{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:55:20Z","timestamp":1791269720465,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","issue":"17","license":[{"start":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T00:00:00Z","timestamp":1599350400000},"content-version":"vor","delay-in-days":1466,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Correct innervation of the main respiratory muscle in mammals, namely the thoracic diaphragm, is a crucial pre-requisite for the functionality of this muscle and the viability of the entire organism. Systemic impairment of Sema3A\u2013Npn-1 (Npn-1 is also known as NRP1) signaling causes excessive branching of phrenic nerves in the diaphragm and into the central tendon region, where the majority of misguided axons innervate ectopic musculature. To elucidate whether these ectopic muscles are a result of misguidance of myoblast precursors due to the loss of Sema3A\u2013Npn-1 signaling, we conditionally ablated Npn-1 in somatic motor neurons, which led to a similar phenotype of phrenic nerve defasciculation and, intriguingly, also formation of innervated ectopic muscles. We therefore hypothesize that ectopic myocyte fusion is caused by additional factors released by misprojecting growth cones. Slit2 and its Robo receptors are expressed by phrenic motor axons and migrating myoblasts, respectively, during innervation of the diaphragm. In vitro analyses revealed a chemoattractant effect of Slit2 on primary diaphragm myoblasts. Thus, we postulate that factors released by motor neuron growth cones have an influence on the migration properties of myoblasts during establishment of the diaphragm.<\/jats:p>","DOI":"10.1242\/jcs.186015","type":"journal-article","created":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T01:40:12Z","timestamp":1469670012000},"page":"3295-3308","source":"Crossref","is-referenced-by-count":14,"title":["The role of Sema3\u2013Npn-1 signaling during diaphragm innervation and muscle development"],"prefix":"10.1242","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8774-7892","authenticated-orcid":false,"given":"Maximilian Michael","family":"Saller","sequence":"first","affiliation":[{"name":"Ludwig-Maximilians-University (LMU) 1 Experimental Surgery and Regenerative Medicine, Department of Surgery , , Nu\u00dfbaumstra\u00dfe 20, Munich 80336 , Germany"},{"name":"Institute of Developmental Genetics, Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health (GmbH) 2 , Ingolst\u00e4dter Landstra\u00dfe 1, Neuherberg 85764 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa-Eva","family":"Huettl","sequence":"additional","affiliation":[{"name":"Institute of Developmental Genetics, Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health (GmbH) 2 , Ingolst\u00e4dter Landstra\u00dfe 1, Neuherberg 85764 , Germany"},{"name":"Institute of Physiology 3 , Department of Physiological Genomics , , Schillerstra\u00dfe 46, Munich 80336 , Germany"},{"name":"Ludwig-Maximilians-University (LMU) 3 , Department of Physiological Genomics , , Schillerstra\u00dfe 46, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Hanuschick","sequence":"additional","affiliation":[{"name":"Institute of Developmental Genetics, Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health (GmbH) 2 , Ingolst\u00e4dter Landstra\u00dfe 1, Neuherberg 85764 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Lena","family":"Amend","sequence":"additional","affiliation":[{"name":"Institute of Developmental Genetics, Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health (GmbH) 2 , Ingolst\u00e4dter Landstra\u00dfe 1, Neuherberg 85764 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Alberton","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-University (LMU) 1 Experimental Surgery and Regenerative Medicine, Department of Surgery , , Nu\u00dfbaumstra\u00dfe 20, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Aszodi","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilians-University (LMU) 1 Experimental Surgery and Regenerative Medicine, Department of Surgery , , Nu\u00dfbaumstra\u00dfe 20, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea B.","family":"Huber","sequence":"additional","affiliation":[{"name":"Institute of Developmental Genetics, Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health (GmbH) 2 , Ingolst\u00e4dter Landstra\u00dfe 1, Neuherberg 85764 , Germany"},{"name":"Bernstein Network for Computational Neuroscience, Albert-Ludwigs-University 4 , Freiburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061316240292100_JCS186015C2","doi-asserted-by":"publisher","first-page":"L1310","DOI":"10.1152\/ajplung.00257.2002","article-title":"Diaphragm defects occur in a CDH hernia model independently of myogenesis and lung formation","volume":"283","author":"Babiuk","year":"2002","journal-title":"Am. 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