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By imaging the spatiotemporal dynamics of fluorescent markers for PIP3, PTEN, and filamentous actin, we observed that the activity of the signaling pathway remained spatially confined to the stimulated membrane region. Neighboring parts of the membrane were not excited and no receptor-initiated spatial spreading of excitation waves was observed. To generate localized cAMP stimuli, either particles that carried covalently bound cAMP molecules on their surface were brought into contact with the cell, or a patch of the cell membrane was aspirated into a glass micropipette to shield this patch against freely diffusing cAMP molecules in the surrounding medium. Additionally, also the binding site of the cAMP receptor was probed with different surface-immobilized cAMP molecules, confirming results from earlier ligand binding studies.<\/jats:p>","DOI":"10.1242\/jcs.161133","type":"journal-article","created":{"date-parts":[[2014,10,10]],"date-time":"2014-10-10T03:00:12Z","timestamp":1412910012000},"source":"Crossref","is-referenced-by-count":6,"title":["Signaling in chemotactic amoebae remains spatially confined to stimulated membrane regions"],"prefix":"10.1242","author":[{"given":"Matthias","family":"Gerhardt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Walz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Beta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2021042613410344900_b1","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1007\/s11010-009-0111-8","article-title":"Dictyostelium discoideum \u2013 a model for many reasons.","volume":"329","author":"Annesley","year":"2009","journal-title":"Mol. 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