{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:03:23Z","timestamp":1791119003565,"version":"4.1.0"},"reference-count":62,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Nemaline myopathy (NM) is a congenital myopathy with an estimated incidence of 1\u223650.000 live births, caused by mutations in thin filament components, including nebulin, accounting for about 50% of the cases. The identification of NM cases with nonsense mutations resulting in loss of the extreme C-terminal SH3 domain of nebulin suggests an important role of the nebulin SH3 domain, which is further supported by the recent demonstration of its role in IGF-1-induced sarcomeric actin filament formation through targeting of N-WASP to the Z-line. To provide further insights into the functional significance of the nebulin SH3 domain in the Z-disk and understand the mechanisms by which truncations of nebulin lead to NM, we took two approaches: 1) an affinity-based proteomic screening to identify novel interaction partners of the nebulin SH3 domain; and 2) generation and characterization of a novel knock-in mouse model with a premature stop codon in the nebulin gene, eliminating its C-terminal SH3 domain (Neb\u0394SH3 mouse). Surprisingly, detailed analyses of Neb\u0394SH3 mice revealed no structural or histological skeletal muscle abnormalities and no changes in gene expression or localization of interaction partners of the nebulin SH3 domain, including myopalladin, palladin, zyxin, and N-WASP. Also, no significant effect on peak isometric stress production, passive tensile stress, or Young's modulus was found. However, Neb\u0394SH3 muscle displayed a slightly altered force-frequency relationship and was significantly more susceptible to eccentric contraction-induced injury, suggesting that the nebulin SH3 domain plays a role in bearing high active stress and perhaps in fine-tuning the excitation-contraction coupling mechanism.<\/jats:p>","DOI":"10.1242\/jcs.137026","type":"journal-article","created":{"date-parts":[[2013,9,18]],"date-time":"2013-09-18T03:17:23Z","timestamp":1379474243000},"source":"Crossref","is-referenced-by-count":26,"title":["The nebulin SH3 domain is dispensable for normal skeletal muscle structure but is required for effective active load bearing in mouse"],"prefix":"10.1242","author":[{"given":"Daniel L.","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Vitiello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianlin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Gokhin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Castaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Coulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Piaser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carmela","family":"Filomena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Eggenhuizen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Kunderfranco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Camerini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Takano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Endo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Crescenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep","family":"Luther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Lieber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Louise","family":"Bang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2013,1,1]]},"reference":[{"key":"2021042613115347100_b1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1083\/jcb.153.2.413","article-title":"Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies.","volume":"153","author":"Bang","year":"2001","journal-title":"J. Cell Biol."},{"key":"2021042613115347100_b2","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1006\/jsbi.2002.4457","article-title":"Molecular dissection of the interaction of desmin with the C-terminal region of nebulin.","volume":"137","author":"Bang","year":"2002","journal-title":"J. Struct. Biol."},{"key":"2021042613115347100_b3","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1083\/jcb.200603119","article-title":"Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle.","volume":"173","author":"Bang","year":"2006","journal-title":"J. Cell Biol."},{"key":"2021042613115347100_b4","doi-asserted-by":"publisher","first-page":"4117","DOI":"10.1096\/fj.09-137729","article-title":"Nebulin plays a direct role in promoting strong actin-myosin interactions.","volume":"23","author":"Bang","year":"2009","journal-title":"FASEB J."},{"key":"2021042613115347100_b5","doi-asserted-by":"publisher","first-page":"C218","DOI":"10.1152\/ajpcell.00055.2007","article-title":"Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle.","volume":"293","author":"Barash","year":"2007","journal-title":"Am. J. Physiol."},{"key":"2021042613115347100_b6","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/S0166-4328(01)00297-2","article-title":"Controlling the false discovery rate in behavior genetics research.","volume":"125","author":"Benjamini","year":"2001","journal-title":"Behav. Brain Res."},{"key":"2021042613115347100_b7","doi-asserted-by":"publisher","first-page":"1856","DOI":"10.1016\/j.bpj.2008.10.053","article-title":"A nebulin ruler does not dictate thin filament lengths.","volume":"96","author":"Castillo","year":"2009","journal-title":"Biophys. J."},{"key":"2021042613115347100_b8","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1083\/jcb.116.6.1381","article-title":"An interaction between zyxin and alpha-actinin.","volume":"116","author":"Crawford","year":"1992","journal-title":"J. Cell Biol."},{"key":"2021042613115347100_b9","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1186\/1756-0500-5-513","article-title":"A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue.","volume":"5","author":"Dimauro","year":"2012","journal-title":"BMC Res. Notes"},{"key":"2021042613115347100_b10","doi-asserted-by":"publisher","first-page":"6222","DOI":"10.1074\/jbc.M707694200","article-title":"Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin.","volume":"283","author":"Dixon","year":"2008","journal-title":"J. Biol. Chem."},{"key":"2021042613115347100_b11","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/bioinformatics\/btn224","article-title":"lumi: a pipeline for processing Illumina microarray.","volume":"24","author":"Du","year":"2008","journal-title":"Bioinformatics"},{"key":"2021042613115347100_b12","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/1475-2859-4-34","article-title":"Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.","volume":"4","author":"D\u00fcmmler","year":"2005","journal-title":"Microb. Cell Fact."},{"key":"2021042613115347100_b13","doi-asserted-by":"publisher","first-page":"W451","DOI":"10.1093\/nar\/gkm296","article-title":"SH3-Hunter: discovery of SH3 domain interaction sites in proteins.","volume":"35 Web Server issue","author":"Ferraro","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2021042613115347100_b14","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1007\/s00109-005-0033-1","article-title":"The sarcomeric Z-disc: a nodal point in signalling and disease.","volume":"84","author":"Frank","year":"2006","journal-title":"J. Mol. Med."},{"key":"2021042613115347100_b15","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.ejcb.2008.01.010","article-title":"The role of palladin in actin organization and cell motility.","volume":"87","author":"Goicoechea","year":"2008","journal-title":"Eur. J. Cell Biol."},{"key":"2021042613115347100_b16","doi-asserted-by":"publisher","first-page":"e10347","DOI":"10.1371\/journal.pone.0010347","article-title":"Isoform-specific upregulation of palladin in human and murine pancreas tumors.","volume":"5","author":"Goicoechea","year":"2010","journal-title":"PLoS ONE"},{"key":"2021042613115347100_b17","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1038\/nrm3510","article-title":"A two-segment model for thin filament architecture in skeletal muscle.","volume":"14","author":"Gokhin","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042613115347100_b18","doi-asserted-by":"publisher","first-page":"C1123","DOI":"10.1152\/ajpcell.00503.2008","article-title":"Reduced thin filament length in nebulin-knockout skeletal muscle alters isometric contractile properties.","volume":"296","author":"Gokhin","year":"2009","journal-title":"Am. J. Physiol."},{"key":"2021042613115347100_b19","doi-asserted-by":"publisher","first-page":"11211","DOI":"10.1073\/pnas.1116065109","article-title":"The actin regulator N-WASp is required for muscle-cell fusion in mice.","volume":"109","author":"Gruenbaum-Cohen","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042613115347100_b20","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1002\/mus.10097","article-title":"Lack of the C-terminal domain of nebulin in a patient with nemaline myopathy.","volume":"25","author":"Gurgel-Giannetti","year":"2002","journal-title":"Muscle Nerve"},{"key":"2021042613115347100_b21","doi-asserted-by":"publisher","first-page":"D412","DOI":"10.1093\/nar\/gkn760","article-title":"STRING 8\u2014a global view on proteins and their functional interactions in 630 organisms.","volume":"37 Database issue","author":"Jensen","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"2021042613115347100_b22","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1074\/mcp.M500108-MCP200","article-title":"Novel Src homology 3 domain-binding motifs identified from proteomic screen of a Pro-rich region.","volume":"4","author":"Jia","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"2021042613115347100_b23","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1016\/S0092-8674(02)01226-6","article-title":"The cardiac mechanical stretch sensor machinery involves a Z disc complex that is defective in a subset of human dilated cardiomyopathy.","volume":"111","author":"Kn\u00f6ll","year":"2002","journal-title":"Cell"},{"key":"2021042613115347100_b24","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1152\/physrev.00017.2009","article-title":"Muscle giants: molecular scaffolds in sarcomerogenesis.","volume":"89","author":"Kontrogianni-Konstantopoulos","year":"2009","journal-title":"Physiol. Rev."},{"key":"2021042613115347100_b25","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1016\/j.jmb.2006.07.077","article-title":"Expression of distinct classes of titin isoforms in striated and smooth muscles by alternative splicing, and their conserved interaction with filamins.","volume":"362","author":"Labeit","year":"2006","journal-title":"J. Mol. Biol."},{"key":"2021042613115347100_b26","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1021\/pr3000199","article-title":"Interaction network of the 14-3-3 protein in the ancient protozoan parasite Giardia duodenalis.","volume":"11","author":"Lalle","year":"2012","journal-title":"J. Proteome Res."},{"key":"2021042613115347100_b27","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1002\/humu.20370","article-title":"Identification of 45 novel mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.","volume":"27","author":"Lehtokari","year":"2006","journal-title":"Hum. Mutat."},{"key":"2021042613115347100_b28","doi-asserted-by":"publisher","first-page":"20401","DOI":"10.1074\/jbc.M310304200","article-title":"Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1.","volume":"279","author":"Li","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2021042613115347100_b29","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/j.semcdb.2008.08.009","article-title":"Thin filament length regulation in striated muscle sarcomeres: pointed-end dynamics go beyond a nebulin ruler.","volume":"19","author":"Littlefield","year":"2008","journal-title":"Semin. Cell Dev. Biol."},{"key":"2021042613115347100_b30","doi-asserted-by":"publisher","first-page":"27484","DOI":"10.1074\/jbc.272.43.27484","article-title":"Comparison of three members of the cysteine-rich protein family reveals functional conservation and divergent patterns of gene expression.","volume":"272","author":"Louis","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2021042613115347100_b31","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/s10974-009-9189-6","article-title":"The vertebrate muscle Z-disc: sarcomere anchor for structure and signalling.","volume":"30","author":"Luther","year":"2009","journal-title":"J. Muscle Res. Cell Motil."},{"key":"2021042613115347100_b32","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/S0014-5793(02)03655-4","article-title":"Interaction of nebulin SH3 domain with titin PEVK and myopalladin: implications for the signaling and assembly role of titin and nebulin.","volume":"532","author":"Ma","year":"2002","journal-title":"FEBS Lett."},{"key":"2021042613115347100_b33","doi-asserted-by":"publisher","first-page":"27539","DOI":"10.1074\/jbc.M604525200","article-title":"Titin as a giant scaffold for integrating stress and Src homology domain 3-mediated signaling pathways: the clustering of novel overlap ligand motifs in the elastic PEVK segment.","volume":"281","author":"Ma","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2021042613115347100_b34","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1242\/jcs.114.7.1253","article-title":"SH3 domains: complexity in moderation.","volume":"114","author":"Mayer","year":"2001","journal-title":"J. Cell Sci."},{"key":"2021042613115347100_b35","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1006\/jmbi.1998.1999","article-title":"Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs.","volume":"282","author":"Millevoi","year":"1998","journal-title":"J. Mol. Biol."},{"key":"2021042613115347100_b36","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1111\/j.1469-8749.2007.00907.x","article-title":"Increased efficacy and decreased systemic-effects of botulinum toxin A injection after active or passive muscle manipulation.","volume":"49","author":"Minamoto","year":"2007","journal-title":"Dev. Med. Child Neurol."},{"key":"2021042613115347100_b37","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0014-5793(92)80901-R","article-title":"SH3 \u2013 an abundant protein domain in search of a function.","volume":"307","author":"Musacchio","year":"1992","journal-title":"FEBS Lett."},{"key":"2021042613115347100_b38","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1136\/jmg.34.9.705","article-title":"Nemaline myopathy: current concepts.","volume":"34","author":"North","year":"1997","journal-title":"J. Med. Genet."},{"key":"2021042613115347100_b39","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1096\/fj.10-176727","article-title":"Disrupted myosin cross-bridge cycling kinetics triggers muscle weakness in nebulin-related myopathy.","volume":"25","author":"Ochala","year":"2011","journal-title":"FASEB J."},{"key":"2021042613115347100_b40","doi-asserted-by":"publisher","first-page":"2912","DOI":"10.1096\/fj.07-104372","article-title":"Sarcoplasmic reticulum calcium uptake and speed of relaxation are depressed in nebulin-free skeletal muscle.","volume":"22","author":"Ottenheijm","year":"2008","journal-title":"FASEB J."},{"key":"2021042613115347100_b41","doi-asserted-by":"publisher","first-page":"2359","DOI":"10.1093\/hmg\/ddp168","article-title":"Thin filament length dysregulation contributes to muscle weakness in nemaline myopathy patients with nebulin deficiency.","volume":"18","author":"Ottenheijm","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"2021042613115347100_b42","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.jsb.2009.11.013","article-title":"Altered myofilament function depresses force generation in patients with nebulin-based nemaline myopathy (NEM2).","volume":"170","author":"Ottenheijm","year":"2010","journal-title":"J. Struct. Biol."},{"key":"2021042613115347100_b43","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1093\/hmg\/ddr084","article-title":"Changes in cross-bridge cycling underlie muscle weakness in patients with tropomyosin 3-based myopathy.","volume":"20","author":"Ottenheijm","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"2021042613115347100_b44","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1091\/mbc.E07-07-0690","article-title":"Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.","volume":"19","author":"Pappas","year":"2008","journal-title":"Mol. Biol. Cell"},{"key":"2021042613115347100_b45","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1083\/jcb.201001043","article-title":"Nebulin regulates actin filament lengths by a stabilization mechanism.","volume":"189","author":"Pappas","year":"2010","journal-title":"J. Cell Biol."},{"key":"2021042613115347100_b46","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1073\/pnas.96.5.2305","article-title":"Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.","volume":"96","author":"Pelin","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042613115347100_b47","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1006\/jmbi.1997.1521","article-title":"SH3 in muscles: solution structure of the SH3 domain from nebulin.","volume":"276","author":"Politou","year":"1998","journal-title":"J. Mol. Biol."},{"key":"2021042613115347100_b48","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1006\/jmbi.2001.5312","article-title":"The SH3 domain of nebulin binds selectively to type II peptides: theoretical prediction and experimental validation.","volume":"316","author":"Politou","year":"2002","journal-title":"J. Mol. Biol."},{"key":"2021042613115347100_b49","doi-asserted-by":"publisher","first-page":"7956","DOI":"10.1073\/pnas.92.17.7956","article-title":"Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).","volume":"92","author":"Reinhard","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042613115347100_b50","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/S0092-8674(00)80732-1","article-title":"The interaction between N-WASP and the Arp2\/3 complex links Cdc42-dependent signals to actin assembly.","volume":"97","author":"Rohatgi","year":"1999","journal-title":"Cell"},{"key":"2021042613115347100_b51","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.febslet.2004.04.006","article-title":"Molecular analysis of the interaction between palladin and alpha-actinin.","volume":"566","author":"R\u00f6nty","year":"2004","journal-title":"FEBS Lett."},{"key":"2021042613115347100_b52","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.tcm.2007.09.002","article-title":"\u201cZ\u201deroing in on the role of Cypher in striated muscle function, signaling, and human disease.","volume":"17","author":"Sheikh","year":"2007","journal-title":"Trends Cardiovasc. Med."},{"key":"2021042613115347100_b53","doi-asserted-by":"publisher","first-page":"D561","DOI":"10.1093\/nar\/gkq973","article-title":"The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored.","volume":"39 Database issue","author":"Szklarczyk","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"2021042613115347100_b54","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1126\/science.1197767","article-title":"Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation.","volume":"330","author":"Takano","year":"2010","journal-title":"Science"},{"key":"2021042613115347100_b55","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1242\/jcs.042234","article-title":"Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle.","volume":"123","author":"Tonino","year":"2010","journal-title":"J. Cell Sci."},{"key":"2021042613115347100_b56","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s10974-012-9286-9","article-title":"Axial distribution of myosin binding protein-C is unaffected by mutations in human cardiac and skeletal muscle.","volume":"33","author":"Vydyanath","year":"2012","journal-title":"J. Muscle Res. Cell Motil."},{"key":"2021042613115347100_b57","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1016\/S0960-8966(02)00065-2","article-title":"Mutations in the nebulin gene can cause severe congenital nemaline myopathy.","volume":"12","author":"Wallgren-Pettersson","year":"2002","journal-title":"Neuromuscul. Disord."},{"key":"2021042613115347100_b58","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.spen.2011.10.004","article-title":"Nemaline myopathies.","volume":"18","author":"Wallgren-Pettersson","year":"2011","journal-title":"Semin. Pediatr. Neurol."},{"key":"2021042613115347100_b59","doi-asserted-by":"publisher","first-page":"2199","DOI":"10.1083\/jcb.107.6.2199","article-title":"Architecture of the sarcomere matrix of skeletal muscle: immunoelectron microscopic evidence that suggests a set of parallel inextensible nebulin filaments anchored at the Z line.","volume":"107","author":"Wang","year":"1988","journal-title":"J. Cell Biol."},{"key":"2021042613115347100_b60","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1152\/japplphysiol.01242.2001","article-title":"Functional characteristics of dystrophic skeletal muscle: insights from animal models.","volume":"93","author":"Watchko","year":"2002","journal-title":"J. Appl. Physiol."},{"key":"2021042613115347100_b61","doi-asserted-by":"publisher","first-page":"3843","DOI":"10.1038\/sj.emboj.7601242","article-title":"Nebulin regulates thin filament length, contractility, and Z-disk structure in vivo.","volume":"25","author":"Witt","year":"2006","journal-title":"EMBO J."},{"key":"2021042613115347100_b62","doi-asserted-by":"publisher","first-page":"C1175","DOI":"10.1152\/ajpcell.00049.2008","article-title":"Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture.","volume":"294","author":"Zhang","year":"2008","journal-title":"Am. J. Physiol."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/126\/23\/5477\/1933861\/jcs-126-23-5477.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.137026\/2045851\/jcs137026.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.137026\/2045851\/jcs137026.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T18:22:56Z","timestamp":1621362176000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/doi\/10.1242\/jcs.137026\/263813\/The-nebulin-SH3-domain-is-dispensable-for-normal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,1]]},"references-count":62,"URL":"https:\/\/doi.org\/10.1242\/jcs.137026","relation":{},"ISSN":["1477-9137","0021-9533"],"issn-type":[{"value":"1477-9137","type":"electronic"},{"value":"0021-9533","type":"print"}],"subject":[],"published":{"date-parts":[[2013,1,1]]},"article-number":"jcs.137026"}}