{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:01:42Z","timestamp":1771002102731,"version":"3.50.1"},"reference-count":40,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1975,5,1]],"date-time":"1975-05-01T00:00:00Z","timestamp":168134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1975,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Hybridization of mutant cell lines deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT; E.C.: 2.4.2.8) from a variety of established rodent sources with HGPRT+ human cells yielded progeny cells which grew in selective medium containing hypoxanthine, aminopterin and thymidine (HAT). The same result was obtained when the human cell used was an HGPRT- transformed line derived from a patient with the Lesch-Nyhan syndrome. Electrophoretic analysis indicated that all HAT-resistant progeny clones contained an active HGPRT enzyme which was indistinguishable from the wild type enzyme of the corresponding normal rodent cells. In contrast, no HAT-resistant cells have been obtained when the same HGPRT- rodent cells were subjected to fusion processes in the absence of human cells or when they were fused with similarly derived HGPRT- mutant cells of other rodents. Reversion in expression of the rodent gene for HGPRT was detected in clones which retained one or more human chromosomes and in clones which contained no detectable human chromosomal material. The observed re-expression of rodent HGPRT in HAT-resistant clones suggests that HGPRT+ as well as HGPRT- human cells contributed a factor which determined the expression of respective rodent structural genes for HGPRT. In contrast, HGPRT- rodent cells were unable to induce the synthesis of normal HGPRT in the cells derived from the patient with the Lesch-Nyhan syndrome.<\/jats:p>","DOI":"10.1242\/jcs.17.3.567","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T20:37:12Z","timestamp":1619469432000},"page":"567-578","source":"Crossref","is-referenced-by-count":18,"title":["Reversion in expression of hypoxanthine-guanine phosphoribosyl transferase following cell hybridization"],"prefix":"10.1242","volume":"17","author":[{"given":"B.","family":"Bakay","sequence":"first","affiliation":[{"name":"University of California-San Diego Department of Pediatrics , , La Jolla, California, U.S.A. and the Wistar Institute, Philadelphia, Pennsylvania, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. L.","family":"Nyhan","sequence":"additional","affiliation":[{"name":"University of California-San Diego Department of Pediatrics , , La Jolla, California, U.S.A. and the Wistar Institute, Philadelphia, Pennsylvania, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Croce","sequence":"additional","affiliation":[{"name":"University of California-San Diego Department of Pediatrics , , La Jolla, California, U.S.A. and the Wistar Institute, Philadelphia, Pennsylvania, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Koprowski","sequence":"additional","affiliation":[{"name":"University of California-San Diego Department of Pediatrics , , La Jolla, California, U.S.A. and the Wistar Institute, Philadelphia, Pennsylvania, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1975,5,1]]},"reference":[{"key":"2023053020245725100_JOCES_17_3_567C1","doi-asserted-by":"publisher","first-page":"1805","DOI":"10.1172\/JCI106982","article-title":"Hypoxanthine-guanine phosphoribosyltransferase: characteristics of the mutant enzyme in erythrocytes from patients with the Lesch-Nyhan syndrome","volume":"g1","author":"Arnold","year":"1972","journal-title":"J. clin. Invest"},{"key":"2023053020245725100_JOCES_17_3_567C2","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/0003-2697(71)90403-9","article-title":"Detection of radioactive components in polyacrylamide gel disc electropherograms by automated mechanical fractionation","volume":"40","author":"Bakay","year":"1971","journal-title":"Analyt. Biochem"},{"key":"2023053020245725100_JOCES_17_3_567C3","doi-asserted-by":"publisher","first-page":"1998","DOI":"10.1073\/pnas.70.7.1998","article-title":"Restoration of hypoxanthine phosphoribosyl transferase activity in mouse iR cells after fusion with chick-embryo fibroblasts","volume":"70","author":"Bakay","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C4","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1007\/BF00485478","article-title":"An improved technique for the separation of glucose-6-phosphate dehydrogenase isoenzymes by disc electrophoresis on polyacrylamide gel","volume":"3","author":"Bakay","year":"1969","journal-title":"Biochem. Genet"},{"key":"2023053020245725100_JOCES_17_3_567C5","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/BF00485733","article-title":"The separation of adenine and hypoxanthine-guanine phosphoribosyl transferases isoenzymes by disc gel electrophoresis","volume":"5","author":"Bakay","year":"1971","journal-title":"Biochem. Genet"},{"key":"2023053020245725100_JOCES_17_3_567C6","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1073\/pnas.69.9.2523","article-title":"Activation of variants of hypoxanthine-guanine phosphoribosyl transferase by the normal enzyme","volume":"69","author":"Bakay","year":"1972","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C7","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/BF00486398","article-title":"Electrophoretic properties of hypoxanthine-guanine phosphoribosyl transferase in erythrocytes of subjects with Lesch-Nyhan syndrome","volume":"6","author":"Bakay","year":"1972","journal-title":"Biochem. Genet"},{"key":"2023053020245725100_JOCES_17_3_567C8","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1056\/NEJM197308302890903","article-title":"In vitro effects of magnesium ions on mutant cells from patients with the Lesch-Nyhan syndrome","volume":"289","author":"Benke","year":"1973","journal-title":"Netv Engl. J. Med"},{"key":"2023053020245725100_JOCES_17_3_567C9","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1242\/jcs.13.3.841","article-title":"Correction of genetic defects in mammalian cells by the input of small amounts of foreign genetic material","volume":"12","author":"Boyd","year":"1973","journal-title":"J. Cell Sci"},{"key":"2023053020245725100_JOCES_17_3_567C10","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/0014-4827(70)90466-0","article-title":"Uptake of isolated metaphase chromosomes by mammalian cells in vitro","volume":"61","author":"Burkholder","year":"1970","journal-title":"Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C11","doi-asserted-by":"publisher","first-page":"2590","DOI":"10.1073\/pnas.70.9.2590","article-title":"Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids","volume":"70","author":"Croce","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C12","doi-asserted-by":"publisher","first-page":"3617","DOI":"10.1073\/pnas.70.12.3617","article-title":"Assignment of the T-antigen gene of Simian virus 40 to human chromosome C-7","volume":"70","author":"Croce","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C13","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/0014-4827(73)90470-9","article-title":"Unidirectional loss of human chromosomes in rat-human hybrids","volume":"79","author":"Croce","year":"1973","journal-title":"Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C14","doi-asserted-by":"publisher","first-page":"1953","DOI":"10.1073\/pnas.69.7.1953","article-title":"Effect of environmental pH on the efficiency of cellular hybridization","volume":"69","author":"Croce","year":"1972","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C15","doi-asserted-by":"publisher","first-page":"1912","DOI":"10.1073\/pnas.70.7.1912","article-title":"Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: suppression and reactivation of the viral enzyme","volume":"70","author":"Davidson","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C16","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/0014-4827(73)90466-7","article-title":"Lesch-Nyhan syndrome: incorporation of hypoxanthine in stimulated lymphocytes","volume":"79","author":"De Bruyn","year":"1973","journal-title":"Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C17","volume-title":"In Chromosomes Today","author":"De La Chapell","year":"1973"},{"key":"2023053020245725100_JOCES_17_3_567C18","doi-asserted-by":"publisher","first-page":"3273","DOI":"10.1073\/pnas.69.11.3273","article-title":"Genetics of somatic mammalian cells: demonstration of a human esterase activator gene linked to the AdeB gene","volume":"69","author":"Kao","year":"1972","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C19","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1073\/pnas.71.4.1398","article-title":"Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion","volume":"71","author":"Klinger","year":"1974","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C20","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/0014-4827(66)90558-1","article-title":"The use of drug-resistant markers to study the hybridization of mouse fibroblasts","volume":"41","author":"Littlefield","year":"1966","journal-title":"Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C21","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1073\/pnas.70.4.1258","article-title":"Transfer of genetic information by purified metaphase chromosomes","volume":"70","author":"Mcbride","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C22","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1038\/223358a0","article-title":"Genetic analysis with human-mouse somatic cell hybrids","volume":"233","author":"Nabholz","year":"1969","journal-title":"Nature, Land"},{"key":"2023053020245725100_JOCES_17_3_567C23","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1073\/pnas.67.2.976","article-title":"Interallelic complementation in hybrid cells derived from human diploid strains deficient in galactose-i-phosphate uridyl transferase activity","volume":"67","author":"Nadler","year":"1970","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C24","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1073\/pnas.65.1.214","article-title":"Hemizygous expression of glucose-6-phosphate dehydrogenase in erythrocytes of heterozygotes for the Lesch-Nyhan syndrome","volume":"65","author":"Nyhan","year":"1970","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C25","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1038\/newbio241180a0","article-title":"Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci in man by somatic cell genetics","volume":"241","author":"Ricciuti","year":"1973","journal-title":"Nature, New Biol"},{"key":"2023053020245725100_JOCES_17_3_567C26","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/BF00487447","article-title":"Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases","volume":"9","author":"Roy","year":"1973","journal-title":"Biochem. Genet"},{"key":"2023053020245725100_JOCES_17_3_567C27","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1073\/pnas.68.7.1461","article-title":"Purification of IMP: pyrophosphate phosphoribosyltransferases, catalytically incompetent enzymes in Lesch-Nyhan disease","volume":"68","author":"Rubin","year":"1971","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C28","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1073\/pnas.68.1.127","article-title":"Expression of differentiated functions in hepatoma cell hybrids","volume":"68","author":"Schneider","year":"1971","journal-title":"I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids. Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C29","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1038\/newbio230005a0","article-title":"Correction of a genetic defect in a mammalian cell","volume":"230","author":"Schwartz","year":"1971","journal-title":"Nature, New Biol"},{"key":"2023053020245725100_JOCES_17_3_567C30","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1016\/S0140-6736(71)90287-X","article-title":"A rapid banding technique for human chromosomes","volume":"11","author":"Seabricht","year":"1971","journal-title":"Lancet"},{"key":"2023053020245725100_JOCES_17_3_567C31","first-page":"391","volume":"77","author":"Sekiguchi","year":"1973","journal-title":"Interallelic complementation in hybrid cells derived from Chinese hamster diploid clones deficient in hypoxanthine-guanine phosphoribosyl-transferase activity, Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C32","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1242\/jcs.14.2.235","article-title":"Nature of mutations conferring resistance to 8-azaguanine in mouse cell lines","volume":"14","author":"Shin","year":"1974","journal-title":"J. Cell Sci"},{"key":"2023053020245725100_JOCES_17_3_567C33","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1038\/newbio241194a0","article-title":"Cells with phosphoribosyl transferase activity recovered from mouse cells resistant to 8-azagua-nine","volume":"241","author":"Shin","year":"1973","journal-title":"Nature, New Biol"},{"key":"2023053020245725100_JOCES_17_3_567C34","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1007\/BF00485474","article-title":"Evolutionary evidence for a regulator gene controlling the lactate dehydrogenase B gene in rodent erythrocytes","volume":"8","author":"Shows","year":"1969","journal-title":"Biochem. Genet"},{"key":"2023053020245725100_JOCES_17_3_567C35","first-page":"75","article-title":"Genetic studies with human cell lines","volume":"7","author":"Szybalski","year":"1963","journal-title":"Natn. Cancer Inst. Monogr"},{"key":"2023053020245725100_JOCES_17_3_567C36","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/250580a0","article-title":"Genetic complementation after fusion of Tay-Sachs and Sandhoff cells","volume":"250","author":"Thomas","year":"1974","journal-title":"Nature, Land"},{"key":"2023053020245725100_JOCES_17_3_567C37","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1073\/pnas.71.1.45","article-title":"Assignment of the gene for adenine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization","volume":"71","author":"Tischfield","year":"1974","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C38","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1016\/0014-4827(72)90446-6","article-title":"Reappearance of murine hypoxanthine guanine phosphoribosyl transferase activity in mouse A9 cells after attempted hybridization with human cell lines","volume":"75","author":"Watson","year":"1972","journal-title":"Expl Cell Res"},{"key":"2023053020245725100_JOCES_17_3_567C39","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1073\/pnas.58.3.1104","article-title":"Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes","volume":"58","author":"Weiss","year":"1967","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020245725100_JOCES_17_3_567C40","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/newbio234020a0","article-title":"Loss of human genetic markers in man-Chinese hamster somatic cell hybrids","volume":"234","author":"Westerveld","year":"1971","journal-title":"Nature, New Biol"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/17\/3\/567\/2882480\/joces_17_3_567.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/17\/3\/567\/2882480\/joces_17_3_567.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T20:25:09Z","timestamp":1685478309000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/17\/3\/567\/57686\/Reversion-in-expression-of-hypoxanthine-guanine"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,5,1]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1975,5,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.17.3.567","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,5,1]]}}}