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Although the FASII fatty acid biosynthesis pathway located in the apicoplast is essential for parasite survival, the cellular effects of FASII disruption in T. gondii had not been examined in detail. Here, we combined light and electron microscopy techniques \u2013 including focused ion beam scanning electron microscopy (FIB-SEM) \u2013 to characterize the effect of FASII disruption in T. gondii, by treatment with the FASII inhibitor triclosan or by inducible knockdown of the FASII component acyl carrier protein. Morphological analyses showed that FASII disruption prevented cytokinesis completion in T. gondii tachyzoites, leading to the formation of large masses of \u2018tethered\u2019 daughter cells. FIB-SEM showed that tethered daughters had a mature basal complex, but a defect in new membrane addition between daughters resulted in incomplete pellicle formation. Addition of exogenous fatty acids to medium suppressed the formation of tethered daughter cells and supports the notion that FASII is essential to generate lipid substrates required for the final step of parasite division.<\/jats:p>","DOI":"10.1242\/jcs.185223","type":"journal-article","created":{"date-parts":[[2016,7,26]],"date-time":"2016-07-26T02:03:51Z","timestamp":1469498631000},"page":"3320-3331","source":"Crossref","is-referenced-by-count":43,"title":["Apicoplast fatty acid synthesis is essential for pellicle formation at the end of cytokinesis in <i>Toxoplasma gondii<\/i>"],"prefix":"10.1242","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-6953","authenticated-orcid":false,"given":"\u00c9rica S.","family":"Martins-Duarte","sequence":"first","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 1 Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer , , Brazil , 21.941-902"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Bioimagens 2 , Rio de Janeiro , Brazil , 21.941-902"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maira","family":"Carias","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 1 Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer , , Brazil , 21.941-902"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Bioimagens 2 , Rio de Janeiro , Brazil , 21.941-902"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossiane","family":"Vommaro","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 1 Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer , , Brazil , 21.941-902"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Bioimagens 2 , Rio de Janeiro , Brazil , 21.941-902"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namita","family":"Surolia","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research 3 Molecular Biology and Genetics Unit , , Jakkur, Bangalore , India , 560064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanderley","family":"de Souza","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 1 Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer , , Brazil , 21.941-902"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Bioimagens 2 , Rio de Janeiro , Brazil , 21.941-902"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061316242083500_JCS185223C1","doi-asserted-by":"publisher","first-page":"e1000270","DOI":"10.1371\/journal.ppat.1000270","article-title":"Rab11A-controlled assembly of the inner membrane complex is required for completion of apicomplexan cytokinesis","volume":"5","author":"Agop-Nersesian","year":"2009","journal-title":"PLoS Pathog."},{"key":"2024061316242083500_JCS185223C2","doi-asserted-by":"publisher","first-page":"e1003426","DOI":"10.1371\/journal.ppat.1003426","article-title":"An apicoplast localized ubiquitylation system is required for the import of nuclear-encoded plastid proteins","volume":"9","author":"Agrawal","year":"2013","journal-title":"PLoS Pathog."},{"key":"2024061316242083500_JCS185223C3","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1111\/j.1462-5822.2010.01514.x","article-title":"A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii","volume":"13","author":"Anderson-White","year":"2011","journal-title":"Cell. 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