{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:20:26Z","timestamp":1770884426489,"version":"3.50.1"},"reference-count":48,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2019,8,15]],"date-time":"2019-08-15T00:00:00Z","timestamp":1565827200000},"content-version":"vor","delay-in-days":370,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["ZO1 HD001409"],"award-info":[{"award-number":["ZO1 HD001409"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Although influenza kills about a half million people each year, even after excluding pandemics, there is only one set of antiviral drugs: neuraminidase inhibitors. By using a new approach utilizing giant unilamellar vesicles and infectious X-31 influenza virus, and testing for the newly identified pore intermediate of membrane fusion, we observed \u223c30\u201387% poration, depending upon lipid composition. Testing the hypothesis that spontaneous curvature (SC) of the lipid monolayer controls membrane poration, our Poisson model and Boltzmann energetic considerations suggest a transition from a leaky to a non-leaky fusion pathway depending on the SC of the target membrane. When the target membrane SC is below approximately \u22120.20\u2005nm\u22121 fusion between influenza virus and target membrane is predominantly non-leaky while above that fusion is predominantly leaky, suggesting that influenza hemagglutinin (HA)-catalyzed topological conversion of target membranes during fusion is associated with a loss of membrane integrity.<\/jats:p>","DOI":"10.1242\/jcs.218321","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T10:09:21Z","timestamp":1530526161000},"source":"Crossref","is-referenced-by-count":47,"title":["Lipid-dependence of target membrane stability during influenza viral fusion"],"prefix":"10.1242","volume":"132","author":[{"given":"Sourav","family":"Haldar","sequence":"first","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development Section on Integrative Biophysics , , Bethesda, MD 20892 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Mekhedov","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development Section on Integrative Biophysics , , Bethesda, MD 20892 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad D.","family":"McCormick","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development Section on Integrative Biophysics , , Bethesda, MD 20892 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul S.","family":"Blank","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development Section on Integrative Biophysics , , Bethesda, MD 20892 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1707-3789","authenticated-orcid":false,"given":"Joshua","family":"Zimmerberg","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development Section on Integrative Biophysics , , Bethesda, MD 20892 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2018,8,10]]},"reference":[{"key":"2024061318274048900_JCS218321C1","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/0005-2736(94)90095-7","article-title":"On the validity of lipid dequenching assays for estimating virus fusion kinetics","volume":"1190","author":"Arbuzova","year":"1994","journal-title":"Biochim. 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