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Cdc42 activation is mediated by the RhoGEF, intersectin-1 (ITSN1), which is recruited to the virus prior to actin-based motility. Moreover, Cdc42-ITSN1-N-WASP function collaboratively in a feed-forward loop to promote vaccinia-induced actin polymerization. Outside the context of infection, we demonstrate that ITSN1 also functions together with Cdc42, Nck and N-WASP during Fc gamma receptor-mediated phagocytosis. Our observations suggest that ITSN1 is an important general regulator of Cdc42, Nck and N-WASP-dependent actin polymerization.<\/jats:p>","DOI":"10.1242\/jcs.141366","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T22:51:26Z","timestamp":1385592686000},"source":"Crossref","is-referenced-by-count":53,"title":["Cdc42 and the RhoGEF Intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation"],"prefix":"10.1242","author":[{"given":"Ashley C.","family":"Humphries","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara K.","family":"Donnelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Way","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2013,1,1]]},"reference":[{"key":"2021042614234606800_b1","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1038\/20726","article-title":"Structure of Cdc42 in complex with the GTPase-binding domain of the \u2018Wiskott-Aldrich syndrome\u2019 protein.","volume":"399","author":"Abdul-Manan","year":"1999","journal-title":"Nature"},{"key":"2021042614234606800_b2","doi-asserted-by":"publisher","first-page":"20992","DOI":"10.1074\/jbc.273.33.20992","article-title":"The Wiskott-Aldrich syndrome protein-interacting protein (WIP) binds to the adaptor protein Nck.","volume":"273","author":"Ant\u00f3n","year":"1998","journal-title":"J. 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