{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:22:04Z","timestamp":1759940524875},"reference-count":35,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1973,9,1]],"date-time":"1973-09-01T00:00:00Z","timestamp":115689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1973,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The fine-structural localization of peroxidases during ethylene-induced abscission of flower pedicels of Nicotiana tabacum L. cv. \u2018Little Turkish\u2019 has been investigated. Peroxidase activity has been localized in both the cell walls and intercellular spaces of ethylene-treated flower pedicels which were fixed in glutaraldehyde, incubated in diaminobenzidine (DAB) medium with postfixation in 2 % osmium tetroxide. Peroxidase staining is present in the cell walls and intercellular spaces of control tissue but is not as intense as in ethylene-treated tissue. Increased peroxidase staining is evident in the intercellular spaces and cell walls after 2 h of exposure to ethylene and increases in intensity between 2 and 5 h. At 5 h, ethylene-induced abscission occurs. Fine-structural investigations revealed prominent staining in the middle-lamellar and peripheral areas of the cell walls in ethylene-treated tissue. The peroxidase staining appears to be due to peroxidase as prior incubation with potassium cyanide gives a marked reduction in the staining reaction. Incubation of the ethylene-exposed tissue in aminotriazole, a specific inhibitor of catalase, does not reduce peroxidase staining, except in the microbodies, which reportedly contain catalase.<\/jats:p>","DOI":"10.1242\/jcs.13.2.591","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T04:47:39Z","timestamp":1619239659000},"page":"591-601","source":"Crossref","is-referenced-by-count":13,"title":["Peroxidases in tobacco abscission zone tissue i. fine-structural localization in cell walls during ethylene-induced abscission"],"prefix":"10.1242","volume":"13","author":[{"given":"E. W.","family":"Henry","sequence":"first","affiliation":[{"name":"Herbert H. Lehman College of the City University of Netv York Department of Biological Sciences , , Bronx, Neiv York 10468, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Herbert H. Lehman College of the City University of Netv York Department of Biological Sciences , , Bronx, Neiv York 10468, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1973,9,1]]},"reference":[{"key":"2023062014153133900_JOCES_13_2_591C1","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1111\/j.1469-185X.1970.tb01175.x","article-title":"Plant hormones in the control of abscission","volume":"45","author":"Addicott","year":"1970","journal-title":"Biol. Rev"},{"key":"2023062014153133900_JOCES_13_2_591C2","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1177\/17.1.62","article-title":"Demonstration of endogenous peroxidase activity in the electron microscope","volume":"17","author":"Behnke","year":"1969","journal-title":"J. Histochem. 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