{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:49:40Z","timestamp":1779342580999,"version":"3.51.4"},"reference-count":35,"publisher":"The Company of Biologists","issue":"Supplement_6","license":[{"start":{"date-parts":[[1987,1,1]],"date-time":"1987-01-01T00:00:00Z","timestamp":536457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We have utilized DNA transfer and recombinant DNA techniques to probe DNA double-strand break repair in the human ionizing radiation-sensitive genetic syndrome ataxia-telangiectasia (A-T). Using restriction enzyme-generated double-strand breaks in the coding sequence of a selectable gene we have detected a significantly greater frequency of mis-repair of such breaks in a permanent A-T cell line compared with cell lines of normal radiosensitivity. This mis-repair in A-T can plausibly explain many of the clinical features of the disease but was insufficiently detailed to address the broad problem of DNA repair mechanisms relevant to ionizing radiation-induced damage. To extend these observations of DNA double-strand break mis-repair we have now applied this type of repair assay to novel, de novo induced mammalian X-ray-sensitive cell lines and to appropriate Escherichia coli mutants. In both cellular systems we have now found some equivalence to the A-T repair defect. In particular, studies on one E. coli mutant have provided evidence suggesting an involvement of a topoisomerase activity in DNA double-strand break misrepair, which may be relevant to the biochemical defect in A-T.<\/jats:p>","DOI":"10.1242\/jcs.1984.supplement_6.12","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T07:59:48Z","timestamp":1369295988000},"page":"177-189","source":"Crossref","is-referenced-by-count":40,"title":["Molecular Studies on the Nature of the Repair Defect in Ataxia-Telangiectasia and Their Implications for Cellular Radiobiology"],"prefix":"10.1242","volume":"1987","author":[{"given":"P. G.","family":"Debenham","sequence":"first","affiliation":[{"name":"Division of Cell and Molecular Biology, MRC Radiobiology Unit, Chilton, Oxon, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. B. T.","family":"Webb","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, MRC Radiobiology Unit, Chilton, Oxon, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. J.","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, MRC Radiobiology Unit, Chilton, Oxon, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Cox","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, MRC Radiobiology Unit, Chilton, Oxon, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1987,1,1]]},"reference":[{"key":"2024071019353966400_JOCES_1987_S6_177C1","volume-title":"Ataxia-telangiectasia. 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Biol."},{"key":"2024071019353966400_JOCES_1987_S6_177C9","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/287745a0","article-title":"Unusual levels of (ADP-ribose)n and DNA synthesis in ataxia-telangiectasia cells following y-irradiation","volume":"287","author":"Edwards","year":"1980","journal-title":"Nature, Lond."},{"key":"2024071019353966400_JOCES_1987_S6_177C10","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1146\/annurev.bi.50.070181.004311","article-title":"DNA topoisomerases. A","volume":"50","author":"Gellert","year":"1981","journal-title":"Rev. Biochem."},{"key":"2024071019353966400_JOCES_1987_S6_177C11","doi-asserted-by":"crossref","first-page":"4772","DOI":"10.1073\/pnas.74.11.4772","article-title":"Nalidixic acid resistance: A second genetic character involved in DNA gyrase activity","volume":"74","author":"Gellert","year":"1977","journal-title":"Proc. natn. Acad. Sci. 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Acids Res."},{"key":"2024071019353966400_JOCES_1987_S6_177C15","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.1093\/nar\/8.16.3709","article-title":"Effect of ionising radiation on DNA synthesis in ataxiatelangiectasia","volume":"8","author":"Houldsworth","year":"1980","journal-title":"Nucl. Acids Res."},{"key":"2024071019353966400_JOCES_1987_S6_177C16","first-page":"313","article-title":"X-ray sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents","volume":"112","author":"Jeggo","year":"1983","journal-title":"Mutat. Res."},{"key":"2024071019353966400_JOCES_1987_S6_177C17","first-page":"189","article-title":"X-ray sensitive mutants of Chinese hamster ovary cells defective in double-strand break rejoining","volume":"132","author":"Kemp","year":"1984","journal-title":"Mutat. 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