{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:34:42Z","timestamp":1760783682332},"reference-count":30,"publisher":"The Company of Biologists","issue":"Supplement_6","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>A re-analysis of the metabolic fate of ultraviolet light (u.v.)-induced cyclobutyl pyrimidine dimers in the DNA of dermal fibroblasts from patients with different genetic forms of xeroderma pigmentosum (XP), a rare cancer-prone skin disorder, has provided new insight into the mode of dimer repair in normal human cells. When DNA isolated from post-u.v. incubated cultures was subjected to enzymic photoreactivation (PR) to probe dimer authenticity, single-strand scissions were produced in the damaged DNA of incubated XP group A and D cells, but not in DNA from XP group C cells or normal controls. Since enzymic PR treatment ruptures only the cyclobutane ring, these results suggested that in dimer excision-defective XP group A and D strains, the intradimer phosphodiester bond may have been cleaved without site restoration. Such a cleavage event had not previously been detected; the possibility that this reaction may be an early step in the normal excision-repair process is supported by the observed release of free thymidine (dThd) and its monophosphate (TMP), but not of thymine, upon photochemical reversal of the dimercontaining excision fragments isolated from post-u.v. incubated normal cells. The combined number of dThd and TMP molecules released was equal to \u2248 80% of the number of dimers photoreversed; for such release to occur, the dimer must both be at one end of an excised fragment and contain an internal phosphodiester break. Taken together, these data lead us to propose a novel model for dimer repair in human cells in which hydrolysis of the intradimer phosphodiester linkage precedes the concerted action of a generalized \u2018bulky lesion-repair complex\u2019 involving conventional strand incision\/lesion excision\/repair resynthesis\/strand ligation reactions.<\/jats:p>","DOI":"10.1242\/jcs.1984.supplement_6.11","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T07:59:48Z","timestamp":1369295988000},"page":"161-176","source":"Crossref","is-referenced-by-count":18,"title":["Molecular Evidence for Cleavage of Intradimer Phosphodiester Linkage as a Novel Step in Excision Repair of Cyclobutyl Pyrimidine Photodimers in Cultured Human Cells"],"prefix":"10.1242","volume":"1987","author":[{"given":"M. C.","family":"Paterson","sequence":"first","affiliation":[{"name":"1 Molecular Genetics and Carcinogenesis Laboratory, Department of Medicine, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada"},{"name":"2 Health Sciences Division, Chalk Biver Nuclear Laboratories, Chalk River, Ontario KOJ 1J0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Middlestadt","sequence":"additional","affiliation":[{"name":"1 Molecular Genetics and Carcinogenesis Laboratory, Department of Medicine, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada"},{"name":"2 Health Sciences Division, Chalk Biver Nuclear Laboratories, Chalk River, Ontario KOJ 1J0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. J.","family":"Macfarlane","sequence":"additional","affiliation":[{"name":"1 Molecular Genetics and Carcinogenesis Laboratory, Department of Medicine, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. E.","family":"Gentner","sequence":"additional","affiliation":[{"name":"2 Health Sciences Division, Chalk Biver Nuclear Laboratories, Chalk River, Ontario KOJ 1J0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Weinfeld","sequence":"additional","affiliation":[{"name":"1 Molecular Genetics and Carcinogenesis Laboratory, Department of Medicine, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada"},{"name":"2 Health Sciences Division, Chalk Biver Nuclear Laboratories, Chalk River, Ontario KOJ 1J0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P. M.","family":"Eker","sequence":"additional","affiliation":[{"name":"1 Molecular Genetics and Carcinogenesis Laboratory, Department of Medicine, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1987,1,1]]},"reference":[{"key":"2024071019355321900_JOCES_1987_S6_161C1","first-page":"53","article-title":"DNA damage, repair systems and human hypersensitive diseases","volume":"3","author":"Cleaver","year":"1980","journal-title":"J. environ. Path. Toxicol."},{"key":"2024071019355321900_JOCES_1987_S6_161C2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/1523-1747.ep12260596","article-title":"Repair of psoralen adducts in human DNA: Differences among xeroderma pigmentosum complementation groups","volume":"82","author":"Cleaver","year":"1984","journal-title":"J. invest. Derm."},{"key":"2024071019355321900_JOCES_1987_S6_161C3","first-page":"4579","volume":"251","author":"Doniger","year":"1976","journal-title":"Human correxonuclease. Purification and properties of a DNA repair exonuclease from placenta..J, biol. Chem."},{"key":"2024071019355321900_JOCES_1987_S6_161C4","first-page":"141","volume-title":"DNARepair,","author":"Friedberg","year":"1985"},{"key":"2024071019355321900_JOCES_1987_S6_161C5","first-page":"263","article-title":"DNA repair enzymes in mammalian cells","volume":"2","author":"Friedberg","year":"1977","journal-title":"Photochem. Photobiol. Rev."},{"key":"2024071019355321900_JOCES_1987_S6_161C6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/B978-0-12-035408-5.50008-3","article-title":"Human diseases associated with defective DNA repair","volume":"8","author":"Friedberg","year":"1979","journal-title":"Adv. Radiat. 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Mutat. Res."},{"key":"2024071019355321900_JOCES_1987_S6_161C11","first-page":"113","volume-title":"Update: Dermatology in General Medicine","author":"Kraemer","year":"1983"},{"key":"2024071019355321900_JOCES_1987_S6_161C12","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1073\/pnas.72.1.59","article-title":"Genetic heterogeneity in xeroderma pigmentosum: Complementation groups and their relationship to DNA repair rates","volume":"72","author":"Kraemer","year":"1975","journal-title":"Proc. natn. Acad. Sci. U.S.A."},{"key":"2024071019355321900_JOCES_1987_S6_161C13","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0027-5107(79)90230-6","article-title":"Survival of apurinic SV40 DNA in the D complementation group of xeroderma pigmentosum","volume":"62","author":"Kudrna","year":"1979","journal-title":"Mutat. 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