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This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers; however, the relationship of the vacuole to the genuine ER has remained elusive. Here, we have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion beam\/scanning electron microscopic tomography (FIB\/SEM). Studying B. abortus-infected HeLa cells and trophoblasts derived from B. melitensis-infected mice, we demonstrate that rBCVs are complex and interconnected compartments that are continuous with neighboring ER cisternae, thus supporting a model that rBCVs are extensions of genuine ER.<\/jats:p>","DOI":"10.1242\/jcs.210799","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T01:30:22Z","timestamp":1519349422000},"source":"Crossref","is-referenced-by-count":52,"title":["3D correlative electron microscopy reveals continuity of <i>Brucella<\/i>-containing vacuoles with the endoplasmic reticulum"],"prefix":"10.1242","volume":"131","author":[{"given":"Jaroslaw","family":"Sedzicki","sequence":"first","affiliation":[{"name":"Focal Area Infection Biology, Biozentrum, University of Basel 1 , 4056 Basel , Switzerland"},{"name":"Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel 2 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therese","family":"Tschon","sequence":"additional","affiliation":[{"name":"Focal Area Infection Biology, Biozentrum, University of Basel 1 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3512-433X","authenticated-orcid":false,"given":"Shyan Huey","family":"Low","sequence":"additional","affiliation":[{"name":"Focal Area Infection Biology, Biozentrum, University of Basel 1 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Willemart","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM, Unit\u00e9 de Recherche en Biologie des Microorganismes), University of Namur 3 , 5000 Namur , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth N.","family":"Goldie","sequence":"additional","affiliation":[{"name":"Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel 2 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM, Unit\u00e9 de Recherche en Biologie des Microorganismes), University of Namur 3 , 5000 Namur , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Stahlberg","sequence":"additional","affiliation":[{"name":"Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel 2 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7288-1052","authenticated-orcid":false,"given":"Christoph","family":"Dehio","sequence":"additional","affiliation":[{"name":"Focal Area Infection Biology, Biozentrum, University of Basel 1 , 4056 Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"2024061317273763900_JCS210799C1","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1242\/jcs.187419","article-title":"The cytostome-cytopharynx complex of Trypanosoma cruzi epimastigotes disassembles during cell division","volume":"130","author":"Alcantara","year":"2017","journal-title":"J. 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