{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:01:16Z","timestamp":1791226876811,"version":"4.1.0"},"reference-count":30,"publisher":"The Company of Biologists","issue":"14","license":[{"start":{"date-parts":[[2017,1,15]],"date-time":"2017-01-15T00:00:00Z","timestamp":1484438400000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.biologists.com\/user-licence-1-1"}],"funder":[{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Rif induces dorsal filopodia but the signaling pathway responsible for this has not been identified. We show here that Rif interacts with the I-BAR family protein IRTKS (also known as BAIAP2L1) through its I-BAR domain. Rif also interacts with Pinkbar (also known as BAIAP2L2) in N1E-115 mouse neuroblastoma cells. IRTKS and Rif induce dorsal membrane ruffles and filopodia. Dominant-negative Rif inhibits the formation of IRTKS-induced morphological structures, and Rif activity is blocked in IRTKS-knockout (KO) cells. To further define the Rif\u2013IRTKS signaling pathway, we identify Eps8 and WAVE2 (also known as WASF2) as IRTKS interactors. We find that Eps8 regulates the size and number of dorsal filopodia and membrane ruffles downstream of Rif\u2013IRTKS signaling, whereas WAVE2 modulates dorsal membrane ruffling. Furthermore, our data suggests that Tir, a protein essential for enterohemorrhagic Escherichia coli infection, might compete for Rif for interaction with the I-BAR domain of IRTKS. Based on this evidence, we propose a model in which Rho family GTPases use the I-BAR proteins, IRSp53 (also known as BAIAP2), IRTKS and Pinkbar, as a central mechanism to modulate cell morphology.<\/jats:p>","DOI":"10.1242\/jcs.179655","type":"journal-article","created":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T00:39:04Z","timestamp":1465432744000},"page":"2829-2840","source":"Crossref","is-referenced-by-count":33,"title":["The Rho GTPase Rif signals through IRTKS, Eps8 and WAVE2 to generate dorsal membrane ruffles and filopodia"],"prefix":"10.1242","volume":"129","author":[{"given":"Thankiah","family":"Sudhaharan","sequence":"first","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Ping","family":"Sem","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwi Fen","family":"Liew","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan Hong","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wah Ing","family":"Goh","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"},{"name":"Mechanobiology Institute, National University of Singapore 2 , 5A Engineering Drive 1, 117411 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai Mei","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5208-8193","authenticated-orcid":false,"given":"Sohail","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology 1 Neural Stem Cell Laboratory , , 8A Biomedical Grove, Immunos, 138648 \u2008 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061316221102800_JCS179655C1","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.semcdb.2009.11.008","article-title":"I-BAR domains, IRSp53 and filopodium formation","volume":"21","author":"Ahmed","year":"2010","journal-title":"Semin. 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