{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:10:59Z","timestamp":1761919859423,"version":"3.41.2"},"reference-count":41,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Accurate cell division requires proper assembly of high-order septin structures. In fission yeast, Spn1-4 are assembled into a primary septin ring at the division site, and the subsequent recruitment of Mid2 to the structure results in a stable septin ring. However, not much is known about the regulation of this key process. Here, we found deletion of Spt20, a structural subunit of SAGA transcriptional activation complex, caused a severe cell separation defect. The defect is mainly due to impaired septin ring assembly, as 80% of spt20\u0394 cells lost septin rings at the division sites. Spt20 regulates septin ring assembly partially through the transcriptional activation of mid2+. Spt20 also interacts with Spn2 and Mid2 in vitro and is associated with other components of the ring in vivo. Spt20 is co-localized with the septin ring, but does not separate when the septin ring splits. Importantly, Spt20 regulates the stability of the septin ring and is required for the recruitment of Mid2. The transcription-dependent and -independent roles of Spt20 in the septin ring assembly highlight a multifaceted regulation of one process by a SAGA subunit.<\/jats:p>","DOI":"10.1242\/jcs.151910","type":"journal-article","created":{"date-parts":[[2014,7,12]],"date-time":"2014-07-12T04:52:31Z","timestamp":1405140751000},"source":"Crossref","is-referenced-by-count":2,"title":["Septin ring assembly is regulated by Spt20, a structural subunit of SAGA complex"],"prefix":"10.1242","author":[{"given":"Bingkun","family":"Lei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchen","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Wen","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2021042613315781800_b1","doi-asserted-by":"publisher","first-page":"2003","DOI":"10.1091\/mbc.E04-06-0442","article-title":"Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe.","volume":"16","author":"Alonso-Nu\u00f1ez","year":"2005","journal-title":"Mol. 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