{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:14:05Z","timestamp":1790219645877,"version":"4.1.0"},"reference-count":42,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The Rho GTPase Cdc42 regulates key signaling pathways required for multiple cell functions, including maintenance of shape, polarity, proliferation, migration, differentiation, and morphogenesis. Although previous studies have shown that Cdc42 is required for proper epithelial development and maintenance, its exact molecular function in kidney development is not well understood. In this study, we define the specific role of Cdc42 during murine kidney epithelial tubulogenesis by deleting it selectively at the initiation of ureteric bud or metanephric mesenchyme development. Deletion in either lineage results in abnormal tubulogenesis, with profound defects in polarity, lumen formation, and the actin cytoskeleton. Ultimately, these defects lead to renal failure. Additionally, in vitro analysis of Cdc42-null collecting duct cells shows that Cdc42 controls these processes by regulating the polarity Par complex (Par3\/Par6\/aPKC\/Cdc42) and the cytoskeletal proteins N-Wasp and ezrin. Thus, we conclude that the principal role of Cdc42 in ureteric bud and metanephric mesenchyme development is to regulate epithelial cell polarity and the actin cytoskeleton.<\/jats:p>","DOI":"10.1242\/jcs.164509","type":"journal-article","created":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T02:00:45Z","timestamp":1445479245000},"source":"Crossref","is-referenced-by-count":30,"title":["Cdc42 regulates epithelial cell polarity and cytoskeletal function in kidney tubule development"],"prefix":"10.1242","author":[{"given":"Bertha C.","family":"Elias","sequence":"first","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrita","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Texas Southwestern Medical Center, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diptiben V.","family":"Parekh","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda","family":"Mernaugh","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Adams","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhufeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Texas Southwestern Medical Center, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cord","family":"Brakebusch","sequence":"additional","affiliation":[{"name":"Biotech Research Center, University of Copenhagen, Ole Maal\u00f8es Vej 5, DK-2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambra","family":"Pozzi","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"},{"name":"Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"},{"name":"Veterans Affairs Hospital, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise K.","family":"Marciano","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Texas Southwestern Medical Center, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Texas Southwestern Medical Center, Texas, 75390, USA"},{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Texas, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy","family":"Zent","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"},{"name":"Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"},{"name":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA"},{"name":"Veterans Affairs Hospital, Nashville, Tennessee 37232, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2015,1,1]]},"reference":[{"key":"2021042614473487000_JCS164509C1","doi-asserted-by":"publisher","first-page":"3037","DOI":"10.1242\/jcs.052704","article-title":"Actin machinery and mechanosensitivity in invadopodia, podosomes and focal adhesions","volume":"122","author":"Albiges-Rizo","year":"2009","journal-title":"J. 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