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Concordantly, FAM65B-deficiency in neutrophils resulted in an increase in RHOA activity and front localization of pMLC as well as defects in chemotaxis directionality and adhesion to endothelial cells under flow. These data together elucidate a mechanism for RHOA and pMLC polarization in stimulated neutrophils through direct inhibition of RHOA by FAM65B at the leading edge.<\/jats:p>","DOI":"10.1242\/jcs.161497","type":"journal-article","created":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T05:51:59Z","timestamp":1421301119000},"source":"Crossref","is-referenced-by-count":16,"title":["Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils"],"prefix":"10.1242","author":[{"given":"Kun","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingzhao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dejie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenji","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianqing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2015,1,1]]},"reference":[{"key":"2021042613495055000_b1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/419021a","article-title":"A chemical compass.","volume":"419","author":"Bourne","year":"2002","journal-title":"Nature"},{"key":"2021042613495055000_b2","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0378-1119(96)00587-2","article-title":"PL48: a novel gene associated with cytotrophoblast and lineage-specific HL-60 cell differentiation.","volume":"185","author":"Dakour","year":"1997","journal-title":"Gene"},{"key":"2021042613495055000_b3","doi-asserted-by":"publisher","first-page":"9864","DOI":"10.1073\/pnas.1401950111","article-title":"FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.","volume":"111","author":"Diaz-Horta","year":"2014","journal-title":"Proc. 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