{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:23:55Z","timestamp":1790263435405,"version":"4.1.0"},"reference-count":42,"publisher":"The Company of Biologists","issue":"11","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"vor","delay-in-days":183,"URL":"http:\/\/www.biologists.com\/user-licence-1-1"}],"funder":[{"name":"Fondo de Investigaciones Sanitarias Instituto Carlos III","award":["PI11\/00922"],"award-info":[{"award-number":["PI11\/00922"]}]},{"name":"Fundaci? per Amor a L?rt","award":["2014"],"award-info":[{"award-number":["2014"]}]},{"DOI":"10.13039\/100008054","name":"Fundaci?n Ram?n Areces","doi-asserted-by":"publisher","award":["2013"],"award-info":[{"award-number":["2013"]}],"id":[{"id":"10.13039\/100008054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The Cu+ pump ATP7B plays an irreplaceable role in the elimination of excess Cu+ by the hepatocyte into the bile. The trafficking and site of action of ATP7B are subjects of controversy. One current proposal is that an increase in intracellular Cu+ results in the translocation of ATP7B to the lysosomes and excretion of excess Cu+ through lysosomal-mediated exocytosis at the bile canaliculus. Here, we show that ATP7B is transported from the trans-Golgi network (TGN) to the bile canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu+ does not cause relocalization of lysosomes and the appearance of lysosome markers in the bile canaliculus. Our data reveal the pathway of the Cu+-mediated transport of ATP7B from the TGN to the bile canaliculus and indicates that the bile canaliculus is the primary site of ATP7B action in the elimination of excess Cu+.<\/jats:p>","DOI":"10.1242\/jcs.184663","type":"journal-article","created":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T01:20:13Z","timestamp":1459473613000},"page":"2190-2201","source":"Crossref","is-referenced-by-count":25,"title":["Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus"],"prefix":"10.1242","volume":"129","author":[{"given":"Vasiliki","family":"Lalioti","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 1 Department of Cell Biology and Immunology , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Peir\u00f3","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 2 Department of Genomics and Massive Sequencing , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8129-0107","authenticated-orcid":false,"given":"Manuela","family":"P\u00e9rez-Berlanga","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 1 Department of Cell Biology and Immunology , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yo","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 1 Department of Cell Biology and Immunology , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeles","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 3 Department of Optical and Confocal Microscopy , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Villalba","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 3 Department of Optical and Confocal Microscopy , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 3 Department of Optical and Confocal Microscopy , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1485-2936","authenticated-orcid":false,"given":"Ignacio V.","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Cantoblanco 1 Department of Cell Biology and Immunology , , Madrid 28049 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061316162069800_JCS184663C1","doi-asserted-by":"crossref","first-page":"16755","DOI":"10.1016\/S0021-9258(18)66630-X","article-title":"Biosynthesis, glycosylation, movement through the Golgi system, and transport to lysosomes by an N-linked carbohydrate-independent mechanism of three lysosomal integral membrane proteins","volume":"261","author":"Barriocanal","year":"1986","journal-title":"J. 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