1782942141110,"version":"3.54.5"},"reference-count":50,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Rheb GTPase and the Tsc1-Tsc2 protein complex, which serves as a GTPase-activating protein for Rheb, play critical roles in the regulation of cell growth in response to extracellular conditions. In Schizosaccharomyces pombe, Rheb and Tsc1-Tsc2 regulate cell cycle progression, the onset of meiosis, and the uptake of amino acids. In cells lacking Tsc2 (\u0394tsc2), the amino acid transporter Aat1, which is normally expressed on the plasma membrane under starvation conditions, is confined to the Golgi. Here, we show that the loss of either pub1+, encoding an E3 ubiquitin ligase, or any1+, encoding a \u03b2-arrestin-like protein, allows constitutive expression of Aat1 on the plasma membrane in \u0394tsc2 cells, suggesting that Pub1 and Any1 are required for localization of Aat1 to the Golgi. Subsequent analysis revealed that in the Golgi, Pub1 and Any1 form a complex that ubiquitinates Aat1. Physical interaction of Pub1 and Any1 is more stable in \u0394tsc2 than in wild-type cells and is independent of Tor2 activity. These results indicate that the TSC-Rheb signaling pathway regulates localization of amino acid transporters via Pub1 and Any1 in Tor2-independent manner. Our study demonstrates that unlike budding yeast in which Rsp5 and ARTs, a pair of proteins analogous to Pub1 and Any1, respectively, primarily act to reduce expression of the transporters on PM when nutrients are abundant, the primary role of fission yeast Pub1 and Any1 is to store the transporter in the Golgi under nutrient-rich conditions.<\/jats:p>","DOI":"10.1242\/jcs.128355","type":"journal-article","created":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T22:23:03Z","timestamp":1375741383000},"source":"Crossref","is-referenced-by-count":23,"title":["Fission yeast Any1, \u03b2-arrestin-like protein, is involved in TSC-Rheb signaling and the regulation of amino acid transporters"],"prefix":"10.1242","author":[{"given":"Yukiko","family":"Nakase","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Nakase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Kashiwazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoka","family":"Murai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Otsubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Issei","family":"Mabuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Takegawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2013,1,1]]},"reference":[{"key":"2021042613072511900_b1","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1007\/s00438-008-0320-y","article-title":"The Tsc\/Rheb signaling pathway controls basic amino acid uptake via the Cat1 permease in fission yeast.","volume":"279","author":"Aspuria","year":"2008","journal-title":"Mol. 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