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Such a role required lysosomal acidification and exocytosis. Specifically, we found that the inhibition of lysosomal acidification using Bafilomycin A1 (Baf) lead to a redistribution of Zn2+ pools, and increased apoptosis. Additionally, the inhibition of lysosomal exocytosis through knockdown (KD) of the lysosomal SNARE proteins VAMP7 and Synaptotagmin VII (SYT7) suppressed Zn2+ secretion and VAMP7 KD cells had increased apoptosis. These data show that lysosomes play a central role in Zn2+ handling, suggesting a novel Zn2+ detoxification pathway.<\/jats:p>","DOI":"10.1242\/jcs.145318","type":"journal-article","created":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T07:48:48Z","timestamp":1400140128000},"source":"Crossref","is-referenced-by-count":44,"title":["Zinc efflux through lysosomal exocytosis prevents zinc-induced toxicity"],"prefix":"10.1242","author":[{"given":"Ira","family":"Kukic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon L.","family":"Kelleher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill","family":"Kiselyov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2021042613302966500_b1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1023\/A:1012932712483","article-title":"Cellular zinc sensors: MTF-1 regulation of gene expression.","volume":"14","author":"Andrews","year":"2001","journal-title":"Biometals"},{"key":"2021042613302966500_b2","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1189\/jlb.1208759","article-title":"Zinc transporter ZIP8 (SLC39A8) and zinc influence IFN-gamma expression in activated human T cells.","volume":"86","author":"Aydemir","year":"2009","journal-title":"J. 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