{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:53:05Z","timestamp":1790070785133,"version":"4.0.1"},"reference-count":38,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Nuclear functions including gene expression, DNA replication, and genome maintenance intimately rely on dynamic changes in chromatin organization. The movements of chromatin fibers may play important roles in the regulation of these fundamental processes, yet the mechanisms controlling chromatin mobility are poorly understood due to methodological limitations for the assessment of chromatin movements. Here, we present a facile and quantitative technique that relies on photoactivation of GFP-tagged histones and paired-particle tracking to measure chromatin mobility in live cells. We validate the method by comparing live cells to ATP-depleted cells and show that chromatin movements in mammalian cells are predominantly energy-dependent. We also find that chromatin diffusion decreases in response to DNA breaks induced by a genotoxic drug or by the ISceI meganuclease. Time course analysis after cell exposure to ionizing radiations indicates that the decrease in chromatin mobility is transient and precedes subsequent increased mobility. Future applications of the method in the DNA repair field and beyond are discussed.<\/jats:p>","DOI":"10.1242\/jcs.161885","type":"journal-article","created":{"date-parts":[[2014,12,13]],"date-time":"2014-12-13T02:51:30Z","timestamp":1418439090000},"source":"Crossref","is-referenced-by-count":14,"title":["Nanoscale histone localization in live cells reveals reduced chromatin mobility in response to DNA damage"],"prefix":"10.1242","author":[{"given":"Jing","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Alexandre","family":"Vidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie A.","family":"Leli\u00e8vre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M. 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