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Knockdown of sept7b by morpholino antisense oligonucleotides caused misorientation of basal bodies and cilia, reduction of apical actin, and shortening of motile cilia in Kupffer's vesicle and pronephric tubules. This resulted in pericardial and yolk sac edema, body axis curvature, and hydrocephaly. Significantly, in sept7b morphants we detected strong left-right asymmetry defects in the heart and lateral plate mesoderm (situs inversus), reduced fluid flow in the kidney, the formation of kidney cysts, and loss of glomerular filtration barrier function. Thus, sept7b is essential in zebrafish development for pronephric function and ciliogenesis, and loss of sept7b expression results in defects that resemble human ciliopathies.<\/jats:p>","DOI":"10.1242\/jcs.138495","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T04:19:16Z","timestamp":1391573956000},"source":"Crossref","is-referenced-by-count":21,"title":["<i>Sept7b<\/i>is essential for pronephric function and development of left-right asymmetry in zebrafish embryogenesis"],"prefix":"10.1242","author":[{"given":"Surjya Narayan","family":"Dash","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eero","family":"Lehtonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita A.","family":"Wasik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"Schepis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jere","family":"Paavola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pertti","family":"Panula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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