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This interaction has been further shown to facilitate the interdependent recruitment of Rap1 and SLAT to the T cell immunological synapse upon TCR engagement. Furthermore, a SLAT mutant lacking its PH domain drastically inhibited LFA-1 activation and CD4+ T cell adhesion. Finally, we established that a constitutively active form of Rap1, which is present at the plasma membrane, rescues the defective LFA-1 activation and ICAM-1 adhesion in SLAT-deficient (Def6\u2212\/\u2212) T cells. These findings ascribe a novel function to SLAT, and identify Rap1 as a target of SLAT function in TCR-mediated inside-out signaling.<\/jats:p>","DOI":"10.1242\/jcs.172742","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T00:35:13Z","timestamp":1445301313000},"source":"Crossref","is-referenced-by-count":5,"title":["SLAT promotes TCR-mediated, Rap1-dependent LFA-1 activation and adhesion through interaction of its PH domain with Rap1"],"prefix":"10.1242","author":[{"given":"Marjorie","family":"C\u00f4te","sequence":"first","affiliation":[{"name":"Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Fos","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann J.","family":"Canonigo-Balancio","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Ley","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"B\u00e9cart","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amnon","family":"Altman","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2015,1,1]]},"reference":[{"key":"2021042614475549000_JCS172742C1","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1146\/annurev.immunol.021908.132554","article-title":"The ins and outs of leukocyte integrin signaling","volume":"27","author":"Abram","year":"2009","journal-title":"Annu. 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