{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:10:33Z","timestamp":1791483033615,"version":"4.3.4"},"reference-count":40,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T00:00:00Z","timestamp":1518652800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100008690","name":"State Fund for Fundamental Research of Ukraine","doi-asserted-by":"publisher","award":["F46.2\/001"],"award-info":[{"award-number":["F46.2\/001"]}],"id":[{"id":"10.13039\/501100008690","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004742","name":"National Academy of Sciences of Ukraine","doi-asserted-by":"publisher","award":["CPV-15\/16\/17\/18"],"award-info":[{"award-number":["CPV-15\/16\/17\/18"]}],"id":[{"id":"10.13039\/501100004742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The hippocampus is the region of the brain that is most susceptible to ischemic lesion because it contains pyramidal neurons that are highly vulnerable to ischemic cell death. A restricted brain neurogenesis limits the possibility of reversing massive cell death after stroke and, hence, endorses cell-based therapies for neuronal replacement strategies following cerebral ischemia. Neurons differentiated from neural stem\/progenitor cells (NSPCs) can mature and integrate into host circuitry, improving recovery after stroke. However, how the host environment regulates the NSPC behavior in post-ischemic tissue remains unknown. Here, we studied functional maturation of NSPCs in control and post-ischemic hippocampal tissue after modelling cerebral ischemia in situ. We traced the maturation of electrophysiological properties and integration of the NSPC-derived neurons into the host circuits, with these cells developing appropriate activity 3\u2005weeks or less after engraftment. In the tissue subjected to ischemia, the NSPC-derived neurons exhibited functional deficits, and differentiation of embryonic NSPCs to glial types \u2013 oligodendrocytes and astrocytes \u2013 was boosted. Our findings of the delayed neuronal maturation in post-ischemic conditions, while the NSPC differentiation was promoted towards glial cell types, provide new insights that could be applicable to stem cell therapy replacement strategies used after cerebral ischemia.<\/jats:p>","DOI":"10.1242\/jcs.210989","type":"journal-article","created":{"date-parts":[[2018,1,6]],"date-time":"2018-01-06T01:35:21Z","timestamp":1515202521000},"source":"Crossref","is-referenced-by-count":17,"title":["Maturation of neural stem cells and integration into hippocampal circuits \u2013 a functional study in an <i>in situ<\/i> model of cerebral ischemia"],"prefix":"10.1242","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3921-3674","authenticated-orcid":false,"given":"Olga","family":"Kopach","sequence":"first","affiliation":[{"name":"Bogomoletz Institute of Physiology 1 Department of Sensory Signalling , , Kyiv 01024 , Ukraine"},{"name":", University College London 2 Institute of Neurology , London WC1N 3BG , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oksana","family":"Rybachuk","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology 1 Department of Sensory Signalling , , Kyiv 01024 , Ukraine"},{"name":"State Institute of Genetic and Regenerative Medicine 3 , Kyiv 04114 , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volodymyr","family":"Krotov","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology 1 Department of Sensory Signalling , , Kyiv 01024 , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7636-1302","authenticated-orcid":false,"given":"Vitalii","family":"Kyryk","sequence":"additional","affiliation":[{"name":"State Institute of Genetic and Regenerative Medicine 3 , Kyiv 04114 , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2450-3134","authenticated-orcid":false,"given":"Nana","family":"Voitenko","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology 1 Department of Sensory Signalling , , Kyiv 01024 , Ukraine"},{"name":"Kyiv Academic University 4 , Kyiv 03142 , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Pivneva","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology 1 Department of Sensory Signalling , , Kyiv 01024 , Ukraine"},{"name":"State Institute of Genetic and Regenerative Medicine 3 , Kyiv 04114 , Ukraine"},{"name":"Kyiv Academic University 4 , Kyiv 03142 , Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,2,20]]},"reference":[{"key":"2024061317274134200_JCS210989C1","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.1002\/stem.1823","article-title":"Optogenetics reveal delayed afferent synaptogenesis on grafted human-induced pluripotent stem cell-derived neural progenitors","volume":"32","author":"Avaliani","year":"2014","journal-title":"Stem Cells"},{"key":"2024061317274134200_JCS210989C2","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1038\/nature03434","article-title":"Loss of cyclophilin D reveals a critical role for mitochondrial permeability transition in cell death","volume":"434","author":"Baines","year":"2005","journal-title":"Nature"},{"key":"2024061317274134200_JCS210989C3","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.stem.2015.09.002","article-title":"Rapid conversion of fibroblasts into functional forebrain GABAergic interneurons by direct genetic reprogramming","volume":"17","author":"Colasante","year":"2015","journal-title":"Cell Stem Cell"},{"key":"2024061317274134200_JCS210989C4","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/jcbfm.2010.81","article-title":"Cell number and timing of transplantation determine survival of human neural stem cell grafts in stroke-damaged rat brain","volume":"31","author":"Darsalia","year":"2011","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"2024061317274134200_JCS210989C5","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/jbiol35","article-title":"Astrocytes derived from glial-restricted precursors promote spinal cord repair","volume":"5","author":"Davies","year":"2006","journal-title":"J. Biol."},{"key":"2024061317274134200_JCS210989C6","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1186\/s13287-017-0513-6","article-title":"Stroke alters behavior of human skin-derived neural progenitors after transplantation adjacent to neurogenic area in rat brain","volume":"8","author":"de La Rosa-Prieto","year":"2017","journal-title":"Stem Cell Res. Ther."},{"key":"2024061317274134200_JCS210989C7","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1111\/j.1469-7793.2001.0521c.xd","article-title":"Neuronal mechanisms of the anoxia-induced network oscillations in the rat hippocampus in vitro","volume":"536","author":"Dzhala","year":"2001","journal-title":"J. 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