{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T22:51:30Z","timestamp":1781563890788,"version":"3.54.5"},"reference-count":30,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD088412"],"award-info":[{"award-number":["R01HD088412"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["JP15H05573"],"award-info":[{"award-number":["JP15H05573"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1160866"],"award-info":[{"award-number":["OPP1160866"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Chemokines are signaling proteins that are secreted to induce chemotaxis during immunological events. However, the functions of transmembrane-type chemokine-like factor (CKLF) and the protein family CMTM (CKLF-like MARVEL transmembrane domain containing) remain to be determined. In this study, we focused on the testis-specific mouse Cmtm gene cluster (Cmtm1, Cmtm2a, and Cmtm2b) and generated CRISPR\/Cas9-mediated mutant mice to examine its physiological functions. Although Cmtm1 mutant mice were fertile, Cmtm2a and Cmtm2b double mutant mice had defects in male fertility due to impaired sperm function. We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.<\/jats:p>","DOI":"10.1242\/jcs.221481","type":"journal-article","created":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T09:49:04Z","timestamp":1536745744000},"source":"Crossref","is-referenced-by-count":34,"title":["Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8332-3507","authenticated-orcid":false,"given":"Yoshitaka","family":"Fujihara","sequence":"first","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asami","family":"Oji","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Kojima-Kita","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Larasati","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":false,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"2021042422362575000_JCS221481C1","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/B978-0-12-801185-0.00015-5","article-title":"CRISPR\/Cas9-based genome editing in mice by single plasmid injection","volume":"546","author":"Fujihara","year":"2014","journal-title":"Methods Enzymol."},{"key":"2021042422362575000_JCS221481C2","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1242\/jcs.067363","article-title":"Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse","volume":"123","author":"Fujihara","year":"2010","journal-title":"J. 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