{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:42:02Z","timestamp":1770975722935,"version":"3.50.1"},"reference-count":8,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1976,1,1]],"date-time":"1976-01-01T00:00:00Z","timestamp":189302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1976,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Patients with Xeroderma pigmentosum and defective DNA excision repair can be distinguished as a rapid (r-XP) and slow (s-XP) complementing variety. When fused with normal cells, fibroblasts from the r-XP are complemented rapidly and in the absence of protein synthesis while those from the s-XP are complemented slowly by a process partly, but not entirely, dependent on protein synthesis. Heterokaryons with different ratios of r-XP to s-XP nuclei (i.e. 1:1\u20135 and 1\u20135:1) and control heterokaryons containing one normal and 1\u20135 r- or s-XP nuclei show that if cell fusion and incubation is conducted in medium preventing protein synthesis, the r-XP cells do not complement the 8-XP partner at all and, conversely, that the latter is not as effective as normal cells at complementing the r-XP partner. On the contrary, if protein synthesis is permitted, the 2 types of XP cells complement each other in a gene dose-dependent manner and to an extent similar to that observed in the control heterokaryons. These findings indicate that the r- and 8-XP varieties are caused by mutations at different loci and suggest that the products of these loci interact to produce a functional unit which is present in normal control cells but absent in the XP strains. The relationship between the complementation groups described here and those already reported in the literature is being investigated.<\/jats:p>","DOI":"10.1242\/jcs.20.1.207","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T05:16:47Z","timestamp":1619241407000},"page":"207-213","source":"Crossref","is-referenced-by-count":19,"title":["DNA repair synthesis in human heterokaryons: III. the rapid and slow complementing varieties of xeroderma pigmentosum"],"prefix":"10.1242","volume":"20","author":[{"given":"F.","family":"Giannelli","sequence":"first","affiliation":[{"name":"Paediatric Research Unit, Prince Philip Research Laboratories, Guy\u2019s Hospital Medical School , London SEI 9RT, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. A.","family":"Pawsey","sequence":"additional","affiliation":[{"name":"Paediatric Research Unit, Prince Philip Research Laboratories, Guy\u2019s Hospital Medical School , London SEI 9RT, England"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1976,1,1]]},"reference":[{"key":"2023053020264939200_JOCES_20_1_207C1","volume-title":"In Molecular Mechanisms for the Repair of DNA","author":"Bootsma","year":"1975"},{"key":"2023053020264939200_JOCES_20_1_207C2","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/256235a0","article-title":"Excision of thymine dimers from specifically incised DNA by extracts of Xeroderma pigmentosum cells","volume":"256","author":"Cook","year":"1975","journal-title":"Nature, Land"},{"key":"2023053020264939200_JOCES_20_1_207C3","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/0014-4827(73)90052-9","article-title":"DNA repair synthesis in human heterokaryons formed by normal and UV-sensitive fibroblasts","volume":"78","author":"Giannelli","year":"1973","journal-title":"Expl Cell Res"},{"key":"2023053020264939200_JOCES_20_1_207C4","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1242\/jcs.15.1.163","article-title":"DNA repair synthesis in human heterokaryons. II. A test for heterozygosity in Xeroderma pigmentosum and some insight into the structure of the defective enzyme","volume":"15","author":"Giannelli","year":"1974","journal-title":"J. Cell Sci"},{"key":"2023053020264939200_JOCES_20_1_207C5","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1038\/205640a0","article-title":"Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species","volume":"205","author":"Harris","year":"1965","journal-title":"Nature, Loud"},{"key":"2023053020264939200_JOCES_20_1_207C6","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1073\/pnas.72.1.59","article-title":"Genetic heterogeneity in Xeroderma pigmentosum: complementation groups and their relationship to DNA repair rates","volume":"72","author":"Kraemer","year":"1975","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053020264939200_JOCES_20_1_207C7","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1073\/pnas.72.1.219","article-title":"Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation","volume":"72","author":"Lehmann","year":"1975","journal-title":"Proc, natn. Acad. Sci. U.S.A"},{"key":"2023053020264939200_JOCES_20_1_207C8","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/0027-5107(73)90083-3","article-title":"Use of a UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cells","volume":"19","author":"Paterson","year":"1973","journal-title":"Mutation Res"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/20\/1\/207\/2883556\/joces_20_1_207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/20\/1\/207\/2883556\/joces_20_1_207.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T20:26:54Z","timestamp":1685478414000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/20\/1\/207\/58136\/DNA-repair-synthesis-in-human-heterokaryons-III"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,1,1]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1976,1,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.20.1.207","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,1,1]]}}}