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Defects in IFT cause several human diseases. IFT trains contain the complexes IFT-A and IFT-B. To dissect the functions of these complexes, we studied a Chlamydomonas mutant that is null for the IFT-A protein IFT140. The mutation had no effect on IFT-B but destabilized IFT-A, preventing flagella assembly. Therefore, IFT-A assembly requires IFT140. Truncated IFT140, which lacks the N-terminal WD repeats of the protein, partially rescued IFT and supported formation of half-length flagella that contained normal levels of IFT-B but greatly reduced amounts of IFT-A. The axonemes of these flagella had normal ultrastructure and, as investigated by SDS-PAGE, normal composition. However, composition of the flagellar \u2018membrane+matrix\u2019 was abnormal. Analysis of the latter fraction by mass spectrometry revealed decreases in small GTPases, lipid-anchored proteins and cell signaling proteins. Thus, IFT-A is specialized for the import of membrane-associated proteins. Abnormal levels of the latter are likely to account for the multiple phenotypes of patients with defects in IFT140.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.220749","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T17:55:14Z","timestamp":1547834114000},"source":"Crossref","is-referenced-by-count":85,"title":["A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia"],"prefix":"10.1242","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-1148","authenticated-orcid":false,"given":"Tyler","family":"Picariello","sequence":"first","affiliation":[{"name":"University of Massachusetts Medical School 1 Division of Cell Biology and Imaging, Department of Radiology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Salem State University 2 Department of Biology , , Salem, MA 01970 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Hou","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 1 Division of Cell Biology and Imaging, Department of Radiology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8256-5336","authenticated-orcid":false,"given":"Gregory","family":"Swank","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 1 Division of Cell Biology and Imaging, Department of Radiology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah A.","family":"Cochran","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 1 Division of Cell Biology and Imaging, Department of Radiology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver D.","family":"King","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 3 Department of Neurology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-6470","authenticated-orcid":false,"given":"Karl","family":"Lechtreck","sequence":"additional","affiliation":[{"name":"University of Georgia 4 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Pazour","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 5 Program in Molecular Medicine , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9497-9218","authenticated-orcid":false,"given":"George B.","family":"Witman","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School 1 Division of Cell Biology and Imaging, Department of Radiology , , Worcester, MA 01655 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"2024061318201596300_JCS220749C1","doi-asserted-by":"publisher","first-page":"3704","DOI":"10.1242\/jcs.035626","article-title":"Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei","volume":"121","author":"Absalon","year":"2008","journal-title":"J. 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