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Knockdown of fibronectin with siRNAs also blocked condensation. Fibrillar fibronectin matrix is detected prior to cell condensation and levels increased during and after condensation. Inhibition of fibronectin matrix assembly by the functional upstream domain (FUD) prevented cell condensation by MSCs and also by the chondrogenic cell line ATDC5. Our data show that cell condensation and induction of chondrogenesis depend on fibronectin matrix assembly and DTDST and indicate that this transporter is required earlier in chondrogenesis than previously appreciated. They also raise the possibility that certain of the skeletal defects in DTD patients might derive from the link between DTDST, fibronectin matrix, and condensation.<\/jats:p>","DOI":"10.1242\/jcs.150276","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T05:18:56Z","timestamp":1408684736000},"source":"Crossref","is-referenced-by-count":35,"title":["Fibronectin matrix assembly is essential for cell condensation during chondrogenesis"],"prefix":"10.1242","author":[{"given":"Purva","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean E.","family":"Schwarzbauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2021042613420010700_b1","doi-asserted-by":"crossref","first-page":"27863","DOI":"10.1016\/S0021-9258(18)46866-4","article-title":"Fibronectin self-association is mediated by complementary sites within the amino-terminal one-third of the molecule.","volume":"269","author":"Aguirre","year":"1994","journal-title":"J. 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