{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T04:18:47Z","timestamp":1770437927433,"version":"3.49.0"},"reference-count":35,"publisher":"The Company of Biologists","issue":"Supplement_6","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Human cells that lack O1-alkylguanine DNA alkyltransferase (AT) activity can remove O1-butylguanine (O1-wBuG) produced in cellular DNA by exposure to N-n-butyl-N-nitrosourea as determined by radioimmunoassay of enzyme digests of DNA. Fibroblasts from xeroderma pigmentosum (XP) complementation groups A and G that show &amp;lt;5 % unscheduled DNA synthesis following exposure to UVC failed to remove O1-nBuG. Hence it appears that O1- alkylguanine is repaired in cells that lack AT by a process that is defective in XP cells, presumably nucleotide excision repair. Neither V79 nor V79\/79 Chinese hamster cell lines have AT activity and both are able to remove O1-nBuG from DNA. However, only V79\/79 is able to remove O1MeG, suggesting some substrate specificity of the excision repair process. Comparison of relative levels of O1-alkylation by N-mcthyl-, N-ethyl-, N-propyl- and N-n-butyl-nitrosourea indicate that approximately equal levels of O1-alkylation are produced by equitoxic doses of these agents.<\/jats:p>","DOI":"10.1242\/jcs.1984.supplement_6.10","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T07:59:48Z","timestamp":1369295988000},"page":"147-160","source":"Crossref","is-referenced-by-count":14,"title":["Genetic Evidence for Nucleotide Excision Repair of O6-Alkylguanine in Mammalian Cells"],"prefix":"10.1242","volume":"1987","author":[{"given":"J. M.","family":"Boyle","sequence":"first","affiliation":[{"name":"Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. G.","family":"Durrant","sequence":"additional","affiliation":[{"name":"Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. P.","family":"Wild","sequence":"additional","affiliation":[{"name":"Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Saffhill","sequence":"additional","affiliation":[{"name":"Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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