{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:29:44Z","timestamp":1776259784120,"version":"3.50.1"},"reference-count":36,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T00:00:00Z","timestamp":1515974400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16J02257"],"award-info":[{"award-number":["16J02257"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K14541"],"award-info":[{"award-number":["15K14541"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H06465"],"award-info":[{"award-number":["16H06465"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H06173"],"award-info":[{"award-number":["16H06173"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H05846"],"award-info":[{"award-number":["17H05846"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cell fusion is a pivotal process in fertilization and multinucleate cell formation. A plant cell is ubiquitously surrounded by a hard cell wall, and very few cell fusions have been observed except for gamete fusions. We recently reported that the fertilized central cell (the endosperm) absorbs the persistent synergid, a highly differentiated cell necessary for pollen tube attraction. The synergid\u2013endosperm fusion (SE fusion) appears to eliminate the persistent synergid from fertilized ovule in Arabidopsis thaliana. Here, we analyzed the effects of various inhibitors on SE fusion in an in vitro culture system. Different from other cell fusions, neither disruption of actin polymerization nor protein secretion impaired SE fusion. However, transcriptional and translational inhibitors decreased the SE fusion success rate and also inhibited endosperm division. Failures of SE fusion and endosperm nuclear proliferation were also induced by roscovitine, an inhibitor of cyclin-dependent kinases (CDK). These data indicate unique aspects of SE fusion such as independence of filamentous actin support and the importance of CDK-mediated mitotic control.<\/jats:p>","DOI":"10.1242\/jcs.204123","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T20:10:17Z","timestamp":1502741417000},"source":"Crossref","is-referenced-by-count":4,"title":["A pharmacological study of\n                    <i>Arabidopsis<\/i>\n                    cell fusion between the persistent synergid and endosperm"],"prefix":"10.1242","volume":"131","author":[{"given":"Kazuki","family":"Motomura","sequence":"first","affiliation":[{"name":"Institute of Transformative Bio-Molecules (ITbM), Nagoya University 1 , Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3803-3070","authenticated-orcid":false,"given":"Tomokazu","family":"Kawashima","sequence":"additional","affiliation":[{"name":"University of Kentucky 2 Department of Plant and Soil Sciences , , 321 Plant Science Building, 1405 Veterans Dr., Lexington, KY 40546 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Berger","sequence":"additional","affiliation":[{"name":"Gregor Mendel Institute (GMI), Austrian Academy of Sciences 3 , Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna ,","place":["Austria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsu","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Kihara Institute for Biological Research, Yokohama City University 4 , 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7346-360X","authenticated-orcid":false,"given":"Tetsuya","family":"Higashiyama","sequence":"additional","affiliation":[{"name":"Institute of Transformative Bio-Molecules (ITbM), Nagoya University 1 , Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602 ,","place":["Japan"]},{"name":"Graduate School of Science, Nagoya University 5 Division of Biological Science , , Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5563-6741","authenticated-orcid":false,"given":"Daisuke","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Kihara Institute for Biological Research, Yokohama City University 4 , 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa 244-0813 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"2026041419080160700_JCS204123C1","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1242\/dev.068353","article-title":"Myoblast fusion: lessons from flies and mice","volume":"139","author":"Abmayr","year":"2012","journal-title":"Development"},{"key":"2026041419080160700_JCS204123C2","doi-asserted-by":"publisher","first-page":"10004","DOI":"10.1073\/pnas.1103584108","article-title":"Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis","volume":"108","author":"Adachi","year":"2011","journal-title":"Proc. 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