{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:52:24Z","timestamp":1788522744482,"version":"build-2803163510"},"reference-count":43,"publisher":"The Company of Biologists","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Eukaryotic initiation factor 6 (eIF6) is a pivotal regulator of ribosomal function, participating in translational control. Previously our data suggested that eIF6 acts as a key binding protein of P311 (a hypertrophic scar-related protein; also known as NREP). However, a comprehensive investigation of its functional role and the underlying mechanisms in modulation of myofibroblast (a key effector of hypertrophic scar formation) differentiation remains unclear. Here, we identified that eIF6 is a novel regulator of transforming growth factor-\u03b21 (TGF-\u03b21) expression at transcription level, which plays a key role in myofibroblast differentiation. Mechanistically, this effect is associated with eIF6 altering the occupancy of the TGF-\u03b21 promoter by H2A.Z (Swiss-Prot P0C0S6) and Sp1. Accordingly, modulation of eIF6 expression in myofibroblasts signi\ufb01cantly affects their differentiation via the TGF-\u03b2\/Smad signaling pathway, which was verified in vivo by the observation that heterozygote eIF6+\/\u2212 mice exhibited enhanced TGF-\u03b21 production coupled with increased \u03b1-smooth muscle actin (\u03b1-SMA)+ myofibroblasts after skin injury. Overall, our data reveal a novel transcriptional regulatory mechanism of eIF6 that acts on facilitating Sp1 recruitment to TGF-\u03b21 promoter via H2A.Z depletion and thus results in increased TGF-\u03b21 transcription, which contributes to myofibroblast differentiation.<\/jats:p>","DOI":"10.1242\/jcs.174870","type":"journal-article","created":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T00:48:07Z","timestamp":1442969287000},"page":"3977-3989","source":"Crossref","is-referenced-by-count":36,"title":["Eukaryotic initiation factor 6 modulates myofibroblast differentiation at transforming growth factor-\u03b21 transcription level via H2A.Z occupancy and Sp1 recruitment"],"prefix":"10.1242","volume":"128","author":[{"given":"Si-si","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Burn Research 1 , State Key Laboratory of Trauma, Burn and Combined Injury , , Chongqing 400038, People's Republic of \u2008 China"},{"name":"Southwest Hospital, The Third Military 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