{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T12:39:46Z","timestamp":1771072786978,"version":"3.50.1"},"reference-count":46,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24106508"],"award-info":[{"award-number":["JP24106508"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP26106718"],"award-info":[{"award-number":["JP26106718"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The importance of macrophages in tissue development and regeneration has been strongly emphasized. However, the specific roles of macrophage colony-stimulating factor (MCSF), the key regulator of macrophage differentiation, in glandular tissue development have been unexplored. Here, we disclose new macrophage-independent roles of MCSF in tissue development. We initially found that MCSF is markedly upregulated at embryonic day (E)13.5, at a stage preceding the colonization of macrophages (at E15.5), in mouse submandibular gland (SMG) tissue. Surprisingly, MCSF-induced branching morphogenesis was based on a direct effect on epithelial cells, as well as indirectly, by modulating the expression of major growth factors of SMG growth, FGF7 and FGF10, via the phosphoinositide 3-kinase (PI3K) pathway. Additionally, given the importance of neurons in SMG organogenesis, we found that MCSF-induced SMG growth was associated with regulation of neurturin expression and neuronal network development during early SMG development in an in vitro organogenesis model as well as in vivo. These results indicate that MCSF plays pleiotropic roles and is an important regulator of early SMG morphogenesis.<\/jats:p>","DOI":"10.1242\/jcs.196907","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T00:10:42Z","timestamp":1490659842000},"page":"1559-1569","source":"Crossref","is-referenced-by-count":25,"title":["MCSF orchestrates branching morphogenesis in developing submandibular gland tissue"],"prefix":"10.1242","volume":"130","author":[{"given":"Gulsan Ara","family":"Sathi","sequence":"first","affiliation":[{"name":"Okayama University 1 Department of Biomaterials , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Farahat","sequence":"additional","affiliation":[{"name":"Okayama University 1 Department of Biomaterials , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"},{"name":"Okayama University 2 Department of Oral Rehabilitation and Regenerative Medicine , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio Satoshi","family":"Hara","sequence":"additional","affiliation":[{"name":"Okayama University 1 Department of Biomaterials , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Taketa","sequence":"additional","affiliation":[{"name":"Center for the Development of Medical and Health Care Education, Okayama University 3 , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Nagatsuka","sequence":"additional","affiliation":[{"name":"Okayama University 4 Department of Oral Pathology and Medicine , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuo","family":"Kuboki","sequence":"additional","affiliation":[{"name":"Okayama University 2 Department of Oral Rehabilitation and Regenerative Medicine , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9804-4786","authenticated-orcid":false,"given":"Takuya","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Okayama University 1 Department of Biomaterials , , 2-5-1 Shikata-Cho, Okayama 700-8558 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2024061317172576900_JCS196907C1","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1189\/jlb.1103591","article-title":"Insulin cell mass is altered in Csf1op\/Csf1op macrophage-deficient mice","volume":"76","author":"Banaei-Bouchareb","year":"2004","journal-title":"J. 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