{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:32:12Z","timestamp":1790980332638,"version":"4.1.0"},"reference-count":31,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1975,10,1]],"date-time":"1975-10-01T00:00:00Z","timestamp":181353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1975,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Cellular uptake of ferritin amounting to 0\u00b75 \u03bcg\/mg cell protein or more can be measured colorimetrically on the basis of ferritin-iron content. 131I-serum albumin, soluble ferritin and aggregated ferritin used in equimolar concentrations are taken up differently by Sarcoma S180 cells in culture. The net uptakes in 2 h at 37 \u00b0C are 0\u00b7065, 4\u00b73 and 24\u00b77 \u03bcg I mg cell protein or 0\u00b793, 8\u00b70 and 45\u00b77 \u03bcmol, respectively. Albumin uptake is not inhibited by a 26-fold molar ferritin excess but is significantly inhibited by a 43-fold excess.<\/jats:p>\n               <jats:p>The transport mechanism of the ferritins differs from that of albumin in that it is significantly inhibitable by 2 \u00d7 10\u22121 M monoiodoacetate. Soluble ferritin contains small aggregates which are removed by filtration through Millipore membranes of 0\u00b705, 01 and 0\u00b722 \u03bcm. When the 0\u00b71\u03bcm filtrate is re-examined, uptake is no longer inhibited by iodoacetate. Since it can be inferred from other work that albumin is taken up by pinocytosis and ferritin aggregates by phagocytosis, the difference in susceptibility to inhibition is proposed as a way to distinguish pinocytosis from phagocytosis.<\/jats:p>\n               <jats:p>Ferritin may form larger visible aggregates in culture medium. The transport mechanism of this aggregated ferritin differs from that of soluble unfiltered ferritin in that it causes concomitant enhancement of albumin uptake. Albumin transported by virtue of this effect becomes partially susceptible to iodoacetate. Thus, in addition to a distinction between pinocytosis and phagocytosis, our data single out 2 forms of albumin transport and 3 forms of ferritin transport.<\/jats:p>","DOI":"10.1242\/jcs.19.1.141","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:17:11Z","timestamp":1619482631000},"page":"141-156","source":"Crossref","is-referenced-by-count":18,"title":["Cellular uptake of soluble and aggregated ferritin: Distinction between pinocytosis and phagocytosis"],"prefix":"10.1242","volume":"19","author":[{"given":"M.-P.","family":"Petitpierre-Gabathuler","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Harvard Medical School, Boston, Massachusetts"},{"name":"Boston University School of Medicine ** Department of Pathology , , 80 East Concord Street, Boston, Massachusetts 02118, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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